MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA
This study was conducted to evaluate and identify native cellulolytic microorganisms to tropical sanitary landfill sites in Lagos, Southwest Nigeria as well as to provide insights into the potential of autochthonous microorganisms in solid waste management. Soil and leachate samples were obtained si...
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Stefan cel Mare University of Suceava
2024-12-01
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| Series: | Food and Environment Safety |
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| Online Access: | https://fens.usv.ro/index.php/FENS/article/view/1300/1160 |
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| author | Olusola Abayomi Ojo-Omoniyi Olanike Maria Buraimoh Grace Toluwalase Jolaoluwa Opeyemi Hannah Akindusoye Racheal Oluwayemisi Fashogbon |
| author_facet | Olusola Abayomi Ojo-Omoniyi Olanike Maria Buraimoh Grace Toluwalase Jolaoluwa Opeyemi Hannah Akindusoye Racheal Oluwayemisi Fashogbon |
| author_sort | Olusola Abayomi Ojo-Omoniyi |
| collection | DOAJ |
| description | This study was conducted to evaluate and identify native cellulolytic microorganisms to tropical sanitary landfill sites in Lagos, Southwest Nigeria as well as to provide insights into the potential of autochthonous microorganisms in solid waste management. Soil and leachate samples were obtained simultaneously (0 – 30 cm depth) at the same time from three waste dumpsites in Lagos, using aseptic procedures. Thereafter, composite soil samples and leachates were generated independently and transported to the laboratory for analysis. Microorganisms were isolated from both soil and leachate samples using the serial dilution technique on sterile nutrient agar (NA) and potato dextrose agar (PDA). Subsequently, cellulase-producing microbial species were identified using conventional and standard microbiological techniques as well as by cultivation on Starch-casein-agar. Pure cultures of isolates were inoculated on sterile filter paper placed on Starch - casein agar plate. Isolates were selected based on their metabolic capabilities to utilize the filter paper for growth. Screening for utilization of aromatic acids was carried out in 250 mL conical flasks containing composition: minimal agar medium (pH 7.2), 1.0 g/L aromatic acids (vanillic), 1.0 mL trace elements, phosphate buffer and Bromothymol blue as pH indicator. The DNA of some selected isolates with cellulolytic activity were extracted and sequenced using 16S rRNA sequencing, ITS, and bioinformatics tools. Consequently, among the bacterial species, Bacillus sp. had the highest cellulose degradative ability and was the most prevalent (50%) in occurrence among bacterial species while Aspergillus sp. emerged as the most commonly occurring fungal isolate (35.7%). Data of selected sequenced cellulolytic isolates were deposited at NCBI GeneBank with Accession numbers: KP843680.1 (Vibrio tubiashii), MK748310.1 (Aspergillus aculeatinus), LC496490.1 (Aspergillus aculeatus), CP029751.1 (Staphylococcus aureus) and JX144699.1 (Bacillus mycoides). Environmental surveillance of these microorganisms with microbial synergistic capabilities, could transform solid waste management into a highly efficient biotechnological process that facilitates volume reduction, waste recycling and Bioenergy production. |
| format | Article |
| id | doaj-art-2cbecdc9ebea44bab9e90f5ce0c3bfdd |
| institution | DOAJ |
| issn | 2068-6609 2559-6381 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Stefan cel Mare University of Suceava |
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| series | Food and Environment Safety |
| spelling | doaj-art-2cbecdc9ebea44bab9e90f5ce0c3bfdd2025-08-20T03:11:42ZengStefan cel Mare University of SuceavaFood and Environment Safety2068-66092559-63812024-12-01234120MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIAOlusola Abayomi Ojo-OmoniyiOlanike Maria BuraimohGrace Toluwalase JolaoluwaOpeyemi Hannah AkindusoyeRacheal Oluwayemisi FashogbonThis study was conducted to evaluate and identify native cellulolytic microorganisms to tropical sanitary landfill sites in Lagos, Southwest Nigeria as well as to provide insights into the potential of autochthonous microorganisms in solid waste management. Soil and leachate samples were obtained simultaneously (0 – 30 cm depth) at the same time from three waste dumpsites in Lagos, using aseptic procedures. Thereafter, composite soil samples and leachates were generated independently and transported to the laboratory for analysis. Microorganisms were isolated from both soil and leachate samples using the serial dilution technique on sterile nutrient agar (NA) and potato dextrose agar (PDA). Subsequently, cellulase-producing microbial species were identified using conventional and standard microbiological techniques as well as by cultivation on Starch-casein-agar. Pure cultures of isolates were inoculated on sterile filter paper placed on Starch - casein agar plate. Isolates were selected based on their metabolic capabilities to utilize the filter paper for growth. Screening for utilization of aromatic acids was carried out in 250 mL conical flasks containing composition: minimal agar medium (pH 7.2), 1.0 g/L aromatic acids (vanillic), 1.0 mL trace elements, phosphate buffer and Bromothymol blue as pH indicator. The DNA of some selected isolates with cellulolytic activity were extracted and sequenced using 16S rRNA sequencing, ITS, and bioinformatics tools. Consequently, among the bacterial species, Bacillus sp. had the highest cellulose degradative ability and was the most prevalent (50%) in occurrence among bacterial species while Aspergillus sp. emerged as the most commonly occurring fungal isolate (35.7%). Data of selected sequenced cellulolytic isolates were deposited at NCBI GeneBank with Accession numbers: KP843680.1 (Vibrio tubiashii), MK748310.1 (Aspergillus aculeatinus), LC496490.1 (Aspergillus aculeatus), CP029751.1 (Staphylococcus aureus) and JX144699.1 (Bacillus mycoides). Environmental surveillance of these microorganisms with microbial synergistic capabilities, could transform solid waste management into a highly efficient biotechnological process that facilitates volume reduction, waste recycling and Bioenergy production.https://fens.usv.ro/index.php/FENS/article/view/1300/1160biodegradationcellulosegenomicslandfillslignocellulosesolid wastesustainable development |
| spellingShingle | Olusola Abayomi Ojo-Omoniyi Olanike Maria Buraimoh Grace Toluwalase Jolaoluwa Opeyemi Hannah Akindusoye Racheal Oluwayemisi Fashogbon MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA Food and Environment Safety biodegradation cellulose genomics landfills lignocellulose solid waste sustainable development |
| title | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA |
| title_full | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA |
| title_fullStr | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA |
| title_full_unstemmed | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA |
| title_short | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA |
| title_sort | molecular and biochemical characterization of autochthonous cellulolytic microorganisms from three landfill sites in lagos southwest nigeria |
| topic | biodegradation cellulose genomics landfills lignocellulose solid waste sustainable development |
| url | https://fens.usv.ro/index.php/FENS/article/view/1300/1160 |
| work_keys_str_mv | AT olusolaabayomiojoomoniyi molecularandbiochemicalcharacterizationofautochthonouscellulolyticmicroorganismsfromthreelandfillsitesinlagossouthwestnigeria AT olanikemariaburaimoh molecularandbiochemicalcharacterizationofautochthonouscellulolyticmicroorganismsfromthreelandfillsitesinlagossouthwestnigeria AT gracetoluwalasejolaoluwa molecularandbiochemicalcharacterizationofautochthonouscellulolyticmicroorganismsfromthreelandfillsitesinlagossouthwestnigeria AT opeyemihannahakindusoye molecularandbiochemicalcharacterizationofautochthonouscellulolyticmicroorganismsfromthreelandfillsitesinlagossouthwestnigeria AT rachealoluwayemisifashogbon molecularandbiochemicalcharacterizationofautochthonouscellulolyticmicroorganismsfromthreelandfillsitesinlagossouthwestnigeria |