Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis)
The Plant peroxidases are remarkable enzymes due to their widespread use in industry. These enzymes, which are capable of catalyzing the oxidation of various organic and inorganic substrates, have been used in clinical diagnosis, detoxification reactions and organic synthesis. In this study, in vitr...
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Sakarya University
2021-10-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1461230 |
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| author | Şeyma Önlü Züleyha Almaz Aykut Öztekin |
| author_facet | Şeyma Önlü Züleyha Almaz Aykut Öztekin |
| author_sort | Şeyma Önlü |
| collection | DOAJ |
| description | The Plant peroxidases are remarkable enzymes due to their widespread use in industry. These enzymes, which are capable of catalyzing the oxidation of various organic and inorganic substrates, have been used in clinical diagnosis, detoxification reactions and organic synthesis. In this study, in vitro production and purification of peroxidase enzyme from cauliflower plant was proposed. Firstly, sterile seedlings were obtained from MS/B5 nutrient medium without growth regulator from cauliflower seeds and calluses from medium containing 0.5 mg / L 2.4-D. Callus and seedlings were powdered with liquid nitrogen and then homogenized. Peroxidase enzymes were purified from these homogenates using affinity technique. SDS-PAGE electrophoresis was performed to determine the molecular weight of the purified enzymes and single bands was observed at approximately 46 kDa. In addition, KM and Vmax values of the callus peroxidase enzyme were determined for guaiacol, pyrogallol and H2O2 substrates. |
| format | Article |
| id | doaj-art-2c467a8ae312488995f961d8236d6830 |
| institution | OA Journals |
| issn | 2147-835X |
| language | English |
| publishDate | 2021-10-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-2c467a8ae312488995f961d8236d68302025-08-20T02:31:40ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2021-10-012551121112810.16984/saufenbilder.84470528Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis)Şeyma Önlü0https://orcid.org/0000-0003-2005-1019Züleyha Almaz1https://orcid.org/0000-0002-4532-4311Aykut Öztekin2https://orcid.org/0000-0003-1418-179XFEN-EDEBİYAT FAKÜLTESİMUŞ ALPARSLAN ÜNİVERSİTESİATATÜRK ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, KİMYA (YL) (TEZLİ) (AĞRI İBRAHİM ÇEÇEN ÜNİV. ORTAK)The Plant peroxidases are remarkable enzymes due to their widespread use in industry. These enzymes, which are capable of catalyzing the oxidation of various organic and inorganic substrates, have been used in clinical diagnosis, detoxification reactions and organic synthesis. In this study, in vitro production and purification of peroxidase enzyme from cauliflower plant was proposed. Firstly, sterile seedlings were obtained from MS/B5 nutrient medium without growth regulator from cauliflower seeds and calluses from medium containing 0.5 mg / L 2.4-D. Callus and seedlings were powdered with liquid nitrogen and then homogenized. Peroxidase enzymes were purified from these homogenates using affinity technique. SDS-PAGE electrophoresis was performed to determine the molecular weight of the purified enzymes and single bands was observed at approximately 46 kDa. In addition, KM and Vmax values of the callus peroxidase enzyme were determined for guaiacol, pyrogallol and H2O2 substrates.https://dergipark.org.tr/tr/download/article-file/1461230cauliflower (brassica oleracea l. var. botrytis)plant tissue cultureperoxidase purification |
| spellingShingle | Şeyma Önlü Züleyha Almaz Aykut Öztekin Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis) Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi cauliflower (brassica oleracea l. var. botrytis) plant tissue culture peroxidase purification |
| title | Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis) |
| title_full | Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis) |
| title_fullStr | Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis) |
| title_full_unstemmed | Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis) |
| title_short | Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis) |
| title_sort | production and purification of peroxidase from tissue cultures of cauliflower brassica oleracea l var botrytis |
| topic | cauliflower (brassica oleracea l. var. botrytis) plant tissue culture peroxidase purification |
| url | https://dergipark.org.tr/tr/download/article-file/1461230 |
| work_keys_str_mv | AT seymaonlu productionandpurificationofperoxidasefromtissueculturesofcauliflowerbrassicaoleracealvarbotrytis AT zuleyhaalmaz productionandpurificationofperoxidasefromtissueculturesofcauliflowerbrassicaoleracealvarbotrytis AT aykutoztekin productionandpurificationofperoxidasefromtissueculturesofcauliflowerbrassicaoleracealvarbotrytis |