Molecular characterization of a rice mitogen-activated protein kinase gene OsMPK4 and purification of recombinant protein and construction of binary vector
A rice gene, OsMPK4, encoding for mitogen-activated protein kinase, was cloned and characterized. The full-length cDNA of OsMPK4 consists of 1483 bp in size, containing an open reading frame of 1131 bp, which predicts to encode a protein of 376 amino acids with calculated molecular weight of 42.8 kD...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.06.0613 |
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| Summary: | A rice gene, OsMPK4, encoding for mitogen-activated protein kinase, was cloned and characterized. The full-length cDNA of OsMPK4 consists of 1483 bp in size, containing an open reading frame of 1131 bp, which predicts to encode a protein of 376 amino acids with calculated molecular weight of 42.8 kDa. The OsMPK4 gene is located on chromosome 10 of the rice genome and consists of 6 exons and 5 introns. The OsMPK4 protein contains all 11 conserved domains that are characteristics of MAPKs and phosphorylation site of TEY motif. Phylogenetic trees analysis revealed that OsMPK4 belongs to group B of the plant MAPKs; however, OsMPK4 shows a relatively low level of identity to all other characterized rice MAPKs, indicating that OsMPK4 is a new MAPK gene in rice. The recombinant OsMPK4 protein was purified from E. coli expressing the OsMPK4 gene and a binary vector used for rice transformation was also constructed, which will be helpful to elucidate the biochemical and biological functions of OsMPK4 in future studies. |
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| ISSN: | 1008-9209 2097-5155 |