Intratumor HIF-1α modulates production of a cachectic ligand to cause host wasting

Tumor-host interactions play critical roles in cancer-associated cachexia. Previous studies have identified several cachectic proteins secreted by tumors that impair metabolic homeostasis in multiple organs, leading to host wasting. The molecular mechanisms by which malignant tumors regulate the pro...

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Main Authors: Gen Xiao, Yingge Li, Yanhui Hu, Kai Tan, Mengyang Wang, Kerui Zhu, Mingkui San, Qian Cheng, Dilinigeer Tayier, Tingting Hu, Peixuan Dang, Jiaying Li, Chen Cheng, Norbert Perrimon, Zhiyong Yang, Wei Song
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Cell Insight
Online Access:http://www.sciencedirect.com/science/article/pii/S2772892725000215
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Summary:Tumor-host interactions play critical roles in cancer-associated cachexia. Previous studies have identified several cachectic proteins secreted by tumors that impair metabolic homeostasis in multiple organs, leading to host wasting. The molecular mechanisms by which malignant tumors regulate the production or secretion of these cachectic proteins, however, still remain largely unknown. In this study, we used different Drosophila cachexia models to investigate how malignant tumors regulate biosynthesis of ImpL2, a conserved cachectic protein that inhibits systemic insulin/IGF signaling and suppresses anabolism of host organs. Through bioinformatic and biochemical analysis, we found that hypoxia-inducible factor HIF-1α/Sima directly binds to the promoter region of ImpL2 gene for the first time, promoting its transcription in both tumors and non-tumor cells. Interestingly, expressing HphA to moderately suppress HIF-1α/Sima activity in adult yki3SA gut tumors or larval scrib1 RasV12 disc tumors sufficiently decreased ImpL2 expression and improved organ wasting, without affecting tumor growth. We further revealed conserved regulatory mechanisms conserved across species, as intratumor HIF-1α enhances the production of IGFBP-5, a mammalian homolog of fly ImpL2, contributing to organ wasting in both tumor-bearing mice and patients. Therefore, our study provides novel insights into the mechanisms by which tumors regulate production of cachectic ligands and the pathogenesis of cancer-induced cachexia.
ISSN:2772-8927