Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)

Entomopathogenic fungi have been widely used for sustainable agriculture as biological pest control agents. However, commercially available isolates were not efficient in controlling the stink bug Euschistus heros (Hemiptera: Pentatomidae), one of the most destructive species in soybean cultivation....

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Main Authors: Aline Nunes-Silva, Camila Costa Moreira, Janaína Brandão Seibert, Jonathan Rodríguez, Italo Delalibera-Júnior
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:Biological Control
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Online Access:http://www.sciencedirect.com/science/article/pii/S1049964425001331
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author Aline Nunes-Silva
Camila Costa Moreira
Janaína Brandão Seibert
Jonathan Rodríguez
Italo Delalibera-Júnior
author_facet Aline Nunes-Silva
Camila Costa Moreira
Janaína Brandão Seibert
Jonathan Rodríguez
Italo Delalibera-Júnior
author_sort Aline Nunes-Silva
collection DOAJ
description Entomopathogenic fungi have been widely used for sustainable agriculture as biological pest control agents. However, commercially available isolates were not efficient in controlling the stink bug Euschistus heros (Hemiptera: Pentatomidae), one of the most destructive species in soybean cultivation. There is an increasing demand for exploring new microorganisms that have the potential to control this insect. The present study evaluated the susceptibility of E. heros to 15 isolates of different species of the genus Metarhizium. A mortality rate of over 95 % was observed in isolates of M. pingshaense, M. humberi, and M. robertsii in 8 days of experiment. The aerial conidia yield of ESALQ 4395 and ESALQ 3364 isolates stood out with a concentration above 1x109 conidia/g of dry rice, which is similar to commercial isolates. The average lethal concentration of these isolates was 5.1x105 conidia/mL for adult insects, with a 95 % confidence interval of 4.7x105 to 5.4x105 conidia/mL for ESALQ 4395 and 4.8x105 to 5.5x105 conidia/mL for ESALQ 3364. However, no age-dependent mortality was observed since mortality among adults was more pronounced than in immature insects. Although low efficiency of entomopathogenic fungi for controlling stink bugs has been associated with the fungistatic and fungicidal action of the volatile compounds present in the alarm pheromone, the data obtained here demonstrated that this insect is highly susceptible to Metarhizium, especially to species that are not explored commercially in Brazil such as M. humberi, M. pinghaense and M. robertsii.
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spelling doaj-art-2c4377b4d5ca4928917b1d68c54744362025-08-20T02:36:08ZengElsevierBiological Control1049-96442025-08-0120710582310.1016/j.biocontrol.2025.105823Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)Aline Nunes-Silva0Camila Costa Moreira1Janaína Brandão Seibert2Jonathan Rodríguez3Italo Delalibera-Júnior4Department of Entomology and Acarology, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo (ESALQ-USP), Av. Pádua Dias, 11, Piracicaba, SP CEP 13418-900, Brazil; Corresponding author.Koppert Biological Systems, Rod. Margarida da Graça Martins, s/n, KM 17,5, Piracicaba, SP, CEP 13400-970, BrazilDepartment of Entomology and Acarology, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo (ESALQ-USP), Av. Pádua Dias, 11, Piracicaba, SP CEP 13418-900, BrazilDepartment of Entomology and Acarology, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo (ESALQ-USP), Av. Pádua Dias, 11, Piracicaba, SP CEP 13418-900, BrazilDepartment of Entomology and Acarology, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo (ESALQ-USP), Av. Pádua Dias, 11, Piracicaba, SP CEP 13418-900, BrazilEntomopathogenic fungi have been widely used for sustainable agriculture as biological pest control agents. However, commercially available isolates were not efficient in controlling the stink bug Euschistus heros (Hemiptera: Pentatomidae), one of the most destructive species in soybean cultivation. There is an increasing demand for exploring new microorganisms that have the potential to control this insect. The present study evaluated the susceptibility of E. heros to 15 isolates of different species of the genus Metarhizium. A mortality rate of over 95 % was observed in isolates of M. pingshaense, M. humberi, and M. robertsii in 8 days of experiment. The aerial conidia yield of ESALQ 4395 and ESALQ 3364 isolates stood out with a concentration above 1x109 conidia/g of dry rice, which is similar to commercial isolates. The average lethal concentration of these isolates was 5.1x105 conidia/mL for adult insects, with a 95 % confidence interval of 4.7x105 to 5.4x105 conidia/mL for ESALQ 4395 and 4.8x105 to 5.5x105 conidia/mL for ESALQ 3364. However, no age-dependent mortality was observed since mortality among adults was more pronounced than in immature insects. Although low efficiency of entomopathogenic fungi for controlling stink bugs has been associated with the fungistatic and fungicidal action of the volatile compounds present in the alarm pheromone, the data obtained here demonstrated that this insect is highly susceptible to Metarhizium, especially to species that are not explored commercially in Brazil such as M. humberi, M. pinghaense and M. robertsii.http://www.sciencedirect.com/science/article/pii/S1049964425001331Biological controlStink bugMetarhizium pingshaenseBiodefensivesSolid fermentation
spellingShingle Aline Nunes-Silva
Camila Costa Moreira
Janaína Brandão Seibert
Jonathan Rodríguez
Italo Delalibera-Júnior
Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)
Biological Control
Biological control
Stink bug
Metarhizium pingshaense
Biodefensives
Solid fermentation
title Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)
title_full Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)
title_fullStr Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)
title_full_unstemmed Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)
title_short Exploring the potential of Metarhizium species for the control of Euschistus heros (Hemiptera: Pentatomidae)
title_sort exploring the potential of metarhizium species for the control of euschistus heros hemiptera pentatomidae
topic Biological control
Stink bug
Metarhizium pingshaense
Biodefensives
Solid fermentation
url http://www.sciencedirect.com/science/article/pii/S1049964425001331
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