Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites
The family Rhinonyssidae includes endoparasitic, blood-feeding mites that are parasitic on birds and that remain largely unstudied despite their potential role as vectors or reservoirs for various pathogens, like other Dermanyssoidea. Traditionally, the taxonomy of the group has been based on morpho...
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2025-02-01
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| author | Susana A. Sánchez-Carrión Francisco J. Márquez Manuel de Rojas |
| author_facet | Susana A. Sánchez-Carrión Francisco J. Márquez Manuel de Rojas |
| author_sort | Susana A. Sánchez-Carrión |
| collection | DOAJ |
| description | The family Rhinonyssidae includes endoparasitic, blood-feeding mites that are parasitic on birds and that remain largely unstudied despite their potential role as vectors or reservoirs for various pathogens, like other Dermanyssoidea. Traditionally, the taxonomy of the group has been based on morphometric characteristics, which makes identification very difficult in many groups of closely related species. On the other hand, studies on the phylogenetic relationships within this group of mites have been neglected until the early years of the present century. In this study, twelve species belonging to five different species complexes were identified, and domains D1–D3 28S rRNA of each one were sequenced, for the first time, to investigate the sequence variation and its taxonomic implications for phylogenetic inference. Our data indicate that this molecular marker can effectively differentiate between species within the “motacillae”, “sairae”, “pari”, and “hirsti” complexes of the genus <i>Ptilonyssus</i> and the “melloi” and “columbae” complexes of the genus <i>Tinaminyssus</i>. Furthermore, the phylogenetic tree that can be derived from the domain D1–D3 28S rRNA sequences presented in this study is congruent with the current taxonomy of the Rhinonyssidae. This research calls for a reassessment of the taxonomic status of some group of species. |
| format | Article |
| id | doaj-art-2cfa65b4437e4879b90e7d173d166afa |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-2cfa65b4437e4879b90e7d173d166afa2025-08-20T02:03:32ZengMDPI AGPathogens2076-08172025-02-0114215610.3390/pathogens14020156Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid MitesSusana A. Sánchez-Carrión0Francisco J. Márquez1Manuel de Rojas2Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, SpainDepartment of Animal Biology, Vegetal Biology and Ecology, Faculty of Experimental Sciences, Universidad de Jaén, 23071 Jaén, SpainDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, SpainThe family Rhinonyssidae includes endoparasitic, blood-feeding mites that are parasitic on birds and that remain largely unstudied despite their potential role as vectors or reservoirs for various pathogens, like other Dermanyssoidea. Traditionally, the taxonomy of the group has been based on morphometric characteristics, which makes identification very difficult in many groups of closely related species. On the other hand, studies on the phylogenetic relationships within this group of mites have been neglected until the early years of the present century. In this study, twelve species belonging to five different species complexes were identified, and domains D1–D3 28S rRNA of each one were sequenced, for the first time, to investigate the sequence variation and its taxonomic implications for phylogenetic inference. Our data indicate that this molecular marker can effectively differentiate between species within the “motacillae”, “sairae”, “pari”, and “hirsti” complexes of the genus <i>Ptilonyssus</i> and the “melloi” and “columbae” complexes of the genus <i>Tinaminyssus</i>. Furthermore, the phylogenetic tree that can be derived from the domain D1–D3 28S rRNA sequences presented in this study is congruent with the current taxonomy of the Rhinonyssidae. This research calls for a reassessment of the taxonomic status of some group of species.https://www.mdpi.com/2076-0817/14/2/156mitesRhinonyssidaemolecular systematic28S rRNAphylogeny |
| spellingShingle | Susana A. Sánchez-Carrión Francisco J. Márquez Manuel de Rojas Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites Pathogens mites Rhinonyssidae molecular systematic 28S rRNA phylogeny |
| title | Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites |
| title_full | Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites |
| title_fullStr | Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites |
| title_full_unstemmed | Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites |
| title_short | Utility of 28S Ribosomal RNA Gene Domains for Molecular Classification and Phylogeny of Rhinonyssid Mites |
| title_sort | utility of 28s ribosomal rna gene domains for molecular classification and phylogeny of rhinonyssid mites |
| topic | mites Rhinonyssidae molecular systematic 28S rRNA phylogeny |
| url | https://www.mdpi.com/2076-0817/14/2/156 |
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