Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation
Purpose. To describe morphology of lamellar hole-associated epiretinal proliferation (LHEP) removed from eyes with lamellar macular holes (LMH). Methods. Based on optical coherence tomography data, 10 specimens of LHEP were removed from 10 eyes with LMH during standard vitrectomy. Specimens were pre...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2015/450212 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849306715017707520 |
|---|---|
| author | Denise Compera Enrico Entchev Christos Haritoglou Wolfgang J. Mayer Felix Hagenau Jean Ziada Anselm Kampik Ricarda G. Schumann |
| author_facet | Denise Compera Enrico Entchev Christos Haritoglou Wolfgang J. Mayer Felix Hagenau Jean Ziada Anselm Kampik Ricarda G. Schumann |
| author_sort | Denise Compera |
| collection | DOAJ |
| description | Purpose. To describe morphology of lamellar hole-associated epiretinal proliferation (LHEP) removed from eyes with lamellar macular holes (LMH). Methods. Based on optical coherence tomography data, 10 specimens of LHEP were removed from 10 eyes with LMH during standard vitrectomy. Specimens were prepared for correlative light and electron microscopy (CLEM) using an immunonanogold particle of 1.4 nm diameter that was combined with a fluorescein moiety, both having been attached to a single antibody fragment. As primary antibodies, we used antiglial fibrillary acidic protein (GFAP), anti-CD45, anti-CD64, anti-α-smooth muscle actin (α-SMA), and anticollagen type I and type II. Results. In LHEP, GFAP-positive cells possess ultrastructural characteristics of fibroblasts and hyalocytes. They represent the major cell types and were densely packed in cell agglomerations on vitreous collagen strands. Epiretinal cells of LHEP rarely demonstrated contractive properties as α-SMA-positive myofibroblasts were an infrequent finding. Conclusion. CLEM indicates that epiretinal cells in LHEP might originate from the vitreous and that remodelling processes of vitreous collagen may play an important role in pathogenesis of eyes with LMH. |
| format | Article |
| id | doaj-art-2cad334bf64c4216ac28e94b0f081f0a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-2cad334bf64c4216ac28e94b0f081f0a2025-08-20T03:55:00ZengWileyJournal of Ophthalmology2090-004X2090-00582015-01-01201510.1155/2015/450212450212Correlative Microscopy of Lamellar Hole-Associated Epiretinal ProliferationDenise Compera0Enrico Entchev1Christos Haritoglou2Wolfgang J. Mayer3Felix Hagenau4Jean Ziada5Anselm Kampik6Ricarda G. Schumann7Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyDepartment of Ophthalmology, Ludwig-Maximilians-University, Mathildenstraße 8, 80336 Munich, GermanyPurpose. To describe morphology of lamellar hole-associated epiretinal proliferation (LHEP) removed from eyes with lamellar macular holes (LMH). Methods. Based on optical coherence tomography data, 10 specimens of LHEP were removed from 10 eyes with LMH during standard vitrectomy. Specimens were prepared for correlative light and electron microscopy (CLEM) using an immunonanogold particle of 1.4 nm diameter that was combined with a fluorescein moiety, both having been attached to a single antibody fragment. As primary antibodies, we used antiglial fibrillary acidic protein (GFAP), anti-CD45, anti-CD64, anti-α-smooth muscle actin (α-SMA), and anticollagen type I and type II. Results. In LHEP, GFAP-positive cells possess ultrastructural characteristics of fibroblasts and hyalocytes. They represent the major cell types and were densely packed in cell agglomerations on vitreous collagen strands. Epiretinal cells of LHEP rarely demonstrated contractive properties as α-SMA-positive myofibroblasts were an infrequent finding. Conclusion. CLEM indicates that epiretinal cells in LHEP might originate from the vitreous and that remodelling processes of vitreous collagen may play an important role in pathogenesis of eyes with LMH.http://dx.doi.org/10.1155/2015/450212 |
| spellingShingle | Denise Compera Enrico Entchev Christos Haritoglou Wolfgang J. Mayer Felix Hagenau Jean Ziada Anselm Kampik Ricarda G. Schumann Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation Journal of Ophthalmology |
| title | Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation |
| title_full | Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation |
| title_fullStr | Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation |
| title_full_unstemmed | Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation |
| title_short | Correlative Microscopy of Lamellar Hole-Associated Epiretinal Proliferation |
| title_sort | correlative microscopy of lamellar hole associated epiretinal proliferation |
| url | http://dx.doi.org/10.1155/2015/450212 |
| work_keys_str_mv | AT denisecompera correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT enricoentchev correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT christosharitoglou correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT wolfgangjmayer correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT felixhagenau correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT jeanziada correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT anselmkampik correlativemicroscopyoflamellarholeassociatedepiretinalproliferation AT ricardagschumann correlativemicroscopyoflamellarholeassociatedepiretinalproliferation |