The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise
(1) Background: The study involves an assessment of the frequency of selected gene variants related to folate uptake and distribution (<i>FOLR1</i> rs2071010, rs630074, <i>FOLH1</i> rs61886492, <i>GGH</i> rs11545078, rs3758149 and <i>SLC19A1</i> rs1051...
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2024-12-01
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| author | Martyna Kozłowska-Wytyk Grażyna Kurzawińska Marcin Ożarowski Aleksandra E. Mrozikiewicz Piotr Olbromski Tomasz M. Karpiński Bogusław Czerny Hubert Wolski |
| author_facet | Martyna Kozłowska-Wytyk Grażyna Kurzawińska Marcin Ożarowski Aleksandra E. Mrozikiewicz Piotr Olbromski Tomasz M. Karpiński Bogusław Czerny Hubert Wolski |
| author_sort | Martyna Kozłowska-Wytyk |
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| description | (1) Background: The study involves an assessment of the frequency of selected gene variants related to folate uptake and distribution (<i>FOLR1</i> rs2071010, rs630074, <i>FOLH1</i> rs61886492, <i>GGH</i> rs11545078, rs3758149 and <i>SLC19A1</i> rs1051266) in a group of women with fetal demise in the Polish population. (2) Methods: A total of 310 subjects were enrolled in the study. There were 110 females with idiopathic recurrent miscarriages (RM), 80 with stillbirth (IUFD) and 120 healthy controls. Designated SNVs were determined by using PCR-RFLP methods. The difference in fetal demise prevalence was assessed using a chi-square test and logistic regression analysis. (3) Results: The rs630074 variant of the <i>FOLR1</i> gene is associated with a statistically significant increase in the risk of IUFD in a recessive model (OR = 2.03, 95%CI: 1.06–3.90, <i>p</i> = 0.033). The rs61886492variant f <i>FOLH1</i> is linked to an increased risk of IUFD in co-dominant (<i>p</i> = 0.030), dominant (OR = 2.62, 95%CI: 1.07–6.38, <i>p</i> = 0.032) and log-additive models (OR = 2.64, 95%CI: 1.15–6.06 <i>p</i> = 0.030). In female carriers of the <i>A</i> allele, the risk of IUFD was 2.8 times higher compared to the control group. No relationship between the mother’s genotype and the newborn’s birth weight or placental weight was observed for the studied SNVs. (4) Conclusions: Our study finds that the rs61886492 variant of the FOLH1 gene is associated with IUFD in Polish women. However, pregnancy failures have a multifactorial pathology and other genetic or environmental factors may also contribute to their complex etiology. Further research, preferably with larger groups of women from different ethnic backgrounds, is needed to confirm the results of the current study. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-2d08d33595114e2f82c174193762ebb22025-08-20T02:01:05ZengMDPI AGApplied Sciences2076-34172024-12-0114241184710.3390/app142411847The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal DemiseMartyna Kozłowska-Wytyk0Grażyna Kurzawińska1Marcin Ożarowski2Aleksandra E. Mrozikiewicz3Piotr Olbromski4Tomasz M. Karpiński5Bogusław Czerny6Hubert Wolski7Division of Obstetrics and Gynecology, Holy Family Hospital, Jarochowskiego 18, 60-235 Poznan, PolandLaboratory of Molecular Biology, Division of Perinatology, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, PolandInstitute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, PolandDepartment of Reproduction, Poznan University of Medical Sciences, Polan 33, 60-535 Poznań, PolandClinic of Operational Gynecology, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, PolandChair and Department of Medical Microbiology, Poznań University of Medical Sciences, Rokietnicka 10, 60-806 Poznań, PolandInstitute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, PolandInstitute of Medical Sciences, Academy of Applied Sciences, Kokoszków 71, 34-400 Nowy Targ, Poland(1) Background: The study involves an assessment of the frequency of selected gene variants related to folate uptake and distribution (<i>FOLR1</i> rs2071010, rs630074, <i>FOLH1</i> rs61886492, <i>GGH</i> rs11545078, rs3758149 and <i>SLC19A1</i> rs1051266) in a group of women with fetal demise in the Polish population. (2) Methods: A total of 310 subjects were enrolled in the study. There were 110 females with idiopathic recurrent miscarriages (RM), 80 with stillbirth (IUFD) and 120 healthy controls. Designated SNVs were determined by using PCR-RFLP methods. The difference in fetal demise prevalence was assessed using a chi-square test and logistic regression analysis. (3) Results: The rs630074 variant of the <i>FOLR1</i> gene is associated with a statistically significant increase in the risk of IUFD in a recessive model (OR = 2.03, 95%CI: 1.06–3.90, <i>p</i> = 0.033). The rs61886492variant f <i>FOLH1</i> is linked to an increased risk of IUFD in co-dominant (<i>p</i> = 0.030), dominant (OR = 2.62, 95%CI: 1.07–6.38, <i>p</i> = 0.032) and log-additive models (OR = 2.64, 95%CI: 1.15–6.06 <i>p</i> = 0.030). In female carriers of the <i>A</i> allele, the risk of IUFD was 2.8 times higher compared to the control group. No relationship between the mother’s genotype and the newborn’s birth weight or placental weight was observed for the studied SNVs. (4) Conclusions: Our study finds that the rs61886492 variant of the FOLH1 gene is associated with IUFD in Polish women. However, pregnancy failures have a multifactorial pathology and other genetic or environmental factors may also contribute to their complex etiology. Further research, preferably with larger groups of women from different ethnic backgrounds, is needed to confirm the results of the current study.https://www.mdpi.com/2076-3417/14/24/11847recurrent miscarriagefolate uptake and distributiongene variants |
| spellingShingle | Martyna Kozłowska-Wytyk Grażyna Kurzawińska Marcin Ożarowski Aleksandra E. Mrozikiewicz Piotr Olbromski Tomasz M. Karpiński Bogusław Czerny Hubert Wolski The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise Applied Sciences recurrent miscarriage folate uptake and distribution gene variants |
| title | The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise |
| title_full | The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise |
| title_fullStr | The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise |
| title_full_unstemmed | The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise |
| title_short | The Importance of Gene Variants Related to Folate Uptake and Distribution in the Etiology of Recurrent Miscarriage and Intrauterine Fetal Demise |
| title_sort | importance of gene variants related to folate uptake and distribution in the etiology of recurrent miscarriage and intrauterine fetal demise |
| topic | recurrent miscarriage folate uptake and distribution gene variants |
| url | https://www.mdpi.com/2076-3417/14/24/11847 |
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