Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model
Abstract Background Cyanoacrylate glues are widely used in interventional radiology as effective embolic agents due to their rapid polymerization and ability to achieve vessel occlusion. Nonetheless, concern remains regarding cast stability and potential recanalization over time. This study used mul...
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SpringerOpen
2025-04-01
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| Series: | CVIR Endovascular |
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| Online Access: | https://doi.org/10.1186/s42155-025-00549-8 |
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| author | Romaric Loffroy Kévin Guillen Olivier Chevallier Mohamed Fouad Emilie Couloumy Anne Dencausse Philippe Robert Sarah Catoen Anne-Virginie Salsac Serge Ludwig Aho-Glele Pierre-Olivier Comby |
| author_facet | Romaric Loffroy Kévin Guillen Olivier Chevallier Mohamed Fouad Emilie Couloumy Anne Dencausse Philippe Robert Sarah Catoen Anne-Virginie Salsac Serge Ludwig Aho-Glele Pierre-Olivier Comby |
| author_sort | Romaric Loffroy |
| collection | DOAJ |
| description | Abstract Background Cyanoacrylate glues are widely used in interventional radiology as effective embolic agents due to their rapid polymerization and ability to achieve vessel occlusion. Nonetheless, concern remains regarding cast stability and potential recanalization over time. This study used multiple modalities to evaluate the medium-term outcomes of Glubran®2 glue (methacryloxysulfolane and N butyl cyanoacrylate) embolisation in a rabbit renal-artery model. Methods The left renal arteries of six rabbits were embolized with 12.5% or 25% Glubran®2. In-vivo micro-CT scans were performed immediately after embolisation (M0) and ex-vivo scans and a histological assessment were done at one month (M1). Magnetic resonance imaging (MRI) was done at M1 to assess arterial occlusion and parenchymal changes. Quantitative and semi-quantitative parameters reflecting glue distribution, cast integrity, and tissue response were analysed. Statistical comparisons used non-parametric tests. Results All six embolisations were completed without complications. Micro-CT at M1 revealed significant cast resorption and fragmentation with both concentrations, but with no evidence of arterial recanalization. MRI and histology confirmed the persistent vascular occlusion with chronic ischemic changes in the renal parenchyma. Compensatory neovascularization from the renal capsule was observed, with no significant differences in histological inflammation between the two concentrations. Glue casts remained within the arterial lumens and were often surrounded by granulomatous inflammation. Conclusions Glubran®2 was effective for renal artery embolisation, even at a low concentration of 12.5%: despite partial cast resorption, the arteries remained occluded. Micro-CT proved to be a powerful tool for assessing changes in glue casts. Longer-term studies are warranted to further assess vascular remodelling and occlusion durability. |
| format | Article |
| id | doaj-art-2ce5bb68ab0e4ea3be7e79a63a6ec369 |
| institution | OA Journals |
| issn | 2520-8934 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | CVIR Endovascular |
| spelling | doaj-art-2ce5bb68ab0e4ea3be7e79a63a6ec3692025-08-20T02:19:58ZengSpringerOpenCVIR Endovascular2520-89342025-04-018111010.1186/s42155-025-00549-8Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit modelRomaric Loffroy0Kévin Guillen1Olivier Chevallier2Mohamed Fouad3Emilie Couloumy4Anne Dencausse5Philippe Robert6Sarah Catoen7Anne-Virginie Salsac8Serge Ludwig Aho-Glele9Pierre-Olivier Comby10Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University HospitalDepartment of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University HospitalDepartment of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University HospitalDepartment of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University HospitalR&D, Guerbet ResearchR&D, Guerbet ResearchR&D, Guerbet ResearchR&D, Guerbet ResearchBiomechanics and Bioengineering Laboratory, UMR CNRS 7338, Université de Technologie de CompiègneDepartment of Epidemiology, Statistics and Clinical Research, François-Mitterrand University HospitalICMUB Laboratory, UMR CNRS 6302, Université de BourgogneAbstract Background Cyanoacrylate glues are widely used in interventional radiology as effective embolic agents due to their rapid polymerization and ability to achieve vessel occlusion. Nonetheless, concern remains regarding cast stability and potential recanalization over time. This study used multiple modalities to evaluate the medium-term outcomes of Glubran®2 glue (methacryloxysulfolane and N butyl cyanoacrylate) embolisation in a rabbit renal-artery model. Methods The left renal arteries of six rabbits were embolized with 12.5% or 25% Glubran®2. In-vivo micro-CT scans were performed immediately after embolisation (M0) and ex-vivo scans and a histological assessment were done at one month (M1). Magnetic resonance imaging (MRI) was done at M1 to assess arterial occlusion and parenchymal changes. Quantitative and semi-quantitative parameters reflecting glue distribution, cast integrity, and tissue response were analysed. Statistical comparisons used non-parametric tests. Results All six embolisations were completed without complications. Micro-CT at M1 revealed significant cast resorption and fragmentation with both concentrations, but with no evidence of arterial recanalization. MRI and histology confirmed the persistent vascular occlusion with chronic ischemic changes in the renal parenchyma. Compensatory neovascularization from the renal capsule was observed, with no significant differences in histological inflammation between the two concentrations. Glue casts remained within the arterial lumens and were often surrounded by granulomatous inflammation. Conclusions Glubran®2 was effective for renal artery embolisation, even at a low concentration of 12.5%: despite partial cast resorption, the arteries remained occluded. Micro-CT proved to be a powerful tool for assessing changes in glue casts. Longer-term studies are warranted to further assess vascular remodelling and occlusion durability.https://doi.org/10.1186/s42155-025-00549-8Transcatheter arterial embolizationN-butyl cyanoacrylate glueAnimal modelMedium-term outcomes |
| spellingShingle | Romaric Loffroy Kévin Guillen Olivier Chevallier Mohamed Fouad Emilie Couloumy Anne Dencausse Philippe Robert Sarah Catoen Anne-Virginie Salsac Serge Ludwig Aho-Glele Pierre-Olivier Comby Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model CVIR Endovascular Transcatheter arterial embolization N-butyl cyanoacrylate glue Animal model Medium-term outcomes |
| title | Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model |
| title_full | Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model |
| title_fullStr | Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model |
| title_full_unstemmed | Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model |
| title_short | Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model |
| title_sort | micro ct and histological assessment of renal arterial embolization with glubran r 2 cyanoacrylate a medium term follow up study in a rabbit model |
| topic | Transcatheter arterial embolization N-butyl cyanoacrylate glue Animal model Medium-term outcomes |
| url | https://doi.org/10.1186/s42155-025-00549-8 |
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