Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure
Background: Epilepcy is a chronic neurological disease, and due to its complex mechanism, the current therapeutic drugs for it are not effective enough. It may have a non-neurological origin such as astrocytes and microglia. Objective This study aims to investigate the effect of cabergoline and leve...
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Guilan University of Medical Sciences
2023-04-01
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| Series: | مجله دانشگاه علوم پزشکی گیلان |
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| Online Access: | http://journal.gums.ac.ir/article-1-2573-en.pdf |
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| author | Mohammad Hossein Hajati Pishvari Yousef Panahi Gholamreza Hamidian |
| author_facet | Mohammad Hossein Hajati Pishvari Yousef Panahi Gholamreza Hamidian |
| author_sort | Mohammad Hossein Hajati Pishvari |
| collection | DOAJ |
| description | Background: Epilepcy is a chronic neurological disease, and due to its complex mechanism, the current therapeutic drugs for it are not effective enough. It may have a non-neurological origin such as astrocytes and microglia.
Objective This study aims to investigate the effect of cabergoline and levetiracetam (alone or combined) on the histological and stereological structure of the cerebral cortex, hippocampus, and cerebellum in rats with pentylenetetrazol (PTZ)-induced seizure.
Methods: In this experimental study, samples were 30 female rats in five groups of control, seizure (PTZ-induced kindling), seizure+levetiracetam, seizure+cabergoline, seizure+levetiracetam+cabergoline. Levetiracetam and cabergoline were used at 50 and 0.05 mg/kg doses, respectively, and half of these doses were used in the seizure+levetiracetam+cabergoline group. After anesthesia, animals’ brain tissue was removed and after preparing tissue slices, the number of neurons and neuroglia was examined using stereology technique.
Results: In the cerebral cortex and in the molecular and granular layers of the cerebellum, the numbers of neurons and neuroglia in the treatment groups were not significantly different from those in the control group, but a significant decrease was observed in the CA1, CA2, and CA3 regions of the hippocampus in the seizure group compared to the control group. In dentate gyrus, the number of neurons in all treatment groups and the number of neuroglia in the seizure group showed a significant decrease compared to the control group. In the Purkinje layer of the cerebellum, there was no significant change in the number of neurons compared to that in the control group.
Conclusion: The hippocampus is more involved in the occurrence of seizure activity than other parts of the brain. Neurons and neuroglia play an essential role in seizures, but more studies are needed for determining the relationship between the number of these cells and the use of cabergoline because their number decreases in different parts of the hippocampus following chronic seizures, where the relationship is different between dendrite gyrus and CA regions. |
| format | Article |
| id | doaj-art-2c78e09971b04af2b6f87fa0e51354ae |
| institution | DOAJ |
| issn | 2008-4048 2008-4056 |
| language | fas |
| publishDate | 2023-04-01 |
| publisher | Guilan University of Medical Sciences |
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| series | مجله دانشگاه علوم پزشکی گیلان |
| spelling | doaj-art-2c78e09971b04af2b6f87fa0e51354ae2025-08-20T03:04:59ZfasGuilan University of Medical Sciencesمجله دانشگاه علوم پزشکی گیلان2008-40482008-40562023-04-013211829Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced SeizureMohammad Hossein Hajati Pishvari0Yousef Panahi1Gholamreza Hamidian2 Division of Pharmacology and Toxicology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. Division of Pharmacology and Toxicology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. Division of Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. Background: Epilepcy is a chronic neurological disease, and due to its complex mechanism, the current therapeutic drugs for it are not effective enough. It may have a non-neurological origin such as astrocytes and microglia. Objective This study aims to investigate the effect of cabergoline and levetiracetam (alone or combined) on the histological and stereological structure of the cerebral cortex, hippocampus, and cerebellum in rats with pentylenetetrazol (PTZ)-induced seizure. Methods: In this experimental study, samples were 30 female rats in five groups of control, seizure (PTZ-induced kindling), seizure+levetiracetam, seizure+cabergoline, seizure+levetiracetam+cabergoline. Levetiracetam and cabergoline were used at 50 and 0.05 mg/kg doses, respectively, and half of these doses were used in the seizure+levetiracetam+cabergoline group. After anesthesia, animals’ brain tissue was removed and after preparing tissue slices, the number of neurons and neuroglia was examined using stereology technique. Results: In the cerebral cortex and in the molecular and granular layers of the cerebellum, the numbers of neurons and neuroglia in the treatment groups were not significantly different from those in the control group, but a significant decrease was observed in the CA1, CA2, and CA3 regions of the hippocampus in the seizure group compared to the control group. In dentate gyrus, the number of neurons in all treatment groups and the number of neuroglia in the seizure group showed a significant decrease compared to the control group. In the Purkinje layer of the cerebellum, there was no significant change in the number of neurons compared to that in the control group. Conclusion: The hippocampus is more involved in the occurrence of seizure activity than other parts of the brain. Neurons and neuroglia play an essential role in seizures, but more studies are needed for determining the relationship between the number of these cells and the use of cabergoline because their number decreases in different parts of the hippocampus following chronic seizures, where the relationship is different between dendrite gyrus and CA regions.http://journal.gums.ac.ir/article-1-2573-en.pdfepilepsyneuronneurogliastereologyprolactin. |
| spellingShingle | Mohammad Hossein Hajati Pishvari Yousef Panahi Gholamreza Hamidian Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure مجله دانشگاه علوم پزشکی گیلان epilepsy neuron neuroglia stereology prolactin. |
| title | Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure |
| title_full | Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure |
| title_fullStr | Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure |
| title_full_unstemmed | Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure |
| title_short | Effects of Cabergoline and Levetiracetam on the Histological and Stereological Structure of the Cerebral Cortex, Hippocampus and Cerebellum of Rats With Pentylenetetrazol-Induced Seizure |
| title_sort | effects of cabergoline and levetiracetam on the histological and stereological structure of the cerebral cortex hippocampus and cerebellum of rats with pentylenetetrazol induced seizure |
| topic | epilepsy neuron neuroglia stereology prolactin. |
| url | http://journal.gums.ac.ir/article-1-2573-en.pdf |
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