Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet
[Objective] NAC transcription factors play a significant role in plant resistance to abiotic stress. In this study, the NAC transcription factor gene BvNAC40 was cloned from the drought-resistant sugar beet variety ‘HI0466’, and its protein structure and expression under drought stress were analyz...
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| Format: | Article |
| Language: | zho |
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Science Press
2025-01-01
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| Series: | Xibei zhiwu xuebao |
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| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240445?st=article_issue |
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| _version_ | 1823858105244450816 |
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| author | FAN Ziqi SUN Yaqing LI Ningning ZHANG Shaoying LI Guolong |
| author_facet | FAN Ziqi SUN Yaqing LI Ningning ZHANG Shaoying LI Guolong |
| author_sort | FAN Ziqi |
| collection | DOAJ |
| description | [Objective] NAC transcription factors play a significant role in plant resistance to abiotic stress.
In this study, the NAC transcription factor gene BvNAC40 was cloned from the drought-resistant sugar
beet variety ‘HI0466’, and its protein structure and expression under drought stress were analyzed. This
research provided a theoretical basis for further analysis of BvNAC40 and the breeding of drought-resistant
sugar beet varieties. [Methods] BvNAC40 gene was cloned using the PCR method, and its protein structure
was analyzed by bioinformatics methods. Subcellular localization was observed with green fluorescent
protein (GFP). Expression of BvNAC40 under drought stress was analyzed by qRT-PCR. Overexpression
vectors and virus-induced gene silencing (VIGS) vectors for BvNAC40 were constructed using homologous
recombination. [Results] The CDS of BvNAC40 was 1 182 bp long, encoding 393 amino acids, which was
classified within the NAC transcription factor family, with a transmembrane domain at the C-terminus.
Subcellular localization showed that it was primarily located in the nucleus. Under drought stress, BvNAC40 expression
was upregulated in both the leaves and roots of sugar beets. [Conclusion] The BvNAC40 gene is specifically
expressed in response to drought stress in sugar beets, laying the foundation for future studies using transgenic
technology. |
| format | Article |
| id | doaj-art-2c10ea3ddda54ee0aed3a68c744e9f9b |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-2c10ea3ddda54ee0aed3a68c744e9f9b2025-02-11T14:01:22ZzhoScience PressXibei zhiwu xuebao1000-40252025-01-014510028003610.7606/j.issn.1000-4025.20240445Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beetFAN Ziqi0SUN Yaqing1LI Ningning2ZHANG Shaoying3LI Guolong4College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, China[Objective] NAC transcription factors play a significant role in plant resistance to abiotic stress. In this study, the NAC transcription factor gene BvNAC40 was cloned from the drought-resistant sugar beet variety ‘HI0466’, and its protein structure and expression under drought stress were analyzed. This research provided a theoretical basis for further analysis of BvNAC40 and the breeding of drought-resistant sugar beet varieties. [Methods] BvNAC40 gene was cloned using the PCR method, and its protein structure was analyzed by bioinformatics methods. Subcellular localization was observed with green fluorescent protein (GFP). Expression of BvNAC40 under drought stress was analyzed by qRT-PCR. Overexpression vectors and virus-induced gene silencing (VIGS) vectors for BvNAC40 were constructed using homologous recombination. [Results] The CDS of BvNAC40 was 1 182 bp long, encoding 393 amino acids, which was classified within the NAC transcription factor family, with a transmembrane domain at the C-terminus. Subcellular localization showed that it was primarily located in the nucleus. Under drought stress, BvNAC40 expression was upregulated in both the leaves and roots of sugar beets. [Conclusion] The BvNAC40 gene is specifically expressed in response to drought stress in sugar beets, laying the foundation for future studies using transgenic technology.http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240445?st=article_issuesugar beetnac transcription factordrought stressexpression patternvector construction |
| spellingShingle | FAN Ziqi SUN Yaqing LI Ningning ZHANG Shaoying LI Guolong Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet Xibei zhiwu xuebao sugar beet nac transcription factor drought stress expression pattern vector construction |
| title | Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet |
| title_full | Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet |
| title_fullStr | Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet |
| title_full_unstemmed | Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet |
| title_short | Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet |
| title_sort | cloning expression pattern analysis and vector construction of the bvnac40 gene in sugar beet |
| topic | sugar beet nac transcription factor drought stress expression pattern vector construction |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240445?st=article_issue |
| work_keys_str_mv | AT fanziqi cloningexpressionpatternanalysisandvectorconstructionofthebvnac40geneinsugarbeet AT sunyaqing cloningexpressionpatternanalysisandvectorconstructionofthebvnac40geneinsugarbeet AT liningning cloningexpressionpatternanalysisandvectorconstructionofthebvnac40geneinsugarbeet AT zhangshaoying cloningexpressionpatternanalysisandvectorconstructionofthebvnac40geneinsugarbeet AT liguolong cloningexpressionpatternanalysisandvectorconstructionofthebvnac40geneinsugarbeet |