Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China

Objective: To uncover the characteristics of tick-borne viruses in Guizhou Province. Methods: A total of 414 Rhipicephalus microplus were collected from 5 counties in Guizhou Province, China from August 2022 to October 2023. A group of 12 ticks from each study sites was sequenced by next generation...

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Main Authors: Jixia Tang, Qiu Chen, Jiao Meng, Shenchun Wu, Chaomin Zhou, Yisong Dai, Xingxing Chen, Jiafu Jiang, Sun Yi, Wuchun Cao, Fuxun Yu, Jiahong Wu, Lin Zhan
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-05-01
Series:Asian Pacific Journal of Tropical Medicine
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Online Access:https://journals.lww.com/10.4103/apjtm.apjtm_667_24
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author Jixia Tang
Qiu Chen
Jiao Meng
Shenchun Wu
Chaomin Zhou
Yisong Dai
Xingxing Chen
Jiafu Jiang
Sun Yi
Wuchun Cao
Fuxun Yu
Jiahong Wu
Lin Zhan
author_facet Jixia Tang
Qiu Chen
Jiao Meng
Shenchun Wu
Chaomin Zhou
Yisong Dai
Xingxing Chen
Jiafu Jiang
Sun Yi
Wuchun Cao
Fuxun Yu
Jiahong Wu
Lin Zhan
author_sort Jixia Tang
collection DOAJ
description Objective: To uncover the characteristics of tick-borne viruses in Guizhou Province. Methods: A total of 414 Rhipicephalus microplus were collected from 5 counties in Guizhou Province, China from August 2022 to October 2023. A group of 12 ticks from each study sites was sequenced by next generation sequencing. Results: 8 contigs of Wuhan mivirus (Chuviridae, Mivirus) with the length of 2094 bp to 11 580 bp and 4 contigs of Lihan tick virus (Phenuiviridae, Uukuvirus) with the length of 1 401 bp to 7 080 bp were obtained, respectively. The prevalence rate of Wuhan mivirus and Lihan tick virus was 51.98% and 11.30%, respectively. The identities of gene sequences of both Wuhan mivirus and Lihan tick virus were 94%-100% compared with sequences in the National Center for Biotechnology Information. The phylogenetic analysis indicated that the Wuhan mivirus detected in this study was in the same branch with the Wuhan mivirus of Sichuan isolate TIGMIC-27 (NCBI Accession: OP628598) and Zhejiang isolate TIGMIC-45 (NCBI Accession: OP628613). In addition, the Lihan tick virus was in the same branch with the Sichuan Lihan tick virus isolate TIGMIC-46 (NCBI Accession: ON812358). Conclusions: Both Wuhan mivirus and Lihan tick virus were prevalent in Rhipicephalus microplus in Guizhou Province. More studies are needed to understand the pathogenicity and public health threats of these tick-borne viruses.
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publisher Wolters Kluwer Medknow Publications
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spelling doaj-art-2d22fefad74a493fa3ce66b3c99643132025-08-20T02:32:15ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462025-05-0118521021710.4103/apjtm.apjtm_667_24Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, ChinaJixia TangQiu ChenJiao MengShenchun WuChaomin ZhouYisong DaiXingxing ChenJiafu JiangSun YiWuchun CaoFuxun YuJiahong WuLin ZhanObjective: To uncover the characteristics of tick-borne viruses in Guizhou Province. Methods: A total of 414 Rhipicephalus microplus were collected from 5 counties in Guizhou Province, China from August 2022 to October 2023. A group of 12 ticks from each study sites was sequenced by next generation sequencing. Results: 8 contigs of Wuhan mivirus (Chuviridae, Mivirus) with the length of 2094 bp to 11 580 bp and 4 contigs of Lihan tick virus (Phenuiviridae, Uukuvirus) with the length of 1 401 bp to 7 080 bp were obtained, respectively. The prevalence rate of Wuhan mivirus and Lihan tick virus was 51.98% and 11.30%, respectively. The identities of gene sequences of both Wuhan mivirus and Lihan tick virus were 94%-100% compared with sequences in the National Center for Biotechnology Information. The phylogenetic analysis indicated that the Wuhan mivirus detected in this study was in the same branch with the Wuhan mivirus of Sichuan isolate TIGMIC-27 (NCBI Accession: OP628598) and Zhejiang isolate TIGMIC-45 (NCBI Accession: OP628613). In addition, the Lihan tick virus was in the same branch with the Sichuan Lihan tick virus isolate TIGMIC-46 (NCBI Accession: ON812358). Conclusions: Both Wuhan mivirus and Lihan tick virus were prevalent in Rhipicephalus microplus in Guizhou Province. More studies are needed to understand the pathogenicity and public health threats of these tick-borne viruses.https://journals.lww.com/10.4103/apjtm.apjtm_667_24tick-borne viruswuhan miviruslihan tick virusrhipicephalus microplusmetagenomicsguizhou province
spellingShingle Jixia Tang
Qiu Chen
Jiao Meng
Shenchun Wu
Chaomin Zhou
Yisong Dai
Xingxing Chen
Jiafu Jiang
Sun Yi
Wuchun Cao
Fuxun Yu
Jiahong Wu
Lin Zhan
Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China
Asian Pacific Journal of Tropical Medicine
tick-borne virus
wuhan mivirus
lihan tick virus
rhipicephalus microplus
metagenomics
guizhou province
title Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China
title_full Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China
title_fullStr Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China
title_full_unstemmed Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China
title_short Tick-borne Wuhan mivirus and Lihan tick virus in Rhipicephalus microplus in Guizhou Province, China
title_sort tick borne wuhan mivirus and lihan tick virus in rhipicephalus microplus in guizhou province china
topic tick-borne virus
wuhan mivirus
lihan tick virus
rhipicephalus microplus
metagenomics
guizhou province
url https://journals.lww.com/10.4103/apjtm.apjtm_667_24
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