Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides.
<h4>Background</h4>The cytoplasm of eukaryotic cells is a highly dynamic compartment where membranes readily undergo fission and fusion to reorganize the cytoplasmic architecture, and to import, export and transport various cargos within the cell. The double membrane of the nuclear envel...
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| Format: | Article |
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Public Library of Science (PLoS)
2009-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004255&type=printable |
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| author | Marie Garnier-Lhomme Richard D Byrne Tina M C Hobday Stephen Gschmeissner Rudiger Woscholski Dominic L Poccia Erick J Dufourc Banafshé Larijani |
| author_facet | Marie Garnier-Lhomme Richard D Byrne Tina M C Hobday Stephen Gschmeissner Rudiger Woscholski Dominic L Poccia Erick J Dufourc Banafshé Larijani |
| author_sort | Marie Garnier-Lhomme |
| collection | DOAJ |
| description | <h4>Background</h4>The cytoplasm of eukaryotic cells is a highly dynamic compartment where membranes readily undergo fission and fusion to reorganize the cytoplasmic architecture, and to import, export and transport various cargos within the cell. The double membrane of the nuclear envelope that surrounds the nucleus, segregates the chromosomes from cytoplasm and regulates nucleocytoplasmic transport through pores. Many details of its formation are still unclear. At fertilization the sperm devoid of nuclear envelope pores enters the egg. Although most of the sperm nuclear envelope disassembles, remnants of the envelope at the acrosomal and centriolar fossae do not and are subsequently incorporated into the newly forming male pronuclear envelope. Remnants are conserved from annelid to mammalian sperm.<h4>Methodology/principal findings</h4>Using lipid mass spectrometry and a new application of deuterium solid-state NMR spectroscopy we have characterized the lipid composition and membrane dynamics of the sperm nuclear envelope remnants in isolated sperm nuclei.<h4>Conclusions/significance</h4>We report nuclear envelope remnants are relatively fluid membranes rich in sterols, devoid of sphingomyelin, and highly enriched in polyphosphoinositides and polyunsaturated phospholipids. The localization of the polybasic effector domain of MARCKS illustrates the non-nuclear aspect of the polyphosphoinositides. Based on their atypical biophysical characteristics and phospholipid composition, we suggest a possible role for nuclear envelope remnants in membrane fusion leading to nuclear envelope assembly. |
| format | Article |
| id | doaj-art-2c9a01f3c39a403f8f5a09b3c91a4cbe |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-2c9a01f3c39a403f8f5a09b3c91a4cbe2025-08-20T02:00:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0141e425510.1371/journal.pone.0004255Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides.Marie Garnier-LhommeRichard D ByrneTina M C HobdayStephen GschmeissnerRudiger WoscholskiDominic L PocciaErick J DufourcBanafshé Larijani<h4>Background</h4>The cytoplasm of eukaryotic cells is a highly dynamic compartment where membranes readily undergo fission and fusion to reorganize the cytoplasmic architecture, and to import, export and transport various cargos within the cell. The double membrane of the nuclear envelope that surrounds the nucleus, segregates the chromosomes from cytoplasm and regulates nucleocytoplasmic transport through pores. Many details of its formation are still unclear. At fertilization the sperm devoid of nuclear envelope pores enters the egg. Although most of the sperm nuclear envelope disassembles, remnants of the envelope at the acrosomal and centriolar fossae do not and are subsequently incorporated into the newly forming male pronuclear envelope. Remnants are conserved from annelid to mammalian sperm.<h4>Methodology/principal findings</h4>Using lipid mass spectrometry and a new application of deuterium solid-state NMR spectroscopy we have characterized the lipid composition and membrane dynamics of the sperm nuclear envelope remnants in isolated sperm nuclei.<h4>Conclusions/significance</h4>We report nuclear envelope remnants are relatively fluid membranes rich in sterols, devoid of sphingomyelin, and highly enriched in polyphosphoinositides and polyunsaturated phospholipids. The localization of the polybasic effector domain of MARCKS illustrates the non-nuclear aspect of the polyphosphoinositides. Based on their atypical biophysical characteristics and phospholipid composition, we suggest a possible role for nuclear envelope remnants in membrane fusion leading to nuclear envelope assembly.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004255&type=printable |
| spellingShingle | Marie Garnier-Lhomme Richard D Byrne Tina M C Hobday Stephen Gschmeissner Rudiger Woscholski Dominic L Poccia Erick J Dufourc Banafshé Larijani Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides. PLoS ONE |
| title | Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides. |
| title_full | Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides. |
| title_fullStr | Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides. |
| title_full_unstemmed | Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides. |
| title_short | Nuclear envelope remnants: fluid membranes enriched in sterols and polyphosphoinositides. |
| title_sort | nuclear envelope remnants fluid membranes enriched in sterols and polyphosphoinositides |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0004255&type=printable |
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