Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain
Very high concentrations of acetic acid at low pH induce programmed cell death (PCD) in both the experimental model Saccharomyces cerevisiae and in Zygosaccharomyces bailii, the latter being considered the most problematic acidic food spoilage yeast due to its remarkable intrinsic resistance to this...
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Shared Science Publishers OG
2016-01-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/mitochondrial-proteomics-of-the-acetic-acid-induced-programmed-cell-death-response-in-a-highly-tolerant-zygosaccharomyces-bailii-derived-hybrid-strain/ |
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| author | Joana F Guerreiro Belém Sampaio-Marques Renata Soares Ana Varela Coelho Cecília Leão Paula Ludovico Isabel Sá-Correia |
| author_facet | Joana F Guerreiro Belém Sampaio-Marques Renata Soares Ana Varela Coelho Cecília Leão Paula Ludovico Isabel Sá-Correia |
| author_sort | Joana F Guerreiro |
| collection | DOAJ |
| description | Very high concentrations of acetic acid at low pH induce programmed cell death (PCD) in both the experimental model Saccharomyces cerevisiae and in Zygosaccharomyces bailii, the latter being considered the most problematic acidic food spoilage yeast due to its remarkable intrinsic resistance to this food preservative. However, while the mechanisms underlying S. cerevisiae PCD induced by acetic acid have been previously examined, the corresponding molecular players remain largely unknown in Z. bailii. Also, the reason why acetic acid concentrations known to be necrotic for S. cerevisiae induce PCD with an apoptotic phenotype in Z. bailii remains to be elucidated. In this study, a 2-DE-based expression mitochondrial proteomic analysis was explored to obtain new insights into the mechanisms involved in PCD in the Z. bailii derived hybrid strain ISA1307. This allowed the quantitative assessment of expression of protein species derived from each of the parental strains, with special emphasis on the processes taking place in the mitochondria known to play a key role in acetic acid – induced PCD. A marked decrease in the content of proteins involved in mitochondrial metabolism, in particular, in respiratory metabolism (Cor1, Rip1, Lpd1, Lat1 and Pdb1), with a concomitant increase in the abundance of proteins involved in fermentation (Pdc1, Ald4, Dld3) was registered. Other differentially expressed identified proteins also suggest the involvement of the oxidative stress response, protein translation, amino acid and nucleotide metabolism, among other processes, in the PCD response. Overall, the results strengthen the emerging concept of the importance of metabolic regulation of yeast PCD. |
| format | Article |
| id | doaj-art-2cb21cea2bb246bc85f47f58baee173a |
| institution | DOAJ |
| issn | 2311-2638 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-2cb21cea2bb246bc85f47f58baee173a2025-08-20T02:52:37ZengShared Science Publishers OGMicrobial Cell2311-26382016-01-0132657810.15698/mic2016.02.477Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strainJoana F Guerreiro0Belém Sampaio-Marques1Renata Soares2Ana Varela Coelho3Cecília Leão4Paula Ludovico5Isabel Sá-Correia6Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal.Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal.Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga 4710-057, Portugal.Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.Very high concentrations of acetic acid at low pH induce programmed cell death (PCD) in both the experimental model Saccharomyces cerevisiae and in Zygosaccharomyces bailii, the latter being considered the most problematic acidic food spoilage yeast due to its remarkable intrinsic resistance to this food preservative. However, while the mechanisms underlying S. cerevisiae PCD induced by acetic acid have been previously examined, the corresponding molecular players remain largely unknown in Z. bailii. Also, the reason why acetic acid concentrations known to be necrotic for S. cerevisiae induce PCD with an apoptotic phenotype in Z. bailii remains to be elucidated. In this study, a 2-DE-based expression mitochondrial proteomic analysis was explored to obtain new insights into the mechanisms involved in PCD in the Z. bailii derived hybrid strain ISA1307. This allowed the quantitative assessment of expression of protein species derived from each of the parental strains, with special emphasis on the processes taking place in the mitochondria known to play a key role in acetic acid – induced PCD. A marked decrease in the content of proteins involved in mitochondrial metabolism, in particular, in respiratory metabolism (Cor1, Rip1, Lpd1, Lat1 and Pdb1), with a concomitant increase in the abundance of proteins involved in fermentation (Pdc1, Ald4, Dld3) was registered. Other differentially expressed identified proteins also suggest the involvement of the oxidative stress response, protein translation, amino acid and nucleotide metabolism, among other processes, in the PCD response. Overall, the results strengthen the emerging concept of the importance of metabolic regulation of yeast PCD.http://microbialcell.com/researcharticles/mitochondrial-proteomics-of-the-acetic-acid-induced-programmed-cell-death-response-in-a-highly-tolerant-zygosaccharomyces-bailii-derived-hybrid-strain/yeast programmed cell deathZygosaccharomyces bailiiacetic acid responsequantitative proteomicsmitochondrial proteomics |
| spellingShingle | Joana F Guerreiro Belém Sampaio-Marques Renata Soares Ana Varela Coelho Cecília Leão Paula Ludovico Isabel Sá-Correia Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain Microbial Cell yeast programmed cell death Zygosaccharomyces bailii acetic acid response quantitative proteomics mitochondrial proteomics |
| title | Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain |
| title_full | Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain |
| title_fullStr | Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain |
| title_full_unstemmed | Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain |
| title_short | Mitochondrial proteomics of the acetic acid – induced programmed cell death response in a highly tolerant Zygosaccharomyces bailii – derived hybrid strain |
| title_sort | mitochondrial proteomics of the acetic acid induced programmed cell death response in a highly tolerant zygosaccharomyces bailii derived hybrid strain |
| topic | yeast programmed cell death Zygosaccharomyces bailii acetic acid response quantitative proteomics mitochondrial proteomics |
| url | http://microbialcell.com/researcharticles/mitochondrial-proteomics-of-the-acetic-acid-induced-programmed-cell-death-response-in-a-highly-tolerant-zygosaccharomyces-bailii-derived-hybrid-strain/ |
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