Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines

Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines

Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5637 cells) in order to confirm the existence o...

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Main Authors: Ibtissam Youlyouz, Emmanuelle Magnoux, Laurence Guglielmi, Yves Denizot
Format: Article
Language:English
Published: Wiley 2002-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1080/09629350210000015755
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author Ibtissam Youlyouz
Emmanuelle Magnoux
Laurence Guglielmi
Yves Denizot
author_facet Ibtissam Youlyouz
Emmanuelle Magnoux
Laurence Guglielmi
Yves Denizot
author_sort Ibtissam Youlyouz
collection DOAJ
description Platelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5637 cells) in order to confirm the existence of a splice variant of the PAF-R transcript 2. After cloning and sequencing, we confirmed its existence in all cell lines. It consisted of the PAF-R transcript 2 lengthening with 82 nucleotides from the 3' end of exon 1 of the PAF-R gene. The role of this elongated form of the tissue-type PAF-R transcript in cell physiology remains to be elucidated.
format Article
id doaj-art-2c6d6ee7b1c2442cbe68c814630847c4
institution Kabale University
issn 0962-9351
1466-1861
language English
publishDate 2002-01-01
publisher Wiley
record_format Article
series Mediators of Inflammation
spelling doaj-art-2c6d6ee7b1c2442cbe68c814630847c42025-02-03T05:50:29ZengWileyMediators of Inflammation0962-93511466-18612002-01-0111532933110.1080/09629350210000015755Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell linesIbtissam Youlyouz0Emmanuelle Magnoux1Laurence Guglielmi2Yves Denizot3Laboratoire d'Immunologie – UMR CNRS 6101, Faculté de Médecine, 2 rue du Docteur Marcland, Limoges 87025, FranceLaboratoire d'Immunologie – UMR CNRS 6101, Faculté de Médecine, 2 rue du Docteur Marcland, Limoges 87025, FranceLaboratoire d'Immunologie – UMR CNRS 6101, Faculté de Médecine, 2 rue du Docteur Marcland, Limoges 87025, FranceLaboratoire d'Immunologie – UMR CNRS 6101, Faculté de Médecine, 2 rue du Docteur Marcland, Limoges 87025, FrancePlatelet-activating factor receptor (PAF-R) transcripts were analysed by reverse transcriptase-polymerase chain reaction in five human cancer cell lines derived from the breast (BT20, SKBR3 and T47D cells), the pancreas (Miapaca cells) and the bladder (5637 cells) in order to confirm the existence of a splice variant of the PAF-R transcript 2. After cloning and sequencing, we confirmed its existence in all cell lines. It consisted of the PAF-R transcript 2 lengthening with 82 nucleotides from the 3' end of exon 1 of the PAF-R gene. The role of this elongated form of the tissue-type PAF-R transcript in cell physiology remains to be elucidated.http://dx.doi.org/10.1080/09629350210000015755
spellingShingle Ibtissam Youlyouz
Emmanuelle Magnoux
Laurence Guglielmi
Yves Denizot
Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
Mediators of Inflammation
title Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
title_full Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
title_fullStr Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
title_full_unstemmed Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
title_short Expression of a splice variant of the platelet-activating factor receptor transcript 2 in various human cancer cell lines
title_sort expression of a splice variant of the platelet activating factor receptor transcript 2 in various human cancer cell lines
url http://dx.doi.org/10.1080/09629350210000015755
work_keys_str_mv AT ibtissamyoulyouz expressionofasplicevariantoftheplateletactivatingfactorreceptortranscript2invarioushumancancercelllines
AT emmanuellemagnoux expressionofasplicevariantoftheplateletactivatingfactorreceptortranscript2invarioushumancancercelllines
AT laurenceguglielmi expressionofasplicevariantoftheplateletactivatingfactorreceptortranscript2invarioushumancancercelllines
AT yvesdenizot expressionofasplicevariantoftheplateletactivatingfactorreceptortranscript2invarioushumancancercelllines

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