Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma
Tomatidine is isolated from the fruits of tomato plants and found to have anti-inflammatory effects in macrophages. In the present study, we investigated whether tomatidine suppresses airway hyperresponsiveness (AHR) and eosinophil infiltration in asthmatic mice. BALB/c mice were sensitized with ova...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/5261803 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850228168750792704 |
|---|---|
| author | Chieh-Ying Kuo Wen-Chung Huang Chian-Jiun Liou Li-Chen Chen Jiann-Jong Shen Ming-Ling Kuo |
| author_facet | Chieh-Ying Kuo Wen-Chung Huang Chian-Jiun Liou Li-Chen Chen Jiann-Jong Shen Ming-Ling Kuo |
| author_sort | Chieh-Ying Kuo |
| collection | DOAJ |
| description | Tomatidine is isolated from the fruits of tomato plants and found to have anti-inflammatory effects in macrophages. In the present study, we investigated whether tomatidine suppresses airway hyperresponsiveness (AHR) and eosinophil infiltration in asthmatic mice. BALB/c mice were sensitized with ovalbumin and treated with tomatidine by intraperitoneal injection. Airway resistance was measured by intubation analysis as an indication of airway responsiveness, and histological studies were performed to evaluate eosinophil infiltration in lung tissue. Tomatidine reduced AHR and decreased eosinophil infiltration in the lungs of asthmatic mice. Tomatidine suppressed Th2 cytokine production in bronchoalveolar lavage fluid. Tomatidine also blocked the expression of inflammatory and Th2 cytokine genes in lung tissue. In vitro, tomatidine inhibited proinflammatory cytokines and CCL11 production in inflammatory BEAS-2B bronchial epithelial cells. These results indicate that tomatidine contributes to the amelioration of AHR and eosinophil infiltration by blocking the inflammatory response and Th2 cell activity in asthmatic mice. |
| format | Article |
| id | doaj-art-2c0780c0dff84add85d44ea7c6597c3b |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2c0780c0dff84add85d44ea7c6597c3b2025-08-20T02:04:37ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/52618035261803Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of AsthmaChieh-Ying Kuo0Wen-Chung Huang1Chian-Jiun Liou2Li-Chen Chen3Jiann-Jong Shen4Ming-Ling Kuo5Department of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, TaiwanGraduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, TaiwanDivision of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, TaiwanSchool of Traditional Chinese Medicine, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, TaiwanDepartment of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, TaiwanTomatidine is isolated from the fruits of tomato plants and found to have anti-inflammatory effects in macrophages. In the present study, we investigated whether tomatidine suppresses airway hyperresponsiveness (AHR) and eosinophil infiltration in asthmatic mice. BALB/c mice were sensitized with ovalbumin and treated with tomatidine by intraperitoneal injection. Airway resistance was measured by intubation analysis as an indication of airway responsiveness, and histological studies were performed to evaluate eosinophil infiltration in lung tissue. Tomatidine reduced AHR and decreased eosinophil infiltration in the lungs of asthmatic mice. Tomatidine suppressed Th2 cytokine production in bronchoalveolar lavage fluid. Tomatidine also blocked the expression of inflammatory and Th2 cytokine genes in lung tissue. In vitro, tomatidine inhibited proinflammatory cytokines and CCL11 production in inflammatory BEAS-2B bronchial epithelial cells. These results indicate that tomatidine contributes to the amelioration of AHR and eosinophil infiltration by blocking the inflammatory response and Th2 cell activity in asthmatic mice.http://dx.doi.org/10.1155/2017/5261803 |
| spellingShingle | Chieh-Ying Kuo Wen-Chung Huang Chian-Jiun Liou Li-Chen Chen Jiann-Jong Shen Ming-Ling Kuo Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma Mediators of Inflammation |
| title | Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma |
| title_full | Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma |
| title_fullStr | Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma |
| title_full_unstemmed | Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma |
| title_short | Tomatidine Attenuates Airway Hyperresponsiveness and Inflammation by Suppressing Th2 Cytokines in a Mouse Model of Asthma |
| title_sort | tomatidine attenuates airway hyperresponsiveness and inflammation by suppressing th2 cytokines in a mouse model of asthma |
| url | http://dx.doi.org/10.1155/2017/5261803 |
| work_keys_str_mv | AT chiehyingkuo tomatidineattenuatesairwayhyperresponsivenessandinflammationbysuppressingth2cytokinesinamousemodelofasthma AT wenchunghuang tomatidineattenuatesairwayhyperresponsivenessandinflammationbysuppressingth2cytokinesinamousemodelofasthma AT chianjiunliou tomatidineattenuatesairwayhyperresponsivenessandinflammationbysuppressingth2cytokinesinamousemodelofasthma AT lichenchen tomatidineattenuatesairwayhyperresponsivenessandinflammationbysuppressingth2cytokinesinamousemodelofasthma AT jiannjongshen tomatidineattenuatesairwayhyperresponsivenessandinflammationbysuppressingth2cytokinesinamousemodelofasthma AT minglingkuo tomatidineattenuatesairwayhyperresponsivenessandinflammationbysuppressingth2cytokinesinamousemodelofasthma |