Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.)
With climate change, plants face numerous stresses, notably drought for rice cultivation. Improving rice drought tolerance is vital for sustainable production in water-scarce regions. Identification of drought tolerant genotypes at the seedling stage of the crop contributes to build a climate resili...
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Czech Academy of Agricultural Sciences
2025-01-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-202501-0001_unravelling-population-structure-and-marker-trait-association-using-ssr-markers-among-the-identified-drought-to.php |
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| author | Shanmugam Manju Devi John Amalraj Joel Muthurajan Raveendran Ramamoorthy Pushpam Sengalan Muthuramu Raman Pushpa N. Sritharan Periyasamy Prasanna Ramalingam Suresh |
| author_facet | Shanmugam Manju Devi John Amalraj Joel Muthurajan Raveendran Ramamoorthy Pushpam Sengalan Muthuramu Raman Pushpa N. Sritharan Periyasamy Prasanna Ramalingam Suresh |
| author_sort | Shanmugam Manju Devi |
| collection | DOAJ |
| description | With climate change, plants face numerous stresses, notably drought for rice cultivation. Improving rice drought tolerance is vital for sustainable production in water-scarce regions. Identification of drought tolerant genotypes at the seedling stage of the crop contributes to build a climate resilient genotype during the period of water scarcity and under challenging environmental conditions. Hence, polyethylene glycol-6000 (PEG-6000) induced drought conditions could be used for testing the drought tolerance in rice at an earlier stage of the crop. Optimization of PEG-6000 concentration for screening index at -6 bar was done using three drought-tolerant and two drought-susceptible check varieties based on probit analysis. Subsequently, 100 rice landraces underwent PEG-6000 induced drought screening at -6 bar and a total of 32 genotypes were selected as tolerant. After 14 days of treatment, the nine observations viz. germination %, root length (cm), shoot length (cm), number of secondary roots, fresh weight (g), dry weight (g), shoot/root ratio, root/shoot ratio and vigour index were recorded. Variance analysis, revealing significant genetic variation among genotypes for all studied traits, indicating genetic variability. Post hoc analysis confirmed notable variation among treatments. Principal component analysis revealed three components, with the first three accounting for 88.89% of total variability. With respect to the biplot, the ten genotypes viz., IRGC109, IRGC403, IRGC448, IRGC461, IRGC466, IRGC486, IRGC508, IRGC518, IRGC527 and IRGC535 are the seedling stage drought tolerant genotypes based on shoot length, number of secondary roots and vigour index. Population structure classified the accessions into two subpopulations, reflecting diversity. The allele frequency divergence is 0.095 which is a measure of fixation index revealing that the moderate divergence is not extremely pronounced. Genetic diversity, assessed through 26 SSR markers selected from drought tolerant QTLs and markers related to vigour index, exhibited 100% polymorphism with 115 alleles and an average PIC value of 0.61 per primer. Shannon index varied between 0.34 (RM212) and 1.96 (RM252), averaging 1.18. Six SSR markers viz., RM246, RM302, RM252, RM219, RM251, and RM486 were associated with the six key traits viz., shoot length, root length, number of secondary roots, dry weight, shoot/root ratio, and root/shoot ratio respectively offering valuable resources for selecting drought-tolerant accessions as it provides the first step in the selection of genotypes based on the key traits. |
| format | Article |
| id | doaj-art-2c0a98837de94621a79ed637d433e63d |
| institution | Kabale University |
| issn | 1212-1975 1805-9325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj-art-2c0a98837de94621a79ed637d433e63d2025-01-14T12:46:40ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252025-01-0161112210.17221/12/2024-CJGPBcjg-202501-0001Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.)Shanmugam Manju Devi0John Amalraj Joel1Muthurajan Raveendran2Ramamoorthy Pushpam3Sengalan Muthuramu4Raman Pushpa5N. Sritharan6Periyasamy Prasanna7Ramalingam Suresh8Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, IndiaDirectorate of Research, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Forage Crops, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, IndiaAgricultural Research Station, Paramakudi, IndiaTamil Nadu Rice Research Institute, Aaduthurai, IndiaDepartment of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, IndiaWith climate change, plants face numerous stresses, notably drought for rice cultivation. Improving rice drought tolerance is vital for sustainable production in water-scarce regions. Identification of drought tolerant genotypes at the seedling stage of the crop contributes to build a climate resilient genotype during the period of water scarcity and under challenging environmental conditions. Hence, polyethylene glycol-6000 (PEG-6000) induced drought conditions could be used for testing the drought tolerance in rice at an earlier stage of the crop. Optimization of PEG-6000 concentration for screening index at -6 bar was done using three drought-tolerant and two drought-susceptible check varieties based on probit analysis. Subsequently, 100 rice landraces underwent PEG-6000 induced drought screening at -6 bar and a total of 32 genotypes were selected as tolerant. After 14 days of treatment, the nine observations viz. germination %, root length (cm), shoot length (cm), number of secondary roots, fresh weight (g), dry weight (g), shoot/root ratio, root/shoot ratio and vigour index were recorded. Variance analysis, revealing significant genetic variation among genotypes for all studied traits, indicating genetic variability. Post hoc analysis confirmed notable variation among treatments. Principal component analysis revealed three components, with the first three accounting for 88.89% of total variability. With respect to the biplot, the ten genotypes viz., IRGC109, IRGC403, IRGC448, IRGC461, IRGC466, IRGC486, IRGC508, IRGC518, IRGC527 and IRGC535 are the seedling stage drought tolerant genotypes based on shoot length, number of secondary roots and vigour index. Population structure classified the accessions into two subpopulations, reflecting diversity. The allele frequency divergence is 0.095 which is a measure of fixation index revealing that the moderate divergence is not extremely pronounced. Genetic diversity, assessed through 26 SSR markers selected from drought tolerant QTLs and markers related to vigour index, exhibited 100% polymorphism with 115 alleles and an average PIC value of 0.61 per primer. Shannon index varied between 0.34 (RM212) and 1.96 (RM252), averaging 1.18. Six SSR markers viz., RM246, RM302, RM252, RM219, RM251, and RM486 were associated with the six key traits viz., shoot length, root length, number of secondary roots, dry weight, shoot/root ratio, and root/shoot ratio respectively offering valuable resources for selecting drought-tolerant accessions as it provides the first step in the selection of genotypes based on the key traits.https://cjgpb.agriculturejournals.cz/artkey/cjg-202501-0001_unravelling-population-structure-and-marker-trait-association-using-ssr-markers-among-the-identified-drought-to.phpmolecular diversitypolyethylene glycolpolymorphic information contentshannon indexseed vigour |
| spellingShingle | Shanmugam Manju Devi John Amalraj Joel Muthurajan Raveendran Ramamoorthy Pushpam Sengalan Muthuramu Raman Pushpa N. Sritharan Periyasamy Prasanna Ramalingam Suresh Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.) Czech Journal of Genetics and Plant Breeding molecular diversity polyethylene glycol polymorphic information content shannon index seed vigour |
| title | Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.) |
| title_full | Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.) |
| title_fullStr | Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.) |
| title_full_unstemmed | Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.) |
| title_short | Unravelling population structure and marker trait association using SSR markers among the identified drought tolerant rice landraces (Oryza sativa L.) |
| title_sort | unravelling population structure and marker trait association using ssr markers among the identified drought tolerant rice landraces oryza sativa l |
| topic | molecular diversity polyethylene glycol polymorphic information content shannon index seed vigour |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-202501-0001_unravelling-population-structure-and-marker-trait-association-using-ssr-markers-among-the-identified-drought-to.php |
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