DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics.
To prevent the transmission of damaged genomic material between generations, cells require a system for accommodating DNA repair within their cell cycles. We have previously shown that Escherichia coli cells subject to a single, repairable site-specific DNA double-strand break (DSB) per DNA replicat...
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008473&type=printable |
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| author | Martin A White Elise Darmon Manuel A Lopez-Vernaza David R F Leach |
| author_facet | Martin A White Elise Darmon Manuel A Lopez-Vernaza David R F Leach |
| author_sort | Martin A White |
| collection | DOAJ |
| description | To prevent the transmission of damaged genomic material between generations, cells require a system for accommodating DNA repair within their cell cycles. We have previously shown that Escherichia coli cells subject to a single, repairable site-specific DNA double-strand break (DSB) per DNA replication cycle reach a new average cell length, with a negligible effect on population growth rate. We show here that this new cell size distribution is caused by a DSB repair-dependent delay in completion of cell division. This delay occurs despite unperturbed cell size regulated initiation of both chromosomal DNA replication and cell division. Furthermore, despite DSB repair altering the profile of DNA replication across the genome, the time required to complete chromosomal duplication is invariant. The delay in completion of cell division is accompanied by a DSB repair-dependent delay in individualization of sister nucleoids. We suggest that DSB repair events create inter-sister connections that persist until those chromosomes are separated by a closing septum. |
| format | Article |
| id | doaj-art-2cf0688045ee4775adc2114a2d9af9d5 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-2cf0688045ee4775adc2114a2d9af9d52025-08-20T02:11:01ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042020-01-01161e100847310.1371/journal.pgen.1008473DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics.Martin A WhiteElise DarmonManuel A Lopez-VernazaDavid R F LeachTo prevent the transmission of damaged genomic material between generations, cells require a system for accommodating DNA repair within their cell cycles. We have previously shown that Escherichia coli cells subject to a single, repairable site-specific DNA double-strand break (DSB) per DNA replication cycle reach a new average cell length, with a negligible effect on population growth rate. We show here that this new cell size distribution is caused by a DSB repair-dependent delay in completion of cell division. This delay occurs despite unperturbed cell size regulated initiation of both chromosomal DNA replication and cell division. Furthermore, despite DSB repair altering the profile of DNA replication across the genome, the time required to complete chromosomal duplication is invariant. The delay in completion of cell division is accompanied by a DSB repair-dependent delay in individualization of sister nucleoids. We suggest that DSB repair events create inter-sister connections that persist until those chromosomes are separated by a closing septum.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008473&type=printable |
| spellingShingle | Martin A White Elise Darmon Manuel A Lopez-Vernaza David R F Leach DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics. PLoS Genetics |
| title | DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics. |
| title_full | DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics. |
| title_fullStr | DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics. |
| title_full_unstemmed | DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics. |
| title_short | DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics. |
| title_sort | dna double strand break repair in escherichia coli perturbs cell division and chromosome dynamics |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1008473&type=printable |
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