Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery
Objective. Obesity is associated with metabolic dysfunction with sex differences and chronic, low-grade inflammation. We proposed that hepatic expression of immune complement C3 related receptors (C3aR, C5aR, and C5L2) would be associated with pre- or postmenopausal status and metabolic profile in s...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Obesity |
| Online Access: | http://dx.doi.org/10.1155/2014/383102 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554623438684160 |
|---|---|
| author | Reza Rezvani Jessica Smith Marc Lapointe Picard Marceau Andre Tchernof Katherine Cianflone |
| author_facet | Reza Rezvani Jessica Smith Marc Lapointe Picard Marceau Andre Tchernof Katherine Cianflone |
| author_sort | Reza Rezvani |
| collection | DOAJ |
| description | Objective. Obesity is associated with metabolic dysfunction with sex differences and chronic, low-grade inflammation. We proposed that hepatic expression of immune complement C3 related receptors (C3aR, C5aR, and C5L2) would be associated with pre- or postmenopausal status and metabolic profile in severely obese women. We hypothesized that C5L2/C5aR ratio, potentially influencing the ASP/C5L2 metabolic versus C5a/C5aR immune response, would predict metabolic profiles after weight loss surgery. Materials and Methods. Fasting plasma (hormone, lipid, and enzyme analysis) and liver biopsies (RT-PCR gene expression) were obtained from 91 women during surgery. Results. Hepatic C5L2 mRNA expression was elevated in pre- versus postmenopausal women (P<0.01) and correlated positively with circulating estradiol, estrone, ApoB, ApoA1, ApoA1/B, waist circumference, age, and LDL-C (all P<0.05). While plasma ASP was lower in pre- versus postmenopausal women (P<0.01), the hepatic C5L2/C5aR mRNA ratio was increased (P<0.001) and correlated positively with estrone (P<0.01) and estradiol (P<0.001) and negatively with circulating ApoB and liver enzymes ALT, AST, and GGT (all P<0.05). Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P<0.05, AST and GGT, P<0.001 2-way-ANOVA). Conclusion. C5L2-C5aR association with other mediators including estrogens may contribute to hepatic metabolic and inflammatory function. |
| format | Article |
| id | doaj-art-2c73bdd2706e45d8bb43e1faf29ded0a |
| institution | Kabale University |
| issn | 2090-0708 2090-0716 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Obesity |
| spelling | doaj-art-2c73bdd2706e45d8bb43e1faf29ded0a2025-02-03T05:50:54ZengWileyJournal of Obesity2090-07082090-07162014-01-01201410.1155/2014/383102383102Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric SurgeryReza Rezvani0Jessica Smith1Marc Lapointe2Picard Marceau3Andre Tchernof4Katherine Cianflone5Centre de Recherche de l’Institut Universitaire de Cardiologie & Pneumologie de Québec, Université Laval, Y4332, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie & Pneumologie de Québec, Université Laval, Y4332, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie & Pneumologie de Québec, Université Laval, Y4332, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie & Pneumologie de Québec, Université Laval, Y4332, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie & Pneumologie de Québec, Université Laval, Y4332, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie & Pneumologie de Québec, Université Laval, Y4332, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaObjective. Obesity is associated with metabolic dysfunction with sex differences and chronic, low-grade inflammation. We proposed that hepatic expression of immune complement C3 related receptors (C3aR, C5aR, and C5L2) would be associated with pre- or postmenopausal status and metabolic profile in severely obese women. We hypothesized that C5L2/C5aR ratio, potentially influencing the ASP/C5L2 metabolic versus C5a/C5aR immune response, would predict metabolic profiles after weight loss surgery. Materials and Methods. Fasting plasma (hormone, lipid, and enzyme analysis) and liver biopsies (RT-PCR gene expression) were obtained from 91 women during surgery. Results. Hepatic C5L2 mRNA expression was elevated in pre- versus postmenopausal women (P<0.01) and correlated positively with circulating estradiol, estrone, ApoB, ApoA1, ApoA1/B, waist circumference, age, and LDL-C (all P<0.05). While plasma ASP was lower in pre- versus postmenopausal women (P<0.01), the hepatic C5L2/C5aR mRNA ratio was increased (P<0.001) and correlated positively with estrone (P<0.01) and estradiol (P<0.001) and negatively with circulating ApoB and liver enzymes ALT, AST, and GGT (all P<0.05). Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P<0.05, AST and GGT, P<0.001 2-way-ANOVA). Conclusion. C5L2-C5aR association with other mediators including estrogens may contribute to hepatic metabolic and inflammatory function.http://dx.doi.org/10.1155/2014/383102 |
| spellingShingle | Reza Rezvani Jessica Smith Marc Lapointe Picard Marceau Andre Tchernof Katherine Cianflone Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery Journal of Obesity |
| title | Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery |
| title_full | Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery |
| title_fullStr | Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery |
| title_full_unstemmed | Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery |
| title_short | Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery |
| title_sort | complement receptors c5ar and c5l2 are associated with metabolic profile sex hormones and liver enzymes in obese women pre and postbariatric surgery |
| url | http://dx.doi.org/10.1155/2014/383102 |
| work_keys_str_mv | AT rezarezvani complementreceptorsc5arandc5l2areassociatedwithmetabolicprofilesexhormonesandliverenzymesinobesewomenpreandpostbariatricsurgery AT jessicasmith complementreceptorsc5arandc5l2areassociatedwithmetabolicprofilesexhormonesandliverenzymesinobesewomenpreandpostbariatricsurgery AT marclapointe complementreceptorsc5arandc5l2areassociatedwithmetabolicprofilesexhormonesandliverenzymesinobesewomenpreandpostbariatricsurgery AT picardmarceau complementreceptorsc5arandc5l2areassociatedwithmetabolicprofilesexhormonesandliverenzymesinobesewomenpreandpostbariatricsurgery AT andretchernof complementreceptorsc5arandc5l2areassociatedwithmetabolicprofilesexhormonesandliverenzymesinobesewomenpreandpostbariatricsurgery AT katherinecianflone complementreceptorsc5arandc5l2areassociatedwithmetabolicprofilesexhormonesandliverenzymesinobesewomenpreandpostbariatricsurgery |