Tuina Mitigates Blood–Brain Barrier Disruption in Hypoxic–Ischemic Encephalopathy Rats by Inhibiting PARthanatos in Endothelial Cells
Objective: This study aimed to investigate the protective effects of Tuina on the blood–brain barrier (BBB) in hypoxic-ischemic encephalopathy (HIE) rats and evaluate the underlying mechanisms of endothelial PARthanatos attenuation. Materials and Methods: Male Sprague-Dawley (SD) rats were subjected...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2025-01-01
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| Series: | World Journal of Traditional Chinese Medicine |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/wjtcm.wjtcm_101_24 |
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| Summary: | Objective:
This study aimed to investigate the protective effects of Tuina on the blood–brain barrier (BBB) in hypoxic-ischemic encephalopathy (HIE) rats and evaluate the underlying mechanisms of endothelial PARthanatos attenuation.
Materials and Methods:
Male Sprague-Dawley (SD) rats were subjected to occlusion of the left common carotid artery and hypoxia-ischemia. Tuina treatment was performed once daily for 15 min. Body weight, righting reflex, and balance beam tests were conducted to evaluate the growth state. Evans blue (EB) staining and magnetic resonance imaging (MRI) were used to assess BBB permeability. Cortical cell morphology was analyzed using the electron microscopy and hematoxylin and eosin staining. Immunofluorescence co-staining revealed Poly (ADP-ribose) polymerase-1 (PARP1) hyperactivation in cortical endothelial cells. Tight junction and PARthanatos-related proteins were detected by western blotting.
Results:
Tuina significantly increased body weight and reduced righting reflex time in rats with HIE. Tuina-treated HIE rats exhibited fewer hind limb slips and required less time to cross the balance beam. The EB content in the brains of Tuina-treated HIE rats was significantly lower than in the brains of HIE rats. MRI showed that the intensity in Tuina-treated HIE rats was significantly lower than in untreated HIE rats. After HI injury, Tuina alleviated neuronal mitochondrial and ER damage and diminished CD31+/PARP1 + immunofluorescence in the cortex. In the Tuina + HIE group, the level of tight junction proteins increased, while PARP1 and apoptosis-inducing factors levels were reduced in the cortex compared to the HIE group.
Conclusions:
Tuina attenuated BBB disruption in rats with HIE by increasing the expression of tight junction proteins and reducing the expression of endothelial PARthanatos in the cortex. |
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| ISSN: | 2311-8571 |