Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study
Several areas of the world suffer a notably high incidence of Shiga toxin-producing Escherichia coli. To assess the impact of persistent cross-species transmission systems on the epidemiology of E. coli O157:H7 in Alberta, Canada, we sequenced and assembled E. coli O157:H7 isolates originating from...
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eLife Sciences Publications Ltd
2025-01-01
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| Online Access: | https://elifesciences.org/articles/97643 |
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| author | Gillian AM Tarr Linda Chui Kim Stanford Emmanuel W Bumunang Rahat Zaheer Vincent Li Stephen B Freedman Chad R Laing Tim A McAllister |
| author_facet | Gillian AM Tarr Linda Chui Kim Stanford Emmanuel W Bumunang Rahat Zaheer Vincent Li Stephen B Freedman Chad R Laing Tim A McAllister |
| author_sort | Gillian AM Tarr |
| collection | DOAJ |
| description | Several areas of the world suffer a notably high incidence of Shiga toxin-producing Escherichia coli. To assess the impact of persistent cross-species transmission systems on the epidemiology of E. coli O157:H7 in Alberta, Canada, we sequenced and assembled E. coli O157:H7 isolates originating from collocated cattle and human populations, 2007–2015. We constructed a timed phylogeny using BEAST2 using a structured coalescent model. We then extended the tree with human isolates through 2019 to assess the long-term disease impact of locally persistent lineages. During 2007–2015, we estimated that 88.5% of human lineages arose from cattle lineages. We identified 11 persistent lineages local to Alberta, which were associated with 38.0% (95% CI 29.3%, 47.3%) of human isolates. During the later period, six locally persistent lineages continued to be associated with human illness, including 74.7% (95% CI 68.3%, 80.3%) of reported cases in 2018 and 2019. Our study identified multiple locally evolving lineages transmitted between cattle and humans persistently associated with E. coli O157:H7 illnesses for up to 13 y. Locally persistent lineages may be a principal cause of the high incidence of E. coli O157:H7 in locations such as Alberta and provide opportunities for focused control efforts. |
| format | Article |
| id | doaj-art-2c23ae953f5341e786486435c38b70a1 |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj-art-2c23ae953f5341e786486435c38b70a12025-01-29T12:39:50ZengeLife Sciences Publications LtdeLife2050-084X2025-01-011310.7554/eLife.97643Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology studyGillian AM Tarr0https://orcid.org/0000-0001-7372-1034Linda Chui1Kim Stanford2Emmanuel W Bumunang3Rahat Zaheer4Vincent Li5Stephen B Freedman6Chad R Laing7Tim A McAllister8Division of Environmental Health Sciences, School of Public Health, University of Minnesota, Minneapolis, United StatesAlberta Precision Laboratories, Alberta Public Health, Walter Mackenzie Health Sciences Centre, Edmonton, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, CanadaDepartment of Biological Sciences, University of Lethbridge, Lethbridge, CanadaDepartment of Biological Sciences, University of Lethbridge, Lethbridge, CanadaAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, CanadaAlberta Precision Laboratories, Alberta Public Health, Walter Mackenzie Health Sciences Centre, Edmonton, CanadaSections of Pediatric Emergency Medicine and Gastroenterology, Department of Pediatrics, Alberta Children’s Hospital and Alberta Children’s Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, CanadaNational Center for Animal Diseases Lethbridge Laboratory, Canadian Food Inspection Agency, Lethbridge County, CanadaAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, CanadaSeveral areas of the world suffer a notably high incidence of Shiga toxin-producing Escherichia coli. To assess the impact of persistent cross-species transmission systems on the epidemiology of E. coli O157:H7 in Alberta, Canada, we sequenced and assembled E. coli O157:H7 isolates originating from collocated cattle and human populations, 2007–2015. We constructed a timed phylogeny using BEAST2 using a structured coalescent model. We then extended the tree with human isolates through 2019 to assess the long-term disease impact of locally persistent lineages. During 2007–2015, we estimated that 88.5% of human lineages arose from cattle lineages. We identified 11 persistent lineages local to Alberta, which were associated with 38.0% (95% CI 29.3%, 47.3%) of human isolates. During the later period, six locally persistent lineages continued to be associated with human illness, including 74.7% (95% CI 68.3%, 80.3%) of reported cases in 2018 and 2019. Our study identified multiple locally evolving lineages transmitted between cattle and humans persistently associated with E. coli O157:H7 illnesses for up to 13 y. Locally persistent lineages may be a principal cause of the high incidence of E. coli O157:H7 in locations such as Alberta and provide opportunities for focused control efforts.https://elifesciences.org/articles/97643STECgenomic epidemiologyzoonosisE. coli O157 |
| spellingShingle | Gillian AM Tarr Linda Chui Kim Stanford Emmanuel W Bumunang Rahat Zaheer Vincent Li Stephen B Freedman Chad R Laing Tim A McAllister Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study eLife STEC genomic epidemiology zoonosis E. coli O157 |
| title | Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study |
| title_full | Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study |
| title_fullStr | Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study |
| title_full_unstemmed | Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study |
| title_short | Persistent cross-species transmission systems dominate Shiga toxin-producing Escherichia coli O157:H7 epidemiology in a high incidence region: A genomic epidemiology study |
| title_sort | persistent cross species transmission systems dominate shiga toxin producing escherichia coli o157 h7 epidemiology in a high incidence region a genomic epidemiology study |
| topic | STEC genomic epidemiology zoonosis E. coli O157 |
| url | https://elifesciences.org/articles/97643 |
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