ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE.
Johne's disease is one of the main causes of economic losses in ruminants and a major health hazard in the developed and developing developed countries.In this study, PCR detection of insertion sequences IS900 of MAP in the buffy coat of cows (n = 81), of this 29 (35.8%) cow s...
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College of Veterinary Medicine, University of Basrah, Iraq
2019-06-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_162857_211bcd6563cb7b06e90b88f27cab1aec.pdf |
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| description | Johne's disease is one of the main causes of economic losses in ruminants and a major health hazard in the developed and developing developed countries.In this study, PCR detection of insertion sequences IS900 of MAP in the buffy coat of cows (n = 81), of this 29 (35.8%) cow showed positive results. By Restriction fragment length polymorphism (PCR-RFLP),two single nucleotide polymorphisms (SNPs) of SLC11A1 gene were tested for finding their association with susceptibility to bovine Johens disease in Iraqi cattle. A total of 50 cows were tested, theirresult revealedthat at rs109453173 locus twoelectromorph‘CC’ (374 bp) and ‘CG’ (374, 293 and 81 bp).The rs109915208 locus also showed two electromorph, ‘TT (344bp) and ‘CT’ (344, 215 and 129 bp) . The differences in the electromorph between IS900 positive and negative cows were found to be statistically significant (p = 0.0031). No significant difference in these electromorph at SNP locus rs109915208 between IS900 positive and negative cows. Out of two SNPs from SLC11A1 gene, rs109453173 had a significant association with the susceptibility to Johne's disease. The CC’ electromorph observed at rs109453173 locus showed a significant association with the susceptibility to bovine paratuberculosis in cows. The OR of ‘CC’ in‘IS900 positive versusIS900 negative cattle was7.8750, suggesting that cows having ‘CC’ electromorph were susceptible to Johne's disease compared to ‘CG’ electromorph . |
| format | Article |
| id | doaj-art-2c11133b627a428dbcc3ee072ca130f5 |
| institution | OA Journals |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2019-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-2c11133b627a428dbcc3ee072ca130f52025-08-20T01:50:53ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562019-06-0118126828210.33762/bvetr.2019.162857162857ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE.Johne's disease is one of the main causes of economic losses in ruminants and a major health hazard in the developed and developing developed countries.In this study, PCR detection of insertion sequences IS900 of MAP in the buffy coat of cows (n = 81), of this 29 (35.8%) cow showed positive results. By Restriction fragment length polymorphism (PCR-RFLP),two single nucleotide polymorphisms (SNPs) of SLC11A1 gene were tested for finding their association with susceptibility to bovine Johens disease in Iraqi cattle. A total of 50 cows were tested, theirresult revealedthat at rs109453173 locus twoelectromorph‘CC’ (374 bp) and ‘CG’ (374, 293 and 81 bp).The rs109915208 locus also showed two electromorph, ‘TT (344bp) and ‘CT’ (344, 215 and 129 bp) . The differences in the electromorph between IS900 positive and negative cows were found to be statistically significant (p = 0.0031). No significant difference in these electromorph at SNP locus rs109915208 between IS900 positive and negative cows. Out of two SNPs from SLC11A1 gene, rs109453173 had a significant association with the susceptibility to Johne's disease. The CC’ electromorph observed at rs109453173 locus showed a significant association with the susceptibility to bovine paratuberculosis in cows. The OR of ‘CC’ in‘IS900 positive versusIS900 negative cattle was7.8750, suggesting that cows having ‘CC’ electromorph were susceptible to Johne's disease compared to ‘CG’ electromorph . https://bjvr.uobasrah.edu.iq/article_162857_211bcd6563cb7b06e90b88f27cab1aec.pdfcattlejohnes disease(pcr-rflp) |
| spellingShingle | ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. Basrah Journal of Veterinary Research cattle johnes disease (pcr-rflp) |
| title | ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. |
| title_full | ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. |
| title_fullStr | ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. |
| title_full_unstemmed | ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. |
| title_short | ASSOCIATION OF POLYMORPHISMS IN SLC11A1 GENE WITH AUTOIMMUNITY CAUSED BY Mycobacterium avium subspecies paratuberculosis(MAP) IN CATTLE. |
| title_sort | association of polymorphisms in slc11a1 gene with autoimmunity caused by mycobacterium avium subspecies paratuberculosis map in cattle |
| topic | cattle johnes disease (pcr-rflp) |
| url | https://bjvr.uobasrah.edu.iq/article_162857_211bcd6563cb7b06e90b88f27cab1aec.pdf |