Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i>
Foxtail millet (<i>Setaria italica</i>), a significant C4 model crop known for its exceptional photosynthetic efficiency and robust environmental adaptability, serves as an excellent model for investigating C4 photosynthesis and crop stress resilience. When subjected to abiotic stress, f...
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2025-06-01
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| author | Dan Zhu Xiaobing Hu Hailong Wang Yonghu Zhang Xianglong Li Wenqing Song Rui Wen Feng Feng Ran Chai Jianhua Wei Jiewei Zhang |
| author_facet | Dan Zhu Xiaobing Hu Hailong Wang Yonghu Zhang Xianglong Li Wenqing Song Rui Wen Feng Feng Ran Chai Jianhua Wei Jiewei Zhang |
| author_sort | Dan Zhu |
| collection | DOAJ |
| description | Foxtail millet (<i>Setaria italica</i>), a significant C4 model crop known for its exceptional photosynthetic efficiency and robust environmental adaptability, serves as an excellent model for investigating C4 photosynthesis and crop stress resilience. When subjected to abiotic stress, foxtail millet employs a sophisticated signal transduction network to regulate its physiological processes, ensuring sustained high photosynthetic efficiency and normal growth. The mitogen-activated protein kinase (MAPK) family plays a key role in plant growth, development, and stress response. Here, we identified and named a MAPK in <i>S. italica</i> as SiMPK6. Fluorescence quantitative PCR analysis revealed that SiMPK6 is mainly expressed in the leaves during the early shooting stage, with induction under various abiotic stresses such as low temperature, high osmotic pressure, high salt, high temperature, and high light. Overexpressing the <i>SiMPK6</i> in <i>Arabidopsis thaliana</i> mitigated damage to photosystem II induced by stress, underscoring the gene’s crucial role in foxtail millet’s stress signal transduction and maintenance of high photosynthetic efficiency. |
| format | Article |
| id | doaj-art-2c873df8f28d460091a8ee04e674ae5f |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-2c873df8f28d460091a8ee04e674ae5f2025-08-20T03:50:17ZengMDPI AGPlants2223-77472025-06-011413196010.3390/plants14131960Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i>Dan Zhu0Xiaobing Hu1Hailong Wang2Yonghu Zhang3Xianglong Li4Wenqing Song5Rui Wen6Feng Feng7Ran Chai8Jianhua Wei9Jiewei Zhang10School of Environmental Engineering, Yellow River Conservancy Technical University, Kaifeng 475004, ChinaSchool of Environmental Engineering, Yellow River Conservancy Technical University, Kaifeng 475004, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Crop Sciences, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Crop Sciences, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031, ChinaSchool of Environmental Engineering, Yellow River Conservancy Technical University, Kaifeng 475004, ChinaSchool of Environmental Engineering, Yellow River Conservancy Technical University, Kaifeng 475004, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaFoxtail millet (<i>Setaria italica</i>), a significant C4 model crop known for its exceptional photosynthetic efficiency and robust environmental adaptability, serves as an excellent model for investigating C4 photosynthesis and crop stress resilience. When subjected to abiotic stress, foxtail millet employs a sophisticated signal transduction network to regulate its physiological processes, ensuring sustained high photosynthetic efficiency and normal growth. The mitogen-activated protein kinase (MAPK) family plays a key role in plant growth, development, and stress response. Here, we identified and named a MAPK in <i>S. italica</i> as SiMPK6. Fluorescence quantitative PCR analysis revealed that SiMPK6 is mainly expressed in the leaves during the early shooting stage, with induction under various abiotic stresses such as low temperature, high osmotic pressure, high salt, high temperature, and high light. Overexpressing the <i>SiMPK6</i> in <i>Arabidopsis thaliana</i> mitigated damage to photosystem II induced by stress, underscoring the gene’s crucial role in foxtail millet’s stress signal transduction and maintenance of high photosynthetic efficiency.https://www.mdpi.com/2223-7747/14/13/1960<i>Setaria italica</i>MAPK proteinSiMPK6foxtail milletabiotic stressesphotosynthetic efficiency |
| spellingShingle | Dan Zhu Xiaobing Hu Hailong Wang Yonghu Zhang Xianglong Li Wenqing Song Rui Wen Feng Feng Ran Chai Jianhua Wei Jiewei Zhang Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i> Plants <i>Setaria italica</i> MAPK protein SiMPK6 foxtail millet abiotic stresses photosynthetic efficiency |
| title | Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i> |
| title_full | Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i> |
| title_fullStr | Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i> |
| title_full_unstemmed | Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i> |
| title_short | Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in <i>Setaria italica</i> |
| title_sort | insight into the functional role of simpk6 in stress response and photosynthetic efficiency in i setaria italica i |
| topic | <i>Setaria italica</i> MAPK protein SiMPK6 foxtail millet abiotic stresses photosynthetic efficiency |
| url | https://www.mdpi.com/2223-7747/14/13/1960 |
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