Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends
Insects sense intraspecific or interspecific information about the chemical substances in the habitat through the sensitive olfactory system to carry out foraging, mating, oviposition, and other activities. The antennae serve as the primary olfactory organs in insects. The olfactory process involves...
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2025-02-01
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| author | Mengke Zhang Sumei Zhao Zhiping Xue Jiaying Sun Jiangning Hao Fengzhi Deng Junxia Huang Caroline Du Yongjun Du |
| author_facet | Mengke Zhang Sumei Zhao Zhiping Xue Jiaying Sun Jiangning Hao Fengzhi Deng Junxia Huang Caroline Du Yongjun Du |
| author_sort | Mengke Zhang |
| collection | DOAJ |
| description | Insects sense intraspecific or interspecific information about the chemical substances in the habitat through the sensitive olfactory system to carry out foraging, mating, oviposition, and other activities. The antennae serve as the primary olfactory organs in insects. The olfactory process involves the participation of many proteins, such as odorant-binding proteins (OBPs) and odorant receptors (ORs), but ORs play a central role in olfactory specificity and sensitivity. The beet webworm, <i>Loxostege sticticalis,</i> is an omnivorous agricultural pest that endangers crops and poses a significant risk to the agricultural and animal husbandry production in northern China. In this study, Illumina sequencing was conducted on the antennal transcriptome of male <i>L. sticticalis</i> trapped by three different sex pheromone blends. A total of 10,320 DEGs were identified, from which 46 candidate olfactory genes were selected for further analysis. These candidate olfactory genes comprise 13 odorant receptors, 6 ionotropic receptors (IRs), 3 gustatory receptors (GRs), 12 odorant-binding proteins, and 13 chemosensory proteins (CSPs). In summary, we analyzed the antennal transcriptome of male <i>L. sticticalis</i> trapped by three different sex pheromone blends and identified several candidate olfactory genes. This discovery offers a foundation for further molecular-level investigations into the olfactory system of <i>L. sticticalis</i>. |
| format | Article |
| id | doaj-art-2cb1327a74ed4e5f8b0c08099e2e5678 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-2cb1327a74ed4e5f8b0c08099e2e56782025-08-20T02:45:00ZengMDPI AGInsects2075-44502025-02-0116215210.3390/insects16020152Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone BlendsMengke Zhang0Sumei Zhao1Zhiping Xue2Jiaying Sun3Jiangning Hao4Fengzhi Deng5Junxia Huang6Caroline Du7Yongjun Du8Institute of Pesticides and Environmental Toxicology, Zhejiang University, Hangzhou 310058, ChinaInner Mongolia Autonomous Region Plant Protection and Quarantine Center, Hohhot 010010, ChinaBaotou Agricultural and Animal Husbandry Science Research Institute, Baotou 014030, ChinaInner Mongolia Autonomous Region Plant Protection and Quarantine Center, Hohhot 010010, ChinaInner Mongolia Autonomous Region Plant Protection and Quarantine Center, Hohhot 010010, ChinaInner Mongolia Autonomous Region Plant Protection and Quarantine Center, Hohhot 010010, ChinaInner Mongolia Autonomous Region Plant Protection and Quarantine Center, Hohhot 010010, ChinaIspxtech Inc., Hangzhou 310018, ChinaInstitute of Pesticides and Environmental Toxicology, Zhejiang University, Hangzhou 310058, ChinaInsects sense intraspecific or interspecific information about the chemical substances in the habitat through the sensitive olfactory system to carry out foraging, mating, oviposition, and other activities. The antennae serve as the primary olfactory organs in insects. The olfactory process involves the participation of many proteins, such as odorant-binding proteins (OBPs) and odorant receptors (ORs), but ORs play a central role in olfactory specificity and sensitivity. The beet webworm, <i>Loxostege sticticalis,</i> is an omnivorous agricultural pest that endangers crops and poses a significant risk to the agricultural and animal husbandry production in northern China. In this study, Illumina sequencing was conducted on the antennal transcriptome of male <i>L. sticticalis</i> trapped by three different sex pheromone blends. A total of 10,320 DEGs were identified, from which 46 candidate olfactory genes were selected for further analysis. These candidate olfactory genes comprise 13 odorant receptors, 6 ionotropic receptors (IRs), 3 gustatory receptors (GRs), 12 odorant-binding proteins, and 13 chemosensory proteins (CSPs). In summary, we analyzed the antennal transcriptome of male <i>L. sticticalis</i> trapped by three different sex pheromone blends and identified several candidate olfactory genes. This discovery offers a foundation for further molecular-level investigations into the olfactory system of <i>L. sticticalis</i>.https://www.mdpi.com/2075-4450/16/2/152<i>Loxostege sticticalis</i>olfactory systemolfactory genesantennal transcriptome |
| spellingShingle | Mengke Zhang Sumei Zhao Zhiping Xue Jiaying Sun Jiangning Hao Fengzhi Deng Junxia Huang Caroline Du Yongjun Du Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends Insects <i>Loxostege sticticalis</i> olfactory system olfactory genes antennal transcriptome |
| title | Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends |
| title_full | Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends |
| title_fullStr | Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends |
| title_full_unstemmed | Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends |
| title_short | Identification of Candidate Olfactory Genes in the Antennal Transcriptome of <i>Loxostege sticticalis</i> Trapped by Three Different Sex Pheromone Blends |
| title_sort | identification of candidate olfactory genes in the antennal transcriptome of i loxostege sticticalis i trapped by three different sex pheromone blends |
| topic | <i>Loxostege sticticalis</i> olfactory system olfactory genes antennal transcriptome |
| url | https://www.mdpi.com/2075-4450/16/2/152 |
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