Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles
Programmed cell death (PCD) plays a crucial role in the biological processes of living organisms and occurs in various forms, such as apoptosis, necroptosis and ferroptosis. However, traditional methods for PCD analysis are time-consuming and complex. In this paper, we propose a facile surface-enhan...
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| Language: | English |
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World Scientific Publishing
2025-07-01
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| Series: | Journal of Innovative Optical Health Sciences |
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| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545824420033 |
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| author | Jieyi Chen Binggang Ye Ranran Fan Jiaqi Liu Yaru Han Haolin Chen Wen Zhang Zhouyi Guo Zhiming Liu Huiqing Zhong |
| author_facet | Jieyi Chen Binggang Ye Ranran Fan Jiaqi Liu Yaru Han Haolin Chen Wen Zhang Zhouyi Guo Zhiming Liu Huiqing Zhong |
| author_sort | Jieyi Chen |
| collection | DOAJ |
| description | Programmed cell death (PCD) plays a crucial role in the biological processes of living organisms and occurs in various forms, such as apoptosis, necroptosis and ferroptosis. However, traditional methods for PCD analysis are time-consuming and complex. In this paper, we propose a facile surface-enhanced Raman spectroscopy (SERS)-based strategy for the real-time analysis of three PCD patterns utilizing black phosphorus–gold nanoparticles (BP–Au NPs) as the ultrasensitive unlabeled Raman probe. BP–Au NPs, which possess excellent biocompatibility, are capable of detecting dye molecules at concentrations as low as [Formula: see text][Formula: see text]M and remain stable for at least one week in different physiological environments. In view of this, BP–Au NPs-based SERS technique can distinguish the tiny differences in the molecular fingerprints of cancer cells undergoing three PCD patterns (apoptosis, necroptosis and ferroptosis) triggered by doxorubicin, shikonin and erastin, respectively. We also have real-time monitoring of the intracellular molecular events during PCD, which spy the fluctuations of some typical SERS bands assigned to protein, DNA and lipid, revealing the unique phenotypic characteristics of each PCD pattern. This strategy provides a detailed and comprehensive analysis of the mechanisms of drug-induced PCD at the Raman level. |
| format | Article |
| id | doaj-art-2d233bf99d034d48b0f8257a83b1be29 |
| institution | DOAJ |
| issn | 1793-5458 1793-7205 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj-art-2d233bf99d034d48b0f8257a83b1be292025-08-20T03:09:03ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052025-07-01180410.1142/S1793545824420033Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticlesJieyi Chen0Binggang Ye1Ranran Fan2Jiaqi Liu3Yaru Han4Haolin Chen5Wen Zhang6Zhouyi Guo7Zhiming Liu8Huiqing Zhong9MOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaHealthy Medical Engineering Technology Research Center, Guangdong Food and Drug Vocational College, Guangzhou 510520, P. R. ChinaMOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaDepartment of Anesthesiology, General Hospital of Southern Theater Command of People’s Liberation Army, Guangzhou 510010, P. R. ChinaResearch Center of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P. R. ChinaMOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science and Guangdong, Provincial Key Laboratory of Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaProgrammed cell death (PCD) plays a crucial role in the biological processes of living organisms and occurs in various forms, such as apoptosis, necroptosis and ferroptosis. However, traditional methods for PCD analysis are time-consuming and complex. In this paper, we propose a facile surface-enhanced Raman spectroscopy (SERS)-based strategy for the real-time analysis of three PCD patterns utilizing black phosphorus–gold nanoparticles (BP–Au NPs) as the ultrasensitive unlabeled Raman probe. BP–Au NPs, which possess excellent biocompatibility, are capable of detecting dye molecules at concentrations as low as [Formula: see text][Formula: see text]M and remain stable for at least one week in different physiological environments. In view of this, BP–Au NPs-based SERS technique can distinguish the tiny differences in the molecular fingerprints of cancer cells undergoing three PCD patterns (apoptosis, necroptosis and ferroptosis) triggered by doxorubicin, shikonin and erastin, respectively. We also have real-time monitoring of the intracellular molecular events during PCD, which spy the fluctuations of some typical SERS bands assigned to protein, DNA and lipid, revealing the unique phenotypic characteristics of each PCD pattern. This strategy provides a detailed and comprehensive analysis of the mechanisms of drug-induced PCD at the Raman level.https://www.worldscientific.com/doi/10.1142/S1793545824420033Programmed cell deathsurface-enhanced Raman spectroscopyblack phosphorus–gold nanoparticles |
| spellingShingle | Jieyi Chen Binggang Ye Ranran Fan Jiaqi Liu Yaru Han Haolin Chen Wen Zhang Zhouyi Guo Zhiming Liu Huiqing Zhong Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles Journal of Innovative Optical Health Sciences Programmed cell death surface-enhanced Raman spectroscopy black phosphorus–gold nanoparticles |
| title | Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles |
| title_full | Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles |
| title_fullStr | Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles |
| title_full_unstemmed | Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles |
| title_short | Real-time SERS analysis of programmed cell death patterns based on black Phosphorus–gold nanoparticles |
| title_sort | real time sers analysis of programmed cell death patterns based on black phosphorus gold nanoparticles |
| topic | Programmed cell death surface-enhanced Raman spectroscopy black phosphorus–gold nanoparticles |
| url | https://www.worldscientific.com/doi/10.1142/S1793545824420033 |
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