A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury
Abstract Current diagnostics for sepsis‐associated acute kidney injury (SA‐AKI) detect kidney damage only at advanced stages, limiting opportunities for timely intervention. A DNA origami‐based nanoplatform is developed for the early diagnosis and treatment of SA‐AKI. Modified with a fluorophore (Cy...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202416330 |
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| author | Yingying Zhao Yadan Zhao Yufan Ling Zhiming Chen Xiaofeng Wu Xing Lu Yao He Houyu Wang Fenglin Dong |
| author_facet | Yingying Zhao Yadan Zhao Yufan Ling Zhiming Chen Xiaofeng Wu Xing Lu Yao He Houyu Wang Fenglin Dong |
| author_sort | Yingying Zhao |
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| description | Abstract Current diagnostics for sepsis‐associated acute kidney injury (SA‐AKI) detect kidney damage only at advanced stages, limiting opportunities for timely intervention. A DNA origami‐based nanoplatform is developed for the early diagnosis and treatment of SA‐AKI. Modified with a fluorophore (Cy5) and quencher (BHQ3), the DNA origami remains nonfluorescent under normal conditions. During SA‐AKI, elevated microRNA‐21 triggers a strand displacement reaction that restores the fluorescence signal, enabling real‐time detection. Additionally, the photoacoustic changes of BHQ3, driven by different excretion rates of the nanostructure and released DNA strands, enable dual‐mode imaging, enhancing diagnostic accuracy. Therapeutically, DNA origami scavenges reactive oxygen species and, when conjugated with the antimicrobial peptide Leucine‐Leucine‐37 (LL‐37), exhibits bactericidal effects. This combination boosts survival rates by 80% in SA‐AKI models. This dual‐response nanoplatform integrates precise imaging and targeted therapy, offering a powerful strategy for SA‐AKI management and advancing applications of DNA origami in precision nanomedicine. |
| format | Article |
| id | doaj-art-2d3685fb94f24c8baf783d90cd5c7d4e |
| institution | OA Journals |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-2d3685fb94f24c8baf783d90cd5c7d4e2025-08-20T02:18:32ZengWileyAdvanced Science2198-38442025-04-011216n/an/a10.1002/advs.202416330A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney InjuryYingying Zhao0Yadan Zhao1Yufan Ling2Zhiming Chen3Xiaofeng Wu4Xing Lu5Yao He6Houyu Wang7Fenglin Dong8Department of Ultrasound the First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 ChinaSuzhou Key Laboratory of Nanotechnology and Biomedicine Institute of Functional Nano and Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon‐Based Functional Materials and Devices Soochow University Suzhou Jiangsu 215123 ChinaState Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and Protection Soochow University Suzhou 215123 ChinaDepartment of Ultrasound Children‘s Hospital of Soochow University Suzhou Jiangsu 215000 ChinaDepartment of Ultrasound the First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 ChinaSuzhou Key Laboratory of Nanotechnology and Biomedicine Institute of Functional Nano and Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon‐Based Functional Materials and Devices Soochow University Suzhou Jiangsu 215123 ChinaSuzhou Key Laboratory of Nanotechnology and Biomedicine Institute of Functional Nano and Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon‐Based Functional Materials and Devices Soochow University Suzhou Jiangsu 215123 ChinaSuzhou Key Laboratory of Nanotechnology and Biomedicine Institute of Functional Nano and Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon‐Based Functional Materials and Devices Soochow University Suzhou Jiangsu 215123 ChinaDepartment of Ultrasound the First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 ChinaAbstract Current diagnostics for sepsis‐associated acute kidney injury (SA‐AKI) detect kidney damage only at advanced stages, limiting opportunities for timely intervention. A DNA origami‐based nanoplatform is developed for the early diagnosis and treatment of SA‐AKI. Modified with a fluorophore (Cy5) and quencher (BHQ3), the DNA origami remains nonfluorescent under normal conditions. During SA‐AKI, elevated microRNA‐21 triggers a strand displacement reaction that restores the fluorescence signal, enabling real‐time detection. Additionally, the photoacoustic changes of BHQ3, driven by different excretion rates of the nanostructure and released DNA strands, enable dual‐mode imaging, enhancing diagnostic accuracy. Therapeutically, DNA origami scavenges reactive oxygen species and, when conjugated with the antimicrobial peptide Leucine‐Leucine‐37 (LL‐37), exhibits bactericidal effects. This combination boosts survival rates by 80% in SA‐AKI models. This dual‐response nanoplatform integrates precise imaging and targeted therapy, offering a powerful strategy for SA‐AKI management and advancing applications of DNA origami in precision nanomedicine.https://doi.org/10.1002/advs.202416330DNA origamidual‐modal imagingreactive oxygen speciessepsis‐associated acute kidney injury |
| spellingShingle | Yingying Zhao Yadan Zhao Yufan Ling Zhiming Chen Xiaofeng Wu Xing Lu Yao He Houyu Wang Fenglin Dong A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury Advanced Science DNA origami dual‐modal imaging reactive oxygen species sepsis‐associated acute kidney injury |
| title | A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury |
| title_full | A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury |
| title_fullStr | A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury |
| title_full_unstemmed | A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury |
| title_short | A Dual‐Response DNA Origami Platform for Imaging and Treatment of Sepsis‐Associated Acute Kidney Injury |
| title_sort | dual response dna origami platform for imaging and treatment of sepsis associated acute kidney injury |
| topic | DNA origami dual‐modal imaging reactive oxygen species sepsis‐associated acute kidney injury |
| url | https://doi.org/10.1002/advs.202416330 |
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