Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots

The faba bean is a grain legume used for food and feed. It suffers from several yield limiting diseases. Botrytis fabae Sard is a fungal pathogen which causes chocolate spot disease in faba bean all over the world. Searching for solutions to the problem of yield loss due to B. fabae is critical. One...

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Main Authors: Gebeyehu Yibeltie Mengstie, Zewdu Teshome Awlachew, Atsede Muleta Degefa
Format: Article
Language:English
Published: Taylor & Francis Group 2023-03-01
Series:Biotechnology & Biotechnological Equipment
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Online Access:https://www.tandfonline.com/doi/10.1080/13102818.2023.2297885
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author Gebeyehu Yibeltie Mengstie
Zewdu Teshome Awlachew
Atsede Muleta Degefa
author_facet Gebeyehu Yibeltie Mengstie
Zewdu Teshome Awlachew
Atsede Muleta Degefa
author_sort Gebeyehu Yibeltie Mengstie
collection DOAJ
description The faba bean is a grain legume used for food and feed. It suffers from several yield limiting diseases. Botrytis fabae Sard is a fungal pathogen which causes chocolate spot disease in faba bean all over the world. Searching for solutions to the problem of yield loss due to B. fabae is critical. One sustainable solution is the utilization of plant-growth-promoting rhizobacteria as biocontrol agents. This study aimed to demonstrate the effect of rhizobacteria strains on controlling chocolate spots and promoting the growth of faba beans under pot conditions. Three rhizobacteria strains that previously demonstrated promising potential were used and tested for antagonistic activity against B.fabae in dual culture. All tested rhizobacterial strains inhibited the mycelial growth of B.fabae with different potential, and Chryseobacterium strain GY04 showed a maximum percent of mycelial growth inhibition of 72.38%. Also, all rhizobacteria strains significantly reduced the incidence and severity of chocolate spot disease, with different efficacy in both faba bean varieties under pot conditions. Chryseobacterium strain GY04 showed the highest disease severity reduction on the local variety at 75 (79.62%) and 90 (73.57%) days after sowing. Rhizobacterium Pseudomonas chlororaphis GY07 resulted in the highest disease severity reduction on the Dosha variety (80.12, 43.85%) at 75 and 90 days after sowing, respectively. All tested rhizobacteria improved the main plant growth parameters in both varieties. The rhizobacterial strains examined in this study could be used as biocontrol against Chocolate spot disease and as growth promoters under pot conditions.
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spelling doaj-art-2ce777dc7129401cbf84f75c6d8bc3bd2025-08-20T02:49:29ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-03-0137110.1080/13102818.2023.2297885Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in potsGebeyehu Yibeltie Mengstie0Zewdu Teshome Awlachew1Atsede Muleta Degefa2Department of Biology, College of Natural and Computational Sciences, Debark University, Debark, EthiopiaDepartment of Biology, College of Natural and Computational Sciences, University of Gondar, Gondar, EthiopiaDepartment of Biology, College of Natural and Computational Sciences, University of Gondar, Gondar, EthiopiaThe faba bean is a grain legume used for food and feed. It suffers from several yield limiting diseases. Botrytis fabae Sard is a fungal pathogen which causes chocolate spot disease in faba bean all over the world. Searching for solutions to the problem of yield loss due to B. fabae is critical. One sustainable solution is the utilization of plant-growth-promoting rhizobacteria as biocontrol agents. This study aimed to demonstrate the effect of rhizobacteria strains on controlling chocolate spots and promoting the growth of faba beans under pot conditions. Three rhizobacteria strains that previously demonstrated promising potential were used and tested for antagonistic activity against B.fabae in dual culture. All tested rhizobacterial strains inhibited the mycelial growth of B.fabae with different potential, and Chryseobacterium strain GY04 showed a maximum percent of mycelial growth inhibition of 72.38%. Also, all rhizobacteria strains significantly reduced the incidence and severity of chocolate spot disease, with different efficacy in both faba bean varieties under pot conditions. Chryseobacterium strain GY04 showed the highest disease severity reduction on the local variety at 75 (79.62%) and 90 (73.57%) days after sowing. Rhizobacterium Pseudomonas chlororaphis GY07 resulted in the highest disease severity reduction on the Dosha variety (80.12, 43.85%) at 75 and 90 days after sowing, respectively. All tested rhizobacteria improved the main plant growth parameters in both varieties. The rhizobacterial strains examined in this study could be used as biocontrol against Chocolate spot disease and as growth promoters under pot conditions.https://www.tandfonline.com/doi/10.1080/13102818.2023.2297885BiocontrolBotrytis fabaefaba beanincidencepot conditionsrhizobacteria
spellingShingle Gebeyehu Yibeltie Mengstie
Zewdu Teshome Awlachew
Atsede Muleta Degefa
Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
Biotechnology & Biotechnological Equipment
Biocontrol
Botrytis fabae
faba bean
incidence
pot conditions
rhizobacteria
title Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
title_full Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
title_fullStr Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
title_full_unstemmed Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
title_short Selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
title_sort selected rhizobacteria strains as potential growth promoters and biocontrol agents against chocolate spot disease in faba bean grown in pots
topic Biocontrol
Botrytis fabae
faba bean
incidence
pot conditions
rhizobacteria
url https://www.tandfonline.com/doi/10.1080/13102818.2023.2297885
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