Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility

In order to determine the effect of endometrial injury (EI) on in vitro fertilization (IVF) outcomes in women with unexplained subfertility and explore the relationship between EI and endometrial inflammatory cytokines, 66 women with unexplained subfertility undergoing IVF treatment were recruited....

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Main Authors: Yu Liang, Junyan Han, Chanwei Jia, Yanmin Ma, Yonglian Lan, Ying Li, Shuyu Wang
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2015/757184
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author Yu Liang
Junyan Han
Chanwei Jia
Yanmin Ma
Yonglian Lan
Ying Li
Shuyu Wang
author_facet Yu Liang
Junyan Han
Chanwei Jia
Yanmin Ma
Yonglian Lan
Ying Li
Shuyu Wang
author_sort Yu Liang
collection DOAJ
description In order to determine the effect of endometrial injury (EI) on in vitro fertilization (IVF) outcomes in women with unexplained subfertility and explore the relationship between EI and endometrial inflammatory cytokines, 66 women with unexplained subfertility undergoing IVF treatment were recruited. 38 patients in the EI group underwent EI in the mid-luteal phase of the cycle and 28 patients in the non-EI (NEI) group. According to the pregnancy outcome, the NEI and EI groups were divided into NEI-nonpregnant (NEI-NP), NEI-pregnant (NEI-P), EI-NP, and EI-P. All patients underwent aspiration of endometrial secretions immediately before embryo transfer. The concentrations of ten mediators were measured using Milliplex Magnetic Bead assay. The clinical pregnancy was significantly higher in the EI than in the NEI group. The concentrations of interleukin- (IL-) 6, IL-8, IL-12 (p70), IL-13, interferon- (IFN-) γ, monocyte chemotactic protein- (MCP-) 1, and vascular endothelial growth factor (VEGF) were significantly higher in the EI than the NEI group. The expression of IFN-γ and VEGF in the EI-P was significantly increased compared to the EI-NP group. These findings suggest that, in women with unexplained subfertility, endometrial injury might be a potential method to improve clinical pregnancy rates by promoting the expression of IFN-γ and VEGF.
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spelling doaj-art-2ce5ea5d5fc14e89a2da42006c8b67682025-08-20T02:24:18ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/757184757184Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained SubfertilityYu Liang0Junyan Han1Chanwei Jia2Yanmin Ma3Yonglian Lan4Ying Li5Shuyu Wang6Assisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, ChinaInstitute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100011, ChinaAssisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, ChinaAssisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, ChinaAssisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, ChinaAssisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, ChinaAssisted Reproduction Center of Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, ChinaIn order to determine the effect of endometrial injury (EI) on in vitro fertilization (IVF) outcomes in women with unexplained subfertility and explore the relationship between EI and endometrial inflammatory cytokines, 66 women with unexplained subfertility undergoing IVF treatment were recruited. 38 patients in the EI group underwent EI in the mid-luteal phase of the cycle and 28 patients in the non-EI (NEI) group. According to the pregnancy outcome, the NEI and EI groups were divided into NEI-nonpregnant (NEI-NP), NEI-pregnant (NEI-P), EI-NP, and EI-P. All patients underwent aspiration of endometrial secretions immediately before embryo transfer. The concentrations of ten mediators were measured using Milliplex Magnetic Bead assay. The clinical pregnancy was significantly higher in the EI than in the NEI group. The concentrations of interleukin- (IL-) 6, IL-8, IL-12 (p70), IL-13, interferon- (IFN-) γ, monocyte chemotactic protein- (MCP-) 1, and vascular endothelial growth factor (VEGF) were significantly higher in the EI than the NEI group. The expression of IFN-γ and VEGF in the EI-P was significantly increased compared to the EI-NP group. These findings suggest that, in women with unexplained subfertility, endometrial injury might be a potential method to improve clinical pregnancy rates by promoting the expression of IFN-γ and VEGF.http://dx.doi.org/10.1155/2015/757184
spellingShingle Yu Liang
Junyan Han
Chanwei Jia
Yanmin Ma
Yonglian Lan
Ying Li
Shuyu Wang
Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility
Mediators of Inflammation
title Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility
title_full Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility
title_fullStr Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility
title_full_unstemmed Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility
title_short Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility
title_sort effect of endometrial injury on secretion of endometrial cytokines and ivf outcomes in women with unexplained subfertility
url http://dx.doi.org/10.1155/2015/757184
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