Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches
Graphical Abstract Highlight Research • The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. • Computational methods identified and optimized B-cell and T-cell e...
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Faculty of Fisheries and Marine Universitas Airlangga
2024-10-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/62035 |
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| author | Rozi Wiwiek Tyasningsih Jola Rahmahani Eduardus Bimo Aksono Muchammad Yunus Mohammad Anam Al-Arif Suryo Kuncorojati Rahayu Kusdarwati Putri Desi Wulan Sari Mohammad Noor Azmai Amal Annas Salleh Nadeem Khanand Suwarno |
| author_facet | Rozi Wiwiek Tyasningsih Jola Rahmahani Eduardus Bimo Aksono Muchammad Yunus Mohammad Anam Al-Arif Suryo Kuncorojati Rahayu Kusdarwati Putri Desi Wulan Sari Mohammad Noor Azmai Amal Annas Salleh Nadeem Khanand Suwarno |
| author_sort | Rozi |
| collection | DOAJ |
| description | Graphical Abstract
Highlight Research
• The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans.
• Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity.
• In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity.
• Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation.
• The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines.
Abstract
Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate. |
| format | Article |
| id | doaj-art-2c7ae513e4144f5aa2956d7bacea5508 |
| institution | DOAJ |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-2c7ae513e4144f5aa2956d7bacea55082025-08-20T03:20:25ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592024-10-0116229832160202Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico ApproachesRozi0https://orcid.org/0000-0003-4117-1335Wiwiek Tyasningsih1https://orcid.org/0000-0003-0604-8534Jola Rahmahani2https://orcid.org/0000-0001-9103-3200Eduardus Bimo Aksono3https://orcid.org/0000-0001-6961-2556Muchammad Yunus4https://orcid.org/0000-0001-7516-6628Mohammad Anam Al-Arif5https://orcid.org/0000-0002-5220-9311Suryo Kuncorojati6https://orcid.org/0000-0001-5170-9982Rahayu Kusdarwati7https://orcid.org/0000-0001-6281-6177Putri Desi Wulan Sari8https://orcid.org/0000-0001-8832-113XMohammad Noor Azmai Amal9https://orcid.org/0000-0002-7664-9821Annas Salleh10https://orcid.org/0000-0003-4142-0418Nadeem Khanand11Suwarno12https://orcid.org/0000-0002-4997-5242Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia Division of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia Division of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia Division of Farm, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, IndonesiaDivision of Farm, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, IndonesiaDivision of Veterinary Anatomy, Faculty of Veterinary Medicine, Airlangga University 60115, Surabaya, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine, Airlangga University, Surabaya 60115, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine, Airlangga University, Surabaya 60115, Indonesia Department of Biology, Faculty of Science, Universiti Putra Malaysia, MalaysiaDepartment of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia10Department of Bioinformatics, School of Interdisciplinary Engineering and Sciences, National University of Science and Technology, Islamabad, Pakistan.Division of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia Graphical Abstract Highlight Research • The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. • Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. • In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. • Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. • The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.https://e-journal.unair.ac.id/JIPK/article/view/62035immunoinformaticsmulti-epitopesmolecular docking |
| spellingShingle | Rozi Wiwiek Tyasningsih Jola Rahmahani Eduardus Bimo Aksono Muchammad Yunus Mohammad Anam Al-Arif Suryo Kuncorojati Rahayu Kusdarwati Putri Desi Wulan Sari Mohammad Noor Azmai Amal Annas Salleh Nadeem Khanand Suwarno Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Jurnal Ilmiah Perikanan dan Kelautan immunoinformatics multi-epitopes molecular docking |
| title | Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches |
| title_full | Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches |
| title_fullStr | Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches |
| title_full_unstemmed | Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches |
| title_short | Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches |
| title_sort | designing of a novel aerolysin based multiepitope vaccine against aeromonas hydrophila isolated from osphronemus goramy using reverse vaccinology an in silico approaches |
| topic | immunoinformatics multi-epitopes molecular docking |
| url | https://e-journal.unair.ac.id/JIPK/article/view/62035 |
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