The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics
Trametes lactinea mycelia polysaccharides (TLMPS) have a wide range of bioactivities. The potential mechanisms of action of TLMPS against acute alcohol-induced liver injury in vivo were investigated by analyzing the physical and chemical properties of TLMPS and its protective effects on a mouse mode...
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| Language: | English |
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Tsinghua University Press
2021-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000574 |
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| author | Jinbin Hao Liyun Ye Guoliang Meng Yanjiao Song Junsheng Fu Xiaoping Wu |
| author_facet | Jinbin Hao Liyun Ye Guoliang Meng Yanjiao Song Junsheng Fu Xiaoping Wu |
| author_sort | Jinbin Hao |
| collection | DOAJ |
| description | Trametes lactinea mycelia polysaccharides (TLMPS) have a wide range of bioactivities. The potential mechanisms of action of TLMPS against acute alcohol-induced liver injury in vivo were investigated by analyzing the physical and chemical properties of TLMPS and its protective effects on a mouse model of alcoholic liver injury. TLMPS protected the liver against alcohol-induced injury, as evidenced by the reduced alcohol-induced elevation of the liver index, serum biochemical indices, and maintenance of hepatic morphology. Potential mechanisms were analyzed using transcriptome and metabolome analyses. The transcriptome data revealed the involvement of many differentially expressed genes in chemical carcinogenesis, drug metabolism, and metabolism of xenobiotics by cytochrome P450. The metabolome analysis revealed that TLMPS significantly regulated specific metabolites in the liver, including organic acids, lipids, nucleosides, and organic oxygen compounds. KEGG enrichment analysis revealed the significant involvement of different metabolites in choline metabolism, ATP-binding cassette transporters, and glycerophospholipid metabolism. Assessment of the changes in gene expression and metabolites revealed the significantly different expression of several genes encoding key enzymes and metabolites in choline metabolism pathway. The collective findings confirmed that choline metabolism plays an important role in the protective effects of TLMPS against acute alcoholic liver injury. |
| format | Article |
| id | doaj-art-2c50d00003d8450ea0b0826d0f0a3894 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2c50d00003d8450ea0b0826d0f0a38942025-02-02T23:24:38ZengTsinghua University PressFood Science and Human Wellness2213-45302021-07-01104480489The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomicsJinbin Hao0Liyun Ye1Guoliang Meng2Yanjiao Song3Junsheng Fu4Xiaoping Wu5College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, ChinaCorresponding author.; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002 Fujian, ChinaTrametes lactinea mycelia polysaccharides (TLMPS) have a wide range of bioactivities. The potential mechanisms of action of TLMPS against acute alcohol-induced liver injury in vivo were investigated by analyzing the physical and chemical properties of TLMPS and its protective effects on a mouse model of alcoholic liver injury. TLMPS protected the liver against alcohol-induced injury, as evidenced by the reduced alcohol-induced elevation of the liver index, serum biochemical indices, and maintenance of hepatic morphology. Potential mechanisms were analyzed using transcriptome and metabolome analyses. The transcriptome data revealed the involvement of many differentially expressed genes in chemical carcinogenesis, drug metabolism, and metabolism of xenobiotics by cytochrome P450. The metabolome analysis revealed that TLMPS significantly regulated specific metabolites in the liver, including organic acids, lipids, nucleosides, and organic oxygen compounds. KEGG enrichment analysis revealed the significant involvement of different metabolites in choline metabolism, ATP-binding cassette transporters, and glycerophospholipid metabolism. Assessment of the changes in gene expression and metabolites revealed the significantly different expression of several genes encoding key enzymes and metabolites in choline metabolism pathway. The collective findings confirmed that choline metabolism plays an important role in the protective effects of TLMPS against acute alcoholic liver injury.http://www.sciencedirect.com/science/article/pii/S2213453021000574Trametes lactineaPolysaccharideHepatoprotectiveTranscriptomeMetabolome |
| spellingShingle | Jinbin Hao Liyun Ye Guoliang Meng Yanjiao Song Junsheng Fu Xiaoping Wu The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics Food Science and Human Wellness Trametes lactinea Polysaccharide Hepatoprotective Transcriptome Metabolome |
| title | The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics |
| title_full | The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics |
| title_fullStr | The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics |
| title_full_unstemmed | The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics |
| title_short | The protective effect and crucial biological pathways analysis of Trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics |
| title_sort | protective effect and crucial biological pathways analysis of trametes lactinea mycelium polysaccharides on acute alcoholic liver injury in mice based on transcriptomics and metabonomics |
| topic | Trametes lactinea Polysaccharide Hepatoprotective Transcriptome Metabolome |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000574 |
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