The Prospect of Gene Exploitation through Soil Metagenomics

The quantity and diversity of the microbial community in soil make it possibly the most difficult of all the natural ecosystems. It is thought to be challenging to culture up to 99% of the microorganisms in a given environment. The intricacy of microbial variety is impacted by numerous interconnecte...

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Main Authors: Kalpana Sagar, Km. Priti, Kartikey Kumar Gupta, Annapurna Katara, Archana Yadav, Harish Chandra
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2024-12-01
Series:Journal of Pure and Applied Microbiology
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Online Access:https://microbiologyjournal.org/the-prospect-of-gene-exploitation-through-soil-metagenomics/
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author Kalpana Sagar
Km. Priti
Kartikey Kumar Gupta
Annapurna Katara
Archana Yadav
Harish Chandra
author_facet Kalpana Sagar
Km. Priti
Kartikey Kumar Gupta
Annapurna Katara
Archana Yadav
Harish Chandra
author_sort Kalpana Sagar
collection DOAJ
description The quantity and diversity of the microbial community in soil make it possibly the most difficult of all the natural ecosystems. It is thought to be challenging to culture up to 99% of the microorganisms in a given environment. The intricacy of microbial variety is impacted by numerous interconnected factors, including as soil structure, water content, biotic activity, pH, and fluctuations in climate. Environmental DNA isolation and purification are often the first steps in the soil metagenomic analysis process. Creating genomic DNA libraries and then using them for high-throughput sequencing or library screening are the main steps in the application of metagenomics. These genomic sequences are currently being used to advance our knowledge of the ecology and physiology of these bacteria as well as for new biotechnological and medicinal applications. To completely comprehend the intricacies involved in the operation of microbial communities and the interactions between different microorganisms within specific niches, metagenomic sequences are employed. This study focuses on the latest advancements in biotechnological approaches and procedures for identifying novel genes from uncultured microorganisms and intricate microbial habitats.
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institution OA Journals
issn 0973-7510
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language English
publishDate 2024-12-01
publisher Journal of Pure and Applied Microbiology
record_format Article
series Journal of Pure and Applied Microbiology
spelling doaj-art-2c2fac56dff3497498afad3bc89ad40f2025-08-20T01:55:08ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2024-12-0118422282243https://doi.org/10.22207/JPAM.18.4.60The Prospect of Gene Exploitation through Soil MetagenomicsKalpana Sagarhttps://orcid.org/0000-0001-8140-4434Km. Pritihttps://orcid.org/0009-0009-7640-2691Kartikey Kumar Guptahttps://orcid.org/0000-0001-6264-6382Annapurna Katarahttps://orcid.org/0009-0007-5999-1274Archana Yadavhttps://orcid.org/0009-0009-4434-9881Harish Chandrahttps://orcid.org/0000-0002-0448-0174The quantity and diversity of the microbial community in soil make it possibly the most difficult of all the natural ecosystems. It is thought to be challenging to culture up to 99% of the microorganisms in a given environment. The intricacy of microbial variety is impacted by numerous interconnected factors, including as soil structure, water content, biotic activity, pH, and fluctuations in climate. Environmental DNA isolation and purification are often the first steps in the soil metagenomic analysis process. Creating genomic DNA libraries and then using them for high-throughput sequencing or library screening are the main steps in the application of metagenomics. These genomic sequences are currently being used to advance our knowledge of the ecology and physiology of these bacteria as well as for new biotechnological and medicinal applications. To completely comprehend the intricacies involved in the operation of microbial communities and the interactions between different microorganisms within specific niches, metagenomic sequences are employed. This study focuses on the latest advancements in biotechnological approaches and procedures for identifying novel genes from uncultured microorganisms and intricate microbial habitats.https://microbiologyjournal.org/the-prospect-of-gene-exploitation-through-soil-metagenomics/metagenomicshumic compoundsnovel compoundsmetagenomic librariesphylogenetic markers
spellingShingle Kalpana Sagar
Km. Priti
Kartikey Kumar Gupta
Annapurna Katara
Archana Yadav
Harish Chandra
The Prospect of Gene Exploitation through Soil Metagenomics
Journal of Pure and Applied Microbiology
metagenomics
humic compounds
novel compounds
metagenomic libraries
phylogenetic markers
title The Prospect of Gene Exploitation through Soil Metagenomics
title_full The Prospect of Gene Exploitation through Soil Metagenomics
title_fullStr The Prospect of Gene Exploitation through Soil Metagenomics
title_full_unstemmed The Prospect of Gene Exploitation through Soil Metagenomics
title_short The Prospect of Gene Exploitation through Soil Metagenomics
title_sort prospect of gene exploitation through soil metagenomics
topic metagenomics
humic compounds
novel compounds
metagenomic libraries
phylogenetic markers
url https://microbiologyjournal.org/the-prospect-of-gene-exploitation-through-soil-metagenomics/
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