Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season
Background: In older patients, it is important to rapidly identify those infected by a significant pathogen in order to implement isolation measures and prescribe appropriate specific treatments when available and indicated. The objective of this study was to evaluate a multiplex genomic point-of-ca...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
|
| Series: | Journal of Infection and Public Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034125001443 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850188169205514240 |
|---|---|
| author | Marion Fayolle Ludovic Lafaie Thomas Franck Marie Blanquet Fabien de Oliveira Thomas Bourlet Bruno Pozzetto Sylvie Pillet Thomas Célarier |
| author_facet | Marion Fayolle Ludovic Lafaie Thomas Franck Marie Blanquet Fabien de Oliveira Thomas Bourlet Bruno Pozzetto Sylvie Pillet Thomas Célarier |
| author_sort | Marion Fayolle |
| collection | DOAJ |
| description | Background: In older patients, it is important to rapidly identify those infected by a significant pathogen in order to implement isolation measures and prescribe appropriate specific treatments when available and indicated. The objective of this study was to evaluate a multiplex genomic point-of-care (POC) strategy detecting four viruses, SARS-CoV-2, influenza virus (IV) A and B and respiratory syncytial virus (RSV), in a geriatric setting located 7 km from the central laboratory of the hospital. Methods: This prospective monocentric study was conducted during the 2022–2023 winter season in two geriatric wards of the University Hospital of Saint-Etienne, France. Two automated quantitative reverse transcription polymerase chain reaction (RT-qPCR) tests were compared, the reference test at the central laboratory and the POC test performed by nurses. The main outcome was the saved time to result (TTR) by the clinician with the POC technology. Additional objectives included testing performance, health-economic considerations and healthcare workers’ acceptability. Results: From 69 included infectious episodes, the reference test identified 18 viral infections (7 for SARS-CoV-2, 8 for RSV, 2 for both viruses, 1 for IVA). The POC test yielded similar performance with reference test (overall concordance of 98.55 % for SARS-CoV-2 and RSV). The POC strategy showed a gain of 10.43 ( ± 6.92) hours on TTR and a cumulative time of isolation of 488.27 hours in case of negative result. Further results showed economic savings and a strong acceptability by healthcare workers in favor of the POC strategy. Conclusions: This pilot study illustrates the benefits of a genomic POC test to identify current respiratory viral infections in older people within a geriatric setting remote from the central laboratory during a winter season with multiple viral outbreaks. |
| format | Article |
| id | doaj-art-2c27971ae62a482e9ddebfb27a0ad009 |
| institution | OA Journals |
| issn | 1876-0341 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj-art-2c27971ae62a482e9ddebfb27a0ad0092025-08-20T02:15:58ZengElsevierJournal of Infection and Public Health1876-03412025-07-0118710279510.1016/j.jiph.2025.102795Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter seasonMarion Fayolle0Ludovic Lafaie1Thomas Franck2Marie Blanquet3Fabien de Oliveira4Thomas Bourlet5Bruno Pozzetto6Sylvie Pillet7Thomas Célarier8Department of Gerontology, Hôpital de la Charité, University Hospital of Saint-Etienne, Saint-Etienne, FranceDepartment of Gerontology, Hôpital de la Charité, University Hospital of Saint-Etienne, Saint-Etienne, France; Gérontopole Auvergne Rhône-Alpes, Hôpital Bellevue, Saint-Etienne, FranceGérontopole Auvergne Rhône-Alpes, Hôpital Bellevue, Saint-Etienne, FrancePublic Health Department, Hôpital Gabriel Montpied, University Hospital of Clermont-Ferrand, Clermont-Ferrand, FranceDepartment of Medical Imaging, IMAGINE UR UM 103, Montpellier University, Nîmes University Hospital, Nîmes 30900, FranceDepartment of Microbiology, Hôpital Nord, University Hospital of Saint-Etienne, France; Centre International de Recherche en Infectiologie (CIRI), GIMAP team, Inserm U1111, CNRS UMR, Lyon-Saint-Etienne 5308, FranceDepartment of Microbiology, Hôpital Nord, University Hospital of Saint-Etienne, France; Centre International de Recherche en Infectiologie (CIRI), GIMAP team, Inserm U1111, CNRS UMR, Lyon-Saint-Etienne 5308, France; Correspondence to: Department of Infectious Agents and Hygiene, Avenue Albert Raimond, Hôpital Nord, CHU de Saint-Etienne, Saint-Etienne 42055 cedex 2, France.Department of Microbiology, Hôpital Nord, University Hospital of Saint-Etienne, France; Centre International de Recherche en Infectiologie (CIRI), GIMAP team, Inserm U1111, CNRS UMR, Lyon-Saint-Etienne 5308, FranceDepartment of Gerontology, Hôpital de la Charité, University Hospital of Saint-Etienne, Saint-Etienne, France; Gérontopole Auvergne Rhône-Alpes, Hôpital Bellevue, Saint-Etienne, FranceBackground: In older patients, it is important to rapidly identify those infected by a significant pathogen in order to implement isolation measures and prescribe appropriate specific treatments when available and indicated. The objective of this study was to evaluate a multiplex genomic point-of-care (POC) strategy detecting four viruses, SARS-CoV-2, influenza virus (IV) A and B and respiratory syncytial virus (RSV), in a geriatric setting located 7 km from the central laboratory of the hospital. Methods: This prospective monocentric study was conducted during the 2022–2023 winter season in two geriatric wards of the University Hospital of Saint-Etienne, France. Two automated quantitative reverse transcription polymerase chain reaction (RT-qPCR) tests were compared, the reference test at the central laboratory and the POC test performed by nurses. The main outcome was the saved time to result (TTR) by the clinician with the POC technology. Additional objectives included testing performance, health-economic considerations and healthcare workers’ acceptability. Results: From 69 included infectious episodes, the reference test identified 18 viral infections (7 for SARS-CoV-2, 8 for RSV, 2 for both viruses, 1 for IVA). The POC test yielded similar performance with reference test (overall concordance of 98.55 % for SARS-CoV-2 and RSV). The POC strategy showed a gain of 10.43 ( ± 6.92) hours on TTR and a cumulative time of isolation of 488.27 hours in case of negative result. Further results showed economic savings and a strong acceptability by healthcare workers in favor of the POC strategy. Conclusions: This pilot study illustrates the benefits of a genomic POC test to identify current respiratory viral infections in older people within a geriatric setting remote from the central laboratory during a winter season with multiple viral outbreaks.http://www.sciencedirect.com/science/article/pii/S1876034125001443Point-of-care diagnosisgenomic multiplex testingseasonal viral respiratory infectionolder peoplesingle-room isolationviral outbreak |
| spellingShingle | Marion Fayolle Ludovic Lafaie Thomas Franck Marie Blanquet Fabien de Oliveira Thomas Bourlet Bruno Pozzetto Sylvie Pillet Thomas Célarier Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season Journal of Infection and Public Health Point-of-care diagnosis genomic multiplex testing seasonal viral respiratory infection older people single-room isolation viral outbreak |
| title | Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season |
| title_full | Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season |
| title_fullStr | Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season |
| title_full_unstemmed | Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season |
| title_short | Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season |
| title_sort | evaluation of a multiplex genomic point of care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season |
| topic | Point-of-care diagnosis genomic multiplex testing seasonal viral respiratory infection older people single-room isolation viral outbreak |
| url | http://www.sciencedirect.com/science/article/pii/S1876034125001443 |
| work_keys_str_mv | AT marionfayolle evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT ludoviclafaie evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT thomasfranck evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT marieblanquet evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT fabiendeoliveira evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT thomasbourlet evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT brunopozzetto evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT sylviepillet evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason AT thomascelarier evaluationofamultiplexgenomicpointofcarestrategyforrapidsupportofolderpeoplewithrespiratoryinfectioninageriatrichospitaloverafullwinterseason |