Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component
Background. Central nervous system hemangioblastomas (HBs) are uncommon highly vascularized tumors that are predominantly found in the cerebellum. They occur sporadically or in association with von Hippel-Lindau (VHL) disease. HBs are of unknown histogenesis, and the origin of stromal cells is still...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2004-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500403273T.pdf |
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| author | Tasić Desanka Dimov Dragan Kostov Miloš Ilić Srbislav Dojčinov Dojčin Jovanović Milan Stanojević Goran Z. Dimov Irena |
| author_facet | Tasić Desanka Dimov Dragan Kostov Miloš Ilić Srbislav Dojčinov Dojčin Jovanović Milan Stanojević Goran Z. Dimov Irena |
| author_sort | Tasić Desanka |
| collection | DOAJ |
| description | Background. Central nervous system hemangioblastomas (HBs) are uncommon highly vascularized tumors that are predominantly found in the cerebellum. They occur sporadically or in association with von Hippel-Lindau (VHL) disease. HBs are of unknown histogenesis, and the origin of stromal cells is still a subject of debate. The aim of this study was to investigate the immunoprofile of neoplastic stromal component, and to determine whether the profile of the expression of immunomarkers used can contribute to the elucidation of the histogenesis of HBs. Methods. A series of eight cerebellar HBs were histochemically examined for the detection of mast cells and immunohistochemically for the expression of factor VIII-related antigen (FVIII-RAg), CD34, vimentin, factor XIIIa (FXIIIa), S-100 protein, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE) neurofilaments (NF), synaptophysin, chromogranin, and somatostatin. Results. Mast cells were present in all hemangioblastomas, and were particularly abundant in one tumor. Immunohistochemically, intense reactivity for vimentin and NSE in the stromal cells was constantly seen. Immunoreactivity with S-100 protein and FXIIIa was variable, but generally many HBs stromal cells were negative for these markers. However, stromal cells were uniformly negative for FVIII-RAg in all HBs investigated. They were negative for CD34 GFAP, NF, synaptophysin, chromogranin, as well as somatostatin. GFAP-positivity of the occasional stromal type cells, located only peripherally, was interpreted as "pseudopositivity". Conclusion. The immunoprofile of neoplastic stromal component in this study suggested a possible origin from undifferentiated multipotential mesenchymal cells. High expression of NSE (glycolytic and hypoxia-inducible enzyme) in the HBs stromal cells might be related to the loss of the VHL protein function. |
| format | Article |
| id | doaj-art-2c9377d661784ec6972eb1101774431e |
| institution | Kabale University |
| issn | 0042-8450 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-2c9377d661784ec6972eb1101774431e2025-08-20T03:55:00ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502004-01-0161327328210.2298/VSP0403273TCerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal componentTasić DesankaDimov DraganKostov MilošIlić SrbislavDojčinov DojčinJovanović MilanStanojević Goran Z.Dimov IrenaBackground. Central nervous system hemangioblastomas (HBs) are uncommon highly vascularized tumors that are predominantly found in the cerebellum. They occur sporadically or in association with von Hippel-Lindau (VHL) disease. HBs are of unknown histogenesis, and the origin of stromal cells is still a subject of debate. The aim of this study was to investigate the immunoprofile of neoplastic stromal component, and to determine whether the profile of the expression of immunomarkers used can contribute to the elucidation of the histogenesis of HBs. Methods. A series of eight cerebellar HBs were histochemically examined for the detection of mast cells and immunohistochemically for the expression of factor VIII-related antigen (FVIII-RAg), CD34, vimentin, factor XIIIa (FXIIIa), S-100 protein, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE) neurofilaments (NF), synaptophysin, chromogranin, and somatostatin. Results. Mast cells were present in all hemangioblastomas, and were particularly abundant in one tumor. Immunohistochemically, intense reactivity for vimentin and NSE in the stromal cells was constantly seen. Immunoreactivity with S-100 protein and FXIIIa was variable, but generally many HBs stromal cells were negative for these markers. However, stromal cells were uniformly negative for FVIII-RAg in all HBs investigated. They were negative for CD34 GFAP, NF, synaptophysin, chromogranin, as well as somatostatin. GFAP-positivity of the occasional stromal type cells, located only peripherally, was interpreted as "pseudopositivity". Conclusion. The immunoprofile of neoplastic stromal component in this study suggested a possible origin from undifferentiated multipotential mesenchymal cells. High expression of NSE (glycolytic and hypoxia-inducible enzyme) in the HBs stromal cells might be related to the loss of the VHL protein function.http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500403273T.pdfcerebellar neoplasmshemangioblastomastaining and labelinghistocytochemistryimmunohistochemistrystromal cellsimmunologic tests |
| spellingShingle | Tasić Desanka Dimov Dragan Kostov Miloš Ilić Srbislav Dojčinov Dojčin Jovanović Milan Stanojević Goran Z. Dimov Irena Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component Vojnosanitetski Pregled cerebellar neoplasms hemangioblastoma staining and labeling histocytochemistry immunohistochemistry stromal cells immunologic tests |
| title | Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component |
| title_full | Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component |
| title_fullStr | Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component |
| title_full_unstemmed | Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component |
| title_short | Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component |
| title_sort | cerebellar hemangioblastomas a study of the immunoprofile of neoplastic stromal component |
| topic | cerebellar neoplasms hemangioblastoma staining and labeling histocytochemistry immunohistochemistry stromal cells immunologic tests |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2004/0042-84500403273T.pdf |
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