Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil
This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine () and rat () fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/648406 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849387219031162880 |
|---|---|
| author | Paloma de Oliveira Tonietti Aline Santana da Hora Fernanda Dornellas F. Silva Karen Linares Ferrari Paulo Eduardo Brandão Leonardo José Richtzenhain Fabio Gregori |
| author_facet | Paloma de Oliveira Tonietti Aline Santana da Hora Fernanda Dornellas F. Silva Karen Linares Ferrari Paulo Eduardo Brandão Leonardo José Richtzenhain Fabio Gregori |
| author_sort | Paloma de Oliveira Tonietti |
| collection | DOAJ |
| description | This study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine () and rat () fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results point toward a cross-species transmission between rats and pigs on the surveyed farm and represent the first detection of rotavirus in Rattus norvegicus in Brazil. |
| format | Article |
| id | doaj-art-2c3eba8236a54ec680015006f605f3b2 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2c3eba8236a54ec680015006f605f3b22025-08-20T03:55:17ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/648406648406Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in BrazilPaloma de Oliveira Tonietti0Aline Santana da Hora1Fernanda Dornellas F. Silva2Karen Linares Ferrari3Paulo Eduardo Brandão4Leonardo José Richtzenhain5Fabio Gregori6Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilDepartment of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, 05508-270 São Paulo, SP, BrazilThis study investigated the occurrence of rotavirus in porcine and Rattus norvegicus, at the same time, on a pig farm in the city of Jaguariúna, São Paulo, Brazil. Swine () and rat () fecal samples were analyzed by nested RT-PCR assay. Rotavirus occurred in seven porcine and two rat samples. A total of three pig and one rat samples were further submitted to genetic sequencing. The partial NSP5 gene phylogeny showed that all strains were segregated in the genotype H1. These results point toward a cross-species transmission between rats and pigs on the surveyed farm and represent the first detection of rotavirus in Rattus norvegicus in Brazil.http://dx.doi.org/10.1155/2013/648406 |
| spellingShingle | Paloma de Oliveira Tonietti Aline Santana da Hora Fernanda Dornellas F. Silva Karen Linares Ferrari Paulo Eduardo Brandão Leonardo José Richtzenhain Fabio Gregori Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil The Scientific World Journal |
| title | Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil |
| title_full | Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil |
| title_fullStr | Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil |
| title_full_unstemmed | Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil |
| title_short | Simultaneous Detection of Group A Rotavirus in Swine and Rat on a Pig Farm in Brazil |
| title_sort | simultaneous detection of group a rotavirus in swine and rat on a pig farm in brazil |
| url | http://dx.doi.org/10.1155/2013/648406 |
| work_keys_str_mv | AT palomadeoliveiratonietti simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil AT alinesantanadahora simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil AT fernandadornellasfsilva simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil AT karenlinaresferrari simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil AT pauloeduardobrandao simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil AT leonardojoserichtzenhain simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil AT fabiogregori simultaneousdetectionofgrouparotavirusinswineandratonapigfarminbrazil |