Refined DNA repair manipulation enables a universal knock-in strategy in mouse embryos

Refined DNA repair manipulation enables a universal knock-in strategy in mouse embryos

Abstract The design and screening of sgRNA in CRISPR-dependent gene knock-in is always laborious. Therefore, a universal and highly efficient knock-in strategy suitable for different sgRNA target sites is necessary. In our mouse embryo study, we find that the knock-in efficiency guided by adjacent s...

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Bibliographic Details
Main Authors: Hongyu Chen, Qingtong Tan, Li Li, Lanxin Li, Jiqiang Fu, Wencheng Zhu, Jie Li, Yining Wang, Shiyan Li, Huimin Li, Yidi Sun, Qiang Sun, Zongyang Lu, Zhen Liu
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61696-z
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