PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc
Objective: The roles of transcriptional factor PR/SET domain 1 (PRDM1) have been revealed in numerous tumor types. However, its effects on gastric cancer progression are still confusing. This study aimed to explore the roles and underlying mechanisms of PRDM1 in gastric cancer progression. Methods:...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523325001743 |
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| author | Hai Qin Manqin Yuan Yaqin Yuan Xue Liu Siye Yi |
| author_facet | Hai Qin Manqin Yuan Yaqin Yuan Xue Liu Siye Yi |
| author_sort | Hai Qin |
| collection | DOAJ |
| description | Objective: The roles of transcriptional factor PR/SET domain 1 (PRDM1) have been revealed in numerous tumor types. However, its effects on gastric cancer progression are still confusing. This study aimed to explore the roles and underlying mechanisms of PRDM1 in gastric cancer progression. Methods: In silico analysis was used to analyze gene expression levels, expression correlation, and the relationship with patients’ survivals. Functional experiments, including Western blot, RT-qPCR, sphere-formation ability, cytometry analysis, and tumorigenic analysis, were conducted to evaluate PRDM1’s effects on gastric cancer stemness. ChIP, luciferase reporter, and bioinformatic analysis were performed to reveal the underlying mechanisms. Results: we demonstrated that PRDM1 was highly expressed in gastric cancer tissues and cells. Knockdown of PRDM1 significantly attenuated gastric cancer stemness. Mechanistical studies indicated that PRDM1 directly bound to the promoter region of Myc, a critical contributor of cancer stem cells (CSCs), and thus enhanced Myc transcription activity. Conclusions: Taken together, this study indicates that PRDM1 could enhance gastric cancer stemness by increasing the transcriptional activity. |
| format | Article |
| id | doaj-art-2c015cede2e94775aa826b00c4be5d68 |
| institution | OA Journals |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-2c015cede2e94775aa826b00c4be5d682025-08-20T02:10:01ZengElsevierTranslational Oncology1936-52332025-09-015910244310.1016/j.tranon.2025.102443PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of MycHai Qin0Manqin Yuan1Yaqin Yuan2Xue Liu3Siye Yi4Department of Clinical Laboratory, Beijing Jishuitan Hospital Guizhou Hospital, No. 206, Sixian Street, Baiyun District, Guiyang City, Guizhou Province 550014, ChinaDepartment of Clinical Laboratory Medicine, Guizhou Medical University, Guiyang. Guizhou, ChinaMicrobiological Laboratory, Guizhou Center for Medical Device Testing, Guiyang, Guizhou, ChinaComprehensive Treatment Ward, Mudan People's Hospital of Heze, 2111 Kangzhuang Road, Mudan District, Heze, Shandong 274000, ChinaDepartment of Clinical Laboratory, Beijing Jishuitan Hospital Guizhou Hospital, No. 206, Sixian Street, Baiyun District, Guiyang City, Guizhou Province 550014, China; Corresponding author.Objective: The roles of transcriptional factor PR/SET domain 1 (PRDM1) have been revealed in numerous tumor types. However, its effects on gastric cancer progression are still confusing. This study aimed to explore the roles and underlying mechanisms of PRDM1 in gastric cancer progression. Methods: In silico analysis was used to analyze gene expression levels, expression correlation, and the relationship with patients’ survivals. Functional experiments, including Western blot, RT-qPCR, sphere-formation ability, cytometry analysis, and tumorigenic analysis, were conducted to evaluate PRDM1’s effects on gastric cancer stemness. ChIP, luciferase reporter, and bioinformatic analysis were performed to reveal the underlying mechanisms. Results: we demonstrated that PRDM1 was highly expressed in gastric cancer tissues and cells. Knockdown of PRDM1 significantly attenuated gastric cancer stemness. Mechanistical studies indicated that PRDM1 directly bound to the promoter region of Myc, a critical contributor of cancer stem cells (CSCs), and thus enhanced Myc transcription activity. Conclusions: Taken together, this study indicates that PRDM1 could enhance gastric cancer stemness by increasing the transcriptional activity.http://www.sciencedirect.com/science/article/pii/S1936523325001743PRDM1Gastric cancerStemnessMycTranscriptional factor |
| spellingShingle | Hai Qin Manqin Yuan Yaqin Yuan Xue Liu Siye Yi PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc Translational Oncology PRDM1 Gastric cancer Stemness Myc Transcriptional factor |
| title | PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc |
| title_full | PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc |
| title_fullStr | PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc |
| title_full_unstemmed | PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc |
| title_short | PRDM1 promotes the stemness of gastric cancer cells by enhancing the transactivation of Myc |
| title_sort | prdm1 promotes the stemness of gastric cancer cells by enhancing the transactivation of myc |
| topic | PRDM1 Gastric cancer Stemness Myc Transcriptional factor |
| url | http://www.sciencedirect.com/science/article/pii/S1936523325001743 |
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