Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition
Placozoa are small disc-shaped animals representing one of the early branching metazoan lineages with only a dozen cell types, fast effector reactions, and complex behaviors. The simplest organization and small cell sizes limit standard microscopy applications. Here, we implement a new methodology a...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-03-01
|
| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1531729/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850240471625891840 |
|---|---|
| author | Daria Y. Romanova Yana A. Frank Leonid L. Moroz Leonid L. Moroz |
| author_facet | Daria Y. Romanova Yana A. Frank Leonid L. Moroz Leonid L. Moroz |
| author_sort | Daria Y. Romanova |
| collection | DOAJ |
| description | Placozoa are small disc-shaped animals representing one of the early branching metazoan lineages with only a dozen cell types, fast effector reactions, and complex behaviors. The simplest organization and small cell sizes limit standard microscopy applications. Here, we implement a new methodology and protocol for expansion microscopy, improving both the resolution and preservation of fragile placozoans and kin. As a result, the proposed approaches can be applicable to a diversity of microscopic animals and their larvae with broad applicability for both laboratory and cross-disciplinary field research during long-term marine expeditions. |
| format | Article |
| id | doaj-art-2c935bdda4da4612979bdbae11293c31 |
| institution | OA Journals |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj-art-2c935bdda4da4612979bdbae11293c312025-08-20T02:00:51ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-03-011210.3389/fmars.2025.15317291531729Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expeditionDaria Y. Romanova0Yana A. Frank1Leonid L. Moroz2Leonid L. Moroz3Institute of Higher Nervous Activity and Neurophysiology of RAS, Moscow, RussiaInstitute of Higher Nervous Activity and Neurophysiology of RAS, Moscow, RussiaDepartments of Neuroscience and McKnight Brain Institute, University of Florida, Gainesville, FL, United StatesWhitney Laboratory for Marine Biosciences, University of Florida, St. Augustine, FL, United StatesPlacozoa are small disc-shaped animals representing one of the early branching metazoan lineages with only a dozen cell types, fast effector reactions, and complex behaviors. The simplest organization and small cell sizes limit standard microscopy applications. Here, we implement a new methodology and protocol for expansion microscopy, improving both the resolution and preservation of fragile placozoans and kin. As a result, the proposed approaches can be applicable to a diversity of microscopic animals and their larvae with broad applicability for both laboratory and cross-disciplinary field research during long-term marine expeditions.https://www.frontiersin.org/articles/10.3389/fmars.2025.1531729/fullplacozoactenophoratrichoplaxexpansion microscopymarine biologybiodiversity |
| spellingShingle | Daria Y. Romanova Yana A. Frank Leonid L. Moroz Leonid L. Moroz Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition Frontiers in Marine Science placozoa ctenophora trichoplax expansion microscopy marine biology biodiversity |
| title | Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition |
| title_full | Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition |
| title_fullStr | Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition |
| title_full_unstemmed | Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition |
| title_short | Expansion microscopy in Placozoa: improving resolution and preservation of fragile samples during marine expedition |
| title_sort | expansion microscopy in placozoa improving resolution and preservation of fragile samples during marine expedition |
| topic | placozoa ctenophora trichoplax expansion microscopy marine biology biodiversity |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1531729/full |
| work_keys_str_mv | AT dariayromanova expansionmicroscopyinplacozoaimprovingresolutionandpreservationoffragilesamplesduringmarineexpedition AT yanaafrank expansionmicroscopyinplacozoaimprovingresolutionandpreservationoffragilesamplesduringmarineexpedition AT leonidlmoroz expansionmicroscopyinplacozoaimprovingresolutionandpreservationoffragilesamplesduringmarineexpedition AT leonidlmoroz expansionmicroscopyinplacozoaimprovingresolutionandpreservationoffragilesamplesduringmarineexpedition |