The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists
Sphingolipids and glycosphingolipids are among the most structurally diverse and complex compounds in the mammalian metabolome. They are well known to play important roles in biological architecture, cell-cell communication, and cellular regulation, and for many biological processes, multiple sphing...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227525000732 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849419716315054080 |
|---|---|
| author | Yusuf A. Hannun Alfred H. Merrill, Jr. Chiara Luberto |
| author_facet | Yusuf A. Hannun Alfred H. Merrill, Jr. Chiara Luberto |
| author_sort | Yusuf A. Hannun |
| collection | DOAJ |
| description | Sphingolipids and glycosphingolipids are among the most structurally diverse and complex compounds in the mammalian metabolome. They are well known to play important roles in biological architecture, cell-cell communication, and cellular regulation, and for many biological processes, multiple sphingolipids are involved. Thus, it is not surprising that untargeted genetic/transcriptomic/pharmacologic/metabolomic screens have uncovered changes in sphingolipids and sphingolipid genes/proteins while studying physiological and pathological processes. Consequently, with increasing frequency, both targeted and untargeted mass spectrometry methodologies are being used to conduct sphingolipidomic analyses. Interpretation of such large data sets and design of follow-up experiments can be daunting for investigators with limited expertise with sphingolipids (and sometimes even for someone well-versed in sphingolipidology). Therefore, this review gives an overview of essential elements of sphingolipid structure and analysis, metabolism, functions, and roles in disease and discusses some of the items to consider when interpreting lipidomics data and designing follow-up investigations. |
| format | Article |
| id | doaj-art-2c16f99cc4ff4b289b612a4dcd68db28 |
| institution | Kabale University |
| issn | 0022-2275 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj-art-2c16f99cc4ff4b289b612a4dcd68db282025-08-20T03:32:00ZengElsevierJournal of Lipid Research0022-22752025-06-0166610081310.1016/j.jlr.2025.100813The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologistsYusuf A. Hannun0Alfred H. Merrill, Jr.1Chiara Luberto2Departments of Biochemistry, Medicine, and the Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY, USA; For correspondence: Yusuf A. Hannun; Alfred H. Merrill; Chiara LubertoSchool of Biological Sciences and the Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA, USA; For correspondence: Yusuf A. Hannun; Alfred H. Merrill; Chiara LubertoDepartment of Physiology and Biophysics, and the Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY, USA; For correspondence: Yusuf A. Hannun; Alfred H. Merrill; Chiara LubertoSphingolipids and glycosphingolipids are among the most structurally diverse and complex compounds in the mammalian metabolome. They are well known to play important roles in biological architecture, cell-cell communication, and cellular regulation, and for many biological processes, multiple sphingolipids are involved. Thus, it is not surprising that untargeted genetic/transcriptomic/pharmacologic/metabolomic screens have uncovered changes in sphingolipids and sphingolipid genes/proteins while studying physiological and pathological processes. Consequently, with increasing frequency, both targeted and untargeted mass spectrometry methodologies are being used to conduct sphingolipidomic analyses. Interpretation of such large data sets and design of follow-up experiments can be daunting for investigators with limited expertise with sphingolipids (and sometimes even for someone well-versed in sphingolipidology). Therefore, this review gives an overview of essential elements of sphingolipid structure and analysis, metabolism, functions, and roles in disease and discusses some of the items to consider when interpreting lipidomics data and designing follow-up investigations.http://www.sciencedirect.com/science/article/pii/S0022227525000732pathway analysistargeted lipidomicsuntargeted lipidomics |
| spellingShingle | Yusuf A. Hannun Alfred H. Merrill, Jr. Chiara Luberto The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists Journal of Lipid Research pathway analysis targeted lipidomics untargeted lipidomics |
| title | The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists |
| title_full | The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists |
| title_fullStr | The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists |
| title_full_unstemmed | The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists |
| title_short | The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists |
| title_sort | bioactive sphingolipid playbook a primer for the uninitiated as well as sphingolipidologists |
| topic | pathway analysis targeted lipidomics untargeted lipidomics |
| url | http://www.sciencedirect.com/science/article/pii/S0022227525000732 |
| work_keys_str_mv | AT yusufahannun thebioactivesphingolipidplaybookaprimerfortheuninitiatedaswellassphingolipidologists AT alfredhmerrilljr thebioactivesphingolipidplaybookaprimerfortheuninitiatedaswellassphingolipidologists AT chiaraluberto thebioactivesphingolipidplaybookaprimerfortheuninitiatedaswellassphingolipidologists AT yusufahannun bioactivesphingolipidplaybookaprimerfortheuninitiatedaswellassphingolipidologists AT alfredhmerrilljr bioactivesphingolipidplaybookaprimerfortheuninitiatedaswellassphingolipidologists AT chiaraluberto bioactivesphingolipidplaybookaprimerfortheuninitiatedaswellassphingolipidologists |