Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap
Background/Objectives: Considering the large number of candidates in vaccine-testing studies against different pathogens and the amount of time spent in the preclinical and clinical trials, there is a pressing need to develop an improved in vivo system to quickly screen vaccine candidates. The model...
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MDPI AG
2024-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/12/12/1322 |
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| author | Mariana Ferreira Lanna Lucilene Aparecida Resende Paula Mello De Luca Wanessa Moreira Goes Maykelin Fuentes Zaldívar André Tetzl Costa Walderez Ornelas Dutra Alexandre Barbosa Reis Olindo Assis Martins-Filho Kenneth Jhon Gollob Sandra Aparecida Lima de Moura Edelberto Santos Dias Érika Michalsky Monteiro Denise Silveira-Lemos Rodolfo Cordeiro Giunchetti |
| author_facet | Mariana Ferreira Lanna Lucilene Aparecida Resende Paula Mello De Luca Wanessa Moreira Goes Maykelin Fuentes Zaldívar André Tetzl Costa Walderez Ornelas Dutra Alexandre Barbosa Reis Olindo Assis Martins-Filho Kenneth Jhon Gollob Sandra Aparecida Lima de Moura Edelberto Santos Dias Érika Michalsky Monteiro Denise Silveira-Lemos Rodolfo Cordeiro Giunchetti |
| author_sort | Mariana Ferreira Lanna |
| collection | DOAJ |
| description | Background/Objectives: Considering the large number of candidates in vaccine-testing studies against different pathogens and the amount of time spent in the preclinical and clinical trials, there is a pressing need to develop an improved in vivo system to quickly screen vaccine candidates. The model of a polyester–polyurethane sponge implant provides a rapid analysis of the specific stimulus–response, allowing the study of a compartmentalized microenvironment. The sponge implant’s defined measurements were standardized as a compartment to assess the immune response triggered by the vaccinal antigen. The LBSap vaccine (composed of <i>Leishmania braziliensis</i> antigens associated with saponin adjuvant) was used in the sponge model to assess the antigen-specific immunological biomarker, including memory generation after initial contact with the antigen. Methods: Mice strains (Swiss, BALB/c, and C57BL/6) were previously immunized using LBSap vaccine, followed by an antigenic booster performed inside the sponge implant. The sponge implants were assessed after 72 h, and the immune response pattern was analyzed according to leukocyte immunophenotyping and cytokine production. Results: After LBSap vaccination, the innate immune response of the antigenic booster in the sponge implants demonstrated higher levels in the Ly+ neutrophils and CD11c+ dendritic cells with reduced numbers of F4/80+ macrophages. Moreover, the adaptive immune response in Swiss mice demonstrated a high CD3+CD4+ T-cell frequency, consisting of an effector memory component, in addition to a cytoxicity response (CD3+CD8+ T cells), displaying the central memory biomarker. The major cell surface biomarker in the BALB/c mice strain was related to CD3+CD4+ effector memory, while the increased CD3+CD8+ effector memory was highlighted in C57/BL6. The cytokine profile was more inflammatory in Swiss mice, with the highest levels of IL-6, TNF, IFN-g, and IL-17, while the same cytokine was observed in in C57BL/6 yet modulated by enhanced IL-10 levels. Similar to Swiss mice, BALB/c mice triggered an inflammatory environment after the antigenic booster in the sponge implant with the increased levels in the ILL-6, TNF, and IFN-g. Conclusions: The findings emphasized the impact of genetic background on the populations engaged in immune responses, suggesting that this model can be utilized to enhance and track both innate and adaptive immune responses in vaccine candidates. Consequently, these results may inform the selection of the most suitable experimental model for biomolecule testing, taking into account how the unique characteristics of each mouse strain affect the immune response dynamics. |
| format | Article |
| id | doaj-art-2d13ddd9851c4f71bae9fb7d95fb27ec |
| institution | DOAJ |
| issn | 2076-393X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-2d13ddd9851c4f71bae9fb7d95fb27ec2025-08-20T02:57:04ZengMDPI AGVaccines2076-393X2024-11-011212132210.3390/vaccines12121322Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSapMariana Ferreira Lanna0Lucilene Aparecida Resende1Paula Mello De Luca2Wanessa Moreira Goes3Maykelin Fuentes Zaldívar4André Tetzl Costa5Walderez Ornelas Dutra6Alexandre Barbosa Reis7Olindo Assis Martins-Filho8Kenneth Jhon Gollob9Sandra Aparecida Lima de Moura10Edelberto Santos Dias11Érika Michalsky Monteiro12Denise Silveira-Lemos13Rodolfo Cordeiro Giunchetti14Laboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilInstituto Oswaldo Cruz (IOC), FIOCRUZ Av. Brasil, Rio de Janeiro 21040-900, RJ, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilImmunopathology Laboratory, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, MG, BrazilIntegrated Biomarker Research Group, René Rachou Research Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, BrazilAlbert Einstein Israeli Institute of Education and Research, Albert Einstein Hospital, São Paulo 05652-900, SP, BrazilBiomaterials and Experimental Pathology Laboratory, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, MG, BrazilTaxonomy of Phlebotomines/Epidemiology, Diagnosis and Control of Leishmaniasis Group, René Rachou Research Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, BrazilTaxonomy of Phlebotomines/Epidemiology, Diagnosis and Control of Leishmaniasis Group, René Rachou Research Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratory of Biology of Cellular Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, BrazilBackground/Objectives: Considering the large number of candidates in vaccine-testing studies against different pathogens and the amount of time spent in the preclinical and clinical trials, there is a pressing need to develop an improved in vivo system to quickly screen vaccine candidates. The model of a polyester–polyurethane sponge implant provides a rapid analysis of the specific stimulus–response, allowing the study of a compartmentalized microenvironment. The sponge implant’s defined measurements were standardized as a compartment to assess the immune response triggered by the vaccinal antigen. The LBSap vaccine (composed of <i>Leishmania braziliensis</i> antigens associated with saponin adjuvant) was used in the sponge model to assess the antigen-specific immunological biomarker, including memory generation after initial contact with the antigen. Methods: Mice strains (Swiss, BALB/c, and C57BL/6) were previously immunized using LBSap vaccine, followed by an antigenic booster performed inside the sponge implant. The sponge implants were assessed after 72 h, and the immune response pattern was analyzed according to leukocyte immunophenotyping and cytokine production. Results: After LBSap vaccination, the innate immune response of the antigenic booster in the sponge implants demonstrated higher levels in the Ly+ neutrophils and CD11c+ dendritic cells with reduced numbers of F4/80+ macrophages. Moreover, the adaptive immune response in Swiss mice demonstrated a high CD3+CD4+ T-cell frequency, consisting of an effector memory component, in addition to a cytoxicity response (CD3+CD8+ T cells), displaying the central memory biomarker. The major cell surface biomarker in the BALB/c mice strain was related to CD3+CD4+ effector memory, while the increased CD3+CD8+ effector memory was highlighted in C57/BL6. The cytokine profile was more inflammatory in Swiss mice, with the highest levels of IL-6, TNF, IFN-g, and IL-17, while the same cytokine was observed in in C57BL/6 yet modulated by enhanced IL-10 levels. Similar to Swiss mice, BALB/c mice triggered an inflammatory environment after the antigenic booster in the sponge implant with the increased levels in the ILL-6, TNF, and IFN-g. Conclusions: The findings emphasized the impact of genetic background on the populations engaged in immune responses, suggesting that this model can be utilized to enhance and track both innate and adaptive immune responses in vaccine candidates. Consequently, these results may inform the selection of the most suitable experimental model for biomolecule testing, taking into account how the unique characteristics of each mouse strain affect the immune response dynamics.https://www.mdpi.com/2076-393X/12/12/1322sponge implant modelvaccineimmune responsemice |
| spellingShingle | Mariana Ferreira Lanna Lucilene Aparecida Resende Paula Mello De Luca Wanessa Moreira Goes Maykelin Fuentes Zaldívar André Tetzl Costa Walderez Ornelas Dutra Alexandre Barbosa Reis Olindo Assis Martins-Filho Kenneth Jhon Gollob Sandra Aparecida Lima de Moura Edelberto Santos Dias Érika Michalsky Monteiro Denise Silveira-Lemos Rodolfo Cordeiro Giunchetti Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap Vaccines sponge implant model vaccine immune response mice |
| title | Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap |
| title_full | Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap |
| title_fullStr | Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap |
| title_full_unstemmed | Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap |
| title_short | Application of the Sponge Model Implants in the Study of Vaccine Memory in Mice Previously Immunized with LBSap |
| title_sort | application of the sponge model implants in the study of vaccine memory in mice previously immunized with lbsap |
| topic | sponge implant model vaccine immune response mice |
| url | https://www.mdpi.com/2076-393X/12/12/1322 |
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