Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates
Pingjuan Liu,1,* Mengling Qin,2,* Chenfeng Zhao,1,* Siting Yi,3 Mengmin Ye,3 Kang Liao,1 Jiankai Deng,1 Yili Chen1 1Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People’s Republic of China; 2Departmen...
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Dove Medical Press
2025-03-01
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| author | Liu P Qin M Zhao C Yi S Ye M Liao K Deng J Chen Y |
| author_facet | Liu P Qin M Zhao C Yi S Ye M Liao K Deng J Chen Y |
| author_sort | Liu P |
| collection | DOAJ |
| description | Pingjuan Liu,1,* Mengling Qin,2,* Chenfeng Zhao,1,* Siting Yi,3 Mengmin Ye,3 Kang Liao,1 Jiankai Deng,1 Yili Chen1 1Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People’s Republic of China; 2Department of Laboratory Medicine, Hainan West Central Hospital, Hainan, 571700, People’s Republic of China; 3Department of Laboratory Medicine, The East Division of The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yili Chen; Jiankai Deng, Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510080, People’s Republic of China, Email chenyli3@mail.sysu.edu.cn; dengjk0417@126.comIntroduction: The rapid and accurate identification of carbapenemases in Enterobacterales isolates is of paramount importance for the selection of effective antibiotics and the control of hospital-acquired infections.Methods: This study aimed to evaluate the performance of two immunochromatographic methods, NG-Test Carba 5 (Carba 5) and Goldstream Carbapenem-resistant K.N.I.V.O. Detection K-Set (K-Set) for detecting five major carbapenemase (KPC, NDM, IMP, OXA-48-like, and VIM). Carbapenemase genes were confirmed by PCR.Results: In this study, a total of 245 carbapenem-resistant Enterobacterales (CRE) isolates were encompassed, with an overwhelming 96.7% of these strains exhibiting the ability to produce carbapenemase. A total of 58.2% of Klebsiella pneumoniae strains that produce KPC carbapenemase were the most prevalent among carbapenem-resistant Enterobacteriaceae (CRE). NDM-producing Klebsiella pneumoniae accounted for 30.4%. Importantly, NDM-type carbapenemase emerges as the predominant form in Escherichia coli and Enterobacter cloacae strains, accounting for 46 (93.9%) and 20 (83.3%) cases, respectively. The performance of the two methods in carbapenemase detection has demonstrated remarkable outcomes, exhibiting overall specificity and sensitivity exceeding 99%. Specifically, the K-Set accurately detected a unique KPC-carbapenemase in K. pneumoniae, whereas Carba 5 was unable to identify it. This was due to the presence of a novel blaKPC gene, which harbored a specific point mutation (A to G) at nucleotide position 787, differentiating it from the blaKPC-33 gene.Conclusion: These two methods, characterized by their simplicity, rapidity, and accuracy, are ideally suited for detecting carbapenemases in routine microbiology laboratories. They serve as a vital foundation for the rational selection of antibiotics in clinical practice.Keywords: carbapenem-resistant Enterobacterales, CRE, carbapenemase gene, rapid immunochromatographic |
| format | Article |
| id | doaj-art-2c6310c213fd46c386da9fe9f8e6bae2 |
| institution | DOAJ |
| issn | 1178-6973 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Dove Medical Press |
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| series | Infection and Drug Resistance |
| spelling | doaj-art-2c6310c213fd46c386da9fe9f8e6bae22025-08-20T02:57:13ZengDove Medical PressInfection and Drug Resistance1178-69732025-03-01Volume 1814151424101074Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical IsolatesLiu PQin MZhao CYi SYe MLiao KDeng JChen YPingjuan Liu,1,* Mengling Qin,2,* Chenfeng Zhao,1,* Siting Yi,3 Mengmin Ye,3 Kang Liao,1 Jiankai Deng,1 Yili Chen1 1Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People’s Republic of China; 2Department of Laboratory Medicine, Hainan West Central Hospital, Hainan, 571700, People’s Republic of China; 3Department of Laboratory Medicine, The East Division of The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, 510080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yili Chen; Jiankai Deng, Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510080, People’s Republic of China, Email chenyli3@mail.sysu.edu.cn; dengjk0417@126.comIntroduction: The rapid and accurate identification of carbapenemases in Enterobacterales isolates is of paramount importance for the selection of effective antibiotics and the control of hospital-acquired infections.Methods: This study aimed to evaluate the performance of two immunochromatographic methods, NG-Test Carba 5 (Carba 5) and Goldstream Carbapenem-resistant K.N.I.V.O. Detection K-Set (K-Set) for detecting five major carbapenemase (KPC, NDM, IMP, OXA-48-like, and VIM). Carbapenemase genes were confirmed by PCR.Results: In this study, a total of 245 carbapenem-resistant Enterobacterales (CRE) isolates were encompassed, with an overwhelming 96.7% of these strains exhibiting the ability to produce carbapenemase. A total of 58.2% of Klebsiella pneumoniae strains that produce KPC carbapenemase were the most prevalent among carbapenem-resistant Enterobacteriaceae (CRE). NDM-producing Klebsiella pneumoniae accounted for 30.4%. Importantly, NDM-type carbapenemase emerges as the predominant form in Escherichia coli and Enterobacter cloacae strains, accounting for 46 (93.9%) and 20 (83.3%) cases, respectively. The performance of the two methods in carbapenemase detection has demonstrated remarkable outcomes, exhibiting overall specificity and sensitivity exceeding 99%. Specifically, the K-Set accurately detected a unique KPC-carbapenemase in K. pneumoniae, whereas Carba 5 was unable to identify it. This was due to the presence of a novel blaKPC gene, which harbored a specific point mutation (A to G) at nucleotide position 787, differentiating it from the blaKPC-33 gene.Conclusion: These two methods, characterized by their simplicity, rapidity, and accuracy, are ideally suited for detecting carbapenemases in routine microbiology laboratories. They serve as a vital foundation for the rational selection of antibiotics in clinical practice.Keywords: carbapenem-resistant Enterobacterales, CRE, carbapenemase gene, rapid immunochromatographichttps://www.dovepress.com/evaluating-the-performance-of-two-rapid-immunochromatographic-techniqu-peer-reviewed-fulltext-article-IDRcarbapenem-resistant enterobacteralescrecarbapenemase generapid immunochromatographic |
| spellingShingle | Liu P Qin M Zhao C Yi S Ye M Liao K Deng J Chen Y Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates Infection and Drug Resistance carbapenem-resistant enterobacterales cre carbapenemase gene rapid immunochromatographic |
| title | Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates |
| title_full | Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates |
| title_fullStr | Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates |
| title_full_unstemmed | Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates |
| title_short | Evaluating the Performance of Two Rapid Immunochromatographic Techniques for Detecting Carbapenemase in Carbapenem-Resistant Enterobacterales Clinical Isolates |
| title_sort | evaluating the performance of two rapid immunochromatographic techniques for detecting carbapenemase in carbapenem resistant enterobacterales clinical isolates |
| topic | carbapenem-resistant enterobacterales cre carbapenemase gene rapid immunochromatographic |
| url | https://www.dovepress.com/evaluating-the-performance-of-two-rapid-immunochromatographic-techniqu-peer-reviewed-fulltext-article-IDR |
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