ICEmST contributes to colonization of Salmonella in the intestine of piglets
Abstract Salmonella enterica serovar 4,[5],12:i:- sequence type 34 (ST34) has recently become a global concern for public and animal health. The acquisition of mobile genetic element ICEmST, which contains two copper tolerance gene clusters, cus and pco, influences the epidemic success of this clone...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-83039-6 |
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| author | Nobuo Arai Tomoyuki Shibahara Reina Nishiura Yukino Tamamura-Andoh Hayate Nishiura Yoshihiro Muneta Hiroshi Sawada Ayako Watanabe-Yanai Taketoshi Iwata Masato Akiba Masahiro Kusumoto |
| author_facet | Nobuo Arai Tomoyuki Shibahara Reina Nishiura Yukino Tamamura-Andoh Hayate Nishiura Yoshihiro Muneta Hiroshi Sawada Ayako Watanabe-Yanai Taketoshi Iwata Masato Akiba Masahiro Kusumoto |
| author_sort | Nobuo Arai |
| collection | DOAJ |
| description | Abstract Salmonella enterica serovar 4,[5],12:i:- sequence type 34 (ST34) has recently become a global concern for public and animal health. The acquisition of mobile genetic element ICEmST, which contains two copper tolerance gene clusters, cus and pco, influences the epidemic success of this clone. Copper is used as a feed additive in swine at levels that potentially lead to selection pressure for Enterobacteriaceae; however, it remains unclear whether the copper tolerance system of ICEmST functions in vivo. We performed competition assays with Salmonella 4,[5],12:i:- ST34 wildtype (WT) and deletion mutants of ICEmST (ΔICEmST, Δcus, and Δpco) in groups of mice fed 0, 150, and 500 ppm CuSO4. In the competition of WT against ΔICEmST and Δcus, the competitive index of the 500 ppm-fed group was significantly lower than that of the 0 ppm-fed group. In the swine experiment, all individuals were fed 150 ppm CuSO4. The number of ICEmST-positive strain in the feces was significantly greater than that of ICEmST-negative strain. The serum inflammatory markers were significantly increased in swine infected with the ICEmST-positive strain. These data suggest that ICEmST, especially cus, provides Salmonella with the ability to colonize in the intestine, even at high copper concentrations, leading to swine salmonellosis. |
| format | Article |
| id | doaj-art-2c3beb233fe34b83a3344b695a4db418 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-2c3beb233fe34b83a3344b695a4db4182025-08-20T02:39:38ZengNature PortfolioScientific Reports2045-23222024-12-0114111010.1038/s41598-024-83039-6ICEmST contributes to colonization of Salmonella in the intestine of pigletsNobuo Arai0Tomoyuki Shibahara1Reina Nishiura2Yukino Tamamura-Andoh3Hayate Nishiura4Yoshihiro Muneta5Hiroshi Sawada6Ayako Watanabe-Yanai7Taketoshi Iwata8Masato Akiba9Masahiro Kusumoto10Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Hygiene Management Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Hygiene Management Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Hygiene Management Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Hygiene Management Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationDivision of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationLaboratory of Veterinary Bacteriology, School of Veterinary Medicine, Rakuno Gakuen UniversityDivision of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research OrganizationAbstract Salmonella enterica serovar 4,[5],12:i:- sequence type 34 (ST34) has recently become a global concern for public and animal health. The acquisition of mobile genetic element ICEmST, which contains two copper tolerance gene clusters, cus and pco, influences the epidemic success of this clone. Copper is used as a feed additive in swine at levels that potentially lead to selection pressure for Enterobacteriaceae; however, it remains unclear whether the copper tolerance system of ICEmST functions in vivo. We performed competition assays with Salmonella 4,[5],12:i:- ST34 wildtype (WT) and deletion mutants of ICEmST (ΔICEmST, Δcus, and Δpco) in groups of mice fed 0, 150, and 500 ppm CuSO4. In the competition of WT against ΔICEmST and Δcus, the competitive index of the 500 ppm-fed group was significantly lower than that of the 0 ppm-fed group. In the swine experiment, all individuals were fed 150 ppm CuSO4. The number of ICEmST-positive strain in the feces was significantly greater than that of ICEmST-negative strain. The serum inflammatory markers were significantly increased in swine infected with the ICEmST-positive strain. These data suggest that ICEmST, especially cus, provides Salmonella with the ability to colonize in the intestine, even at high copper concentrations, leading to swine salmonellosis.https://doi.org/10.1038/s41598-024-83039-6Copper toleranceFeedICEmSTSalmonella 4,[5],12:i:-Swine |
| spellingShingle | Nobuo Arai Tomoyuki Shibahara Reina Nishiura Yukino Tamamura-Andoh Hayate Nishiura Yoshihiro Muneta Hiroshi Sawada Ayako Watanabe-Yanai Taketoshi Iwata Masato Akiba Masahiro Kusumoto ICEmST contributes to colonization of Salmonella in the intestine of piglets Scientific Reports Copper tolerance Feed ICEmST Salmonella 4,[5],12:i:- Swine |
| title | ICEmST contributes to colonization of Salmonella in the intestine of piglets |
| title_full | ICEmST contributes to colonization of Salmonella in the intestine of piglets |
| title_fullStr | ICEmST contributes to colonization of Salmonella in the intestine of piglets |
| title_full_unstemmed | ICEmST contributes to colonization of Salmonella in the intestine of piglets |
| title_short | ICEmST contributes to colonization of Salmonella in the intestine of piglets |
| title_sort | icemst contributes to colonization of salmonella in the intestine of piglets |
| topic | Copper tolerance Feed ICEmST Salmonella 4,[5],12:i:- Swine |
| url | https://doi.org/10.1038/s41598-024-83039-6 |
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