Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice
Objective(s): Reducing the immune response to inflammation is vital for successful transplantation, yet chronic graft rejection remains a major issue despite immunosuppressive drugs. This study explored the effect of bone marrow mesenchymal stem cell-derived exosomes on the survival of skin allograf...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2025-04-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_25466_d9fc76c98b3d0c137d91269d1ae77034.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849730563974365184 |
|---|---|
| author | Parham Soufizadeh Gholamreza Nikbakht Brujeni Mohammad Mehdi Dehghan Massoumeh Jabbari Fakhr Pouya Houshmand Mahyar Mohebbi Hossein Aminianfar Sirous Sadeghian Chaleshtori |
| author_facet | Parham Soufizadeh Gholamreza Nikbakht Brujeni Mohammad Mehdi Dehghan Massoumeh Jabbari Fakhr Pouya Houshmand Mahyar Mohebbi Hossein Aminianfar Sirous Sadeghian Chaleshtori |
| author_sort | Parham Soufizadeh |
| collection | DOAJ |
| description | Objective(s): Reducing the immune response to inflammation is vital for successful transplantation, yet chronic graft rejection remains a major issue despite immunosuppressive drugs. This study explored the effect of bone marrow mesenchymal stem cell-derived exosomes on the survival of skin allografts in mice.Materials and Methods: C57BL/6 and BALB/c mice underwent skin allograft surgery, followed by intraperitoneal injection of exosomes, which were compared with groups receiving dexamethasone and no treatment group.Results: On day 3, mild signs of graft rejection appeared in both control groups, while none were seen in the exosome-treated group. By day 14, the grafts were completely rejected in the control groups but showed mild rejection in the treatment group. Histopathology revealed severe rejection signs in the control groups, including epithelial necrosis and inflammation, while the treatment group showed signs of angiogenesis and graft acceptance. Additionally, inflammatory cytokine levels (TNF-α, IL-1β, and IL-6) were lower in the treatment group than in the positive control group, particularly on days 3 and 14.Conclusion: The findings suggest that exosomes can prevent graft rejection and may offer a promising therapeutic approach for solid organ transplantation, though further research is needed to standardize exosome methods and evaluate cost-effectiveness. |
| format | Article |
| id | doaj-art-2c43b664ef9a4155a37235e7dadb80b9 |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-2c43b664ef9a4155a37235e7dadb80b92025-08-20T03:08:50ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-04-0128446947610.22038/ijbms.2025.82564.1784125466Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in miceParham Soufizadeh0Gholamreza Nikbakht Brujeni1Mohammad Mehdi Dehghan2Massoumeh Jabbari Fakhr3Pouya Houshmand4Mahyar Mohebbi5Hossein Aminianfar6Sirous Sadeghian Chaleshtori7Biomedical Research Institute, University of Tehran, Tehran, IranBiomedical Research Institute, University of Tehran, Tehran, IranBiomedical Research Institute, University of Tehran, Tehran, IranBiomedical Research Institute, University of Tehran, Tehran, IranBiomedical Research Institute, University of Tehran, Tehran, IranDepartment of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranBiomedical Research Institute, University of Tehran, Tehran, IranBiomedical Research Institute, University of Tehran, Tehran, IranObjective(s): Reducing the immune response to inflammation is vital for successful transplantation, yet chronic graft rejection remains a major issue despite immunosuppressive drugs. This study explored the effect of bone marrow mesenchymal stem cell-derived exosomes on the survival of skin allografts in mice.Materials and Methods: C57BL/6 and BALB/c mice underwent skin allograft surgery, followed by intraperitoneal injection of exosomes, which were compared with groups receiving dexamethasone and no treatment group.Results: On day 3, mild signs of graft rejection appeared in both control groups, while none were seen in the exosome-treated group. By day 14, the grafts were completely rejected in the control groups but showed mild rejection in the treatment group. Histopathology revealed severe rejection signs in the control groups, including epithelial necrosis and inflammation, while the treatment group showed signs of angiogenesis and graft acceptance. Additionally, inflammatory cytokine levels (TNF-α, IL-1β, and IL-6) were lower in the treatment group than in the positive control group, particularly on days 3 and 14.Conclusion: The findings suggest that exosomes can prevent graft rejection and may offer a promising therapeutic approach for solid organ transplantation, though further research is needed to standardize exosome methods and evaluate cost-effectiveness.https://ijbms.mums.ac.ir/article_25466_d9fc76c98b3d0c137d91269d1ae77034.pdfexosomeimmunomodulationmicemesenchymal stem cellsregenerationtransplantation |
| spellingShingle | Parham Soufizadeh Gholamreza Nikbakht Brujeni Mohammad Mehdi Dehghan Massoumeh Jabbari Fakhr Pouya Houshmand Mahyar Mohebbi Hossein Aminianfar Sirous Sadeghian Chaleshtori Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice Iranian Journal of Basic Medical Sciences exosome immunomodulation mice mesenchymal stem cells regeneration transplantation |
| title | Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice |
| title_full | Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice |
| title_fullStr | Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice |
| title_full_unstemmed | Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice |
| title_short | Preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice |
| title_sort | preventing the rejection of skin allografts by immunomodulatory and regenerative effects of exosomes derived from bone marrow mesenchymal stem cells in mice |
| topic | exosome immunomodulation mice mesenchymal stem cells regeneration transplantation |
| url | https://ijbms.mums.ac.ir/article_25466_d9fc76c98b3d0c137d91269d1ae77034.pdf |
| work_keys_str_mv | AT parhamsoufizadeh preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT gholamrezanikbakhtbrujeni preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT mohammadmehdidehghan preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT massoumehjabbarifakhr preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT pouyahoushmand preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT mahyarmohebbi preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT hosseinaminianfar preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice AT siroussadeghianchaleshtori preventingtherejectionofskinallograftsbyimmunomodulatoryandregenerativeeffectsofexosomesderivedfrombonemarrowmesenchymalstemcellsinmice |