Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent
<p><em>Rhododendron</em> has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from <em>Rodo...
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Universitas Negeri Semarang
2017-12-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/10323 |
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| author | Risa Fitriandini Sri Budiarti Yulin Lestari |
| author_facet | Risa Fitriandini Sri Budiarti Yulin Lestari |
| author_sort | Risa Fitriandini |
| collection | DOAJ |
| description | <p><em>Rhododendron</em> has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from <em>Rododendron </em>spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from <em>Rhododendron </em>spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic <em>Escerichia coli </em>(EPEC) K1.1 resistant to ampicillin and <em>Bacillus pumilus</em>. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 <em>Rhododendron</em> species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against <em>B</em>.<em> pumilus</em>, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 <sup>o</sup>C. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against <em>B. pumilus</em><em> </em>was at 1250 µg/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated <em>B. pumilus</em> showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from <em>Rhododendron </em>spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent. </p> |
| format | Article |
| id | doaj-art-2d26bec1d1b24e208aa5525adbe1bc8b |
| institution | Kabale University |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-2d26bec1d1b24e208aa5525adbe1bc8b2025-08-20T03:55:17ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102017-12-019360060710.15294/biosaintifika.v9i3.103236834Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial AgentRisa Fitriandini0Sri Budiarti1Yulin Lestari2Graduate School, Institut Pertanian BogorDepartement of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian BogorDepartement of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor<p><em>Rhododendron</em> has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from <em>Rododendron </em>spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from <em>Rhododendron </em>spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic <em>Escerichia coli </em>(EPEC) K1.1 resistant to ampicillin and <em>Bacillus pumilus</em>. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 <em>Rhododendron</em> species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against <em>B</em>.<em> pumilus</em>, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 <sup>o</sup>C. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against <em>B. pumilus</em><em> </em>was at 1250 µg/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated <em>B. pumilus</em> showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from <em>Rhododendron </em>spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent. </p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/10323ActinobacteriaAntibacterial activityEndophyteRhododendron spp. |
| spellingShingle | Risa Fitriandini Sri Budiarti Yulin Lestari Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent Biosaintifika: Journal of Biology & Biology Education Actinobacteria Antibacterial activity Endophyte Rhododendron spp. |
| title | Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent |
| title_full | Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent |
| title_fullStr | Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent |
| title_full_unstemmed | Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent |
| title_short | Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent |
| title_sort | endophytic actinobacteria from rhododendron spp as an antibacterial agent |
| topic | Actinobacteria Antibacterial activity Endophyte Rhododendron spp. |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/10323 |
| work_keys_str_mv | AT risafitriandini endophyticactinobacteriafromrhododendronsppasanantibacterialagent AT sribudiarti endophyticactinobacteriafromrhododendronsppasanantibacterialagent AT yulinlestari endophyticactinobacteriafromrhododendronsppasanantibacterialagent |