Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis
Adiponectin is an adipose-secreted hormone that regulates energy homeostasis and is also involved in the control of the reproductive system. The goal of the present study was to investigate changes in adiponectin gene and protein expression in porcine ovarian structures during the oestrous cycle and...
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| Format: | Article |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/957076 |
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| author | Anna Maleszka Nina Smolinska Anna Nitkiewicz Marta Kiezun Katarzyna Chojnowska Kamil Dobrzyn Hubert Szwaczek Tadeusz Kaminski |
| author_facet | Anna Maleszka Nina Smolinska Anna Nitkiewicz Marta Kiezun Katarzyna Chojnowska Kamil Dobrzyn Hubert Szwaczek Tadeusz Kaminski |
| author_sort | Anna Maleszka |
| collection | DOAJ |
| description | Adiponectin is an adipose-secreted hormone that regulates energy homeostasis and is also involved in the control of the reproductive system. The goal of the present study was to investigate changes in adiponectin gene and protein expression in porcine ovarian structures during the oestrous cycle and to examine the effects of in vitro administration of adiponectin on basal and gonadotrophin- and/or insulin-induced secretion of ovarian steroid hormones. Both gene and protein expression of adiponectin were enhanced during the luteal phase of the cycle. Adiponectin affected basal secretion of progesterone by luteal cells, oestradiol by granulosa cells, and testosterone by theca interna cells. The gonadotrophin/insulin-induced release of progesterone from granulosa and theca interna cells and the release of oestradiol and androstenedione from theca cells was also modified by adiponectin. In conclusion, the presence of adiponectin mRNA and protein in the porcine ovary coupled with our previous results indicating adiponectin receptors expression suggest that adiponectin may locally affect ovarian functions. The changes in adiponectin expression throughout the oestrous cycle seem to be dependent on the hormonal status of pigs related to the stage of the oestrous cycle. The effect of adiponectin on ovarian steroidogenesis suggests that this adipokine influences reproductive functions in pigs. |
| format | Article |
| id | doaj-art-2be85b50cbaf47fb9aec941efac5bfa6 |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-2be85b50cbaf47fb9aec941efac5bfa62025-08-20T02:04:49ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/957076957076Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian SteroidogenesisAnna Maleszka0Nina Smolinska1Anna Nitkiewicz2Marta Kiezun3Katarzyna Chojnowska4Kamil Dobrzyn5Hubert Szwaczek6Tadeusz Kaminski7Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandDepartment of Animal Physiology, University of Warmia and Mazury in Olsztyn, Ulica Oczapowskiego 1A, Kortowo, 10-719 Olsztyn, PolandAdiponectin is an adipose-secreted hormone that regulates energy homeostasis and is also involved in the control of the reproductive system. The goal of the present study was to investigate changes in adiponectin gene and protein expression in porcine ovarian structures during the oestrous cycle and to examine the effects of in vitro administration of adiponectin on basal and gonadotrophin- and/or insulin-induced secretion of ovarian steroid hormones. Both gene and protein expression of adiponectin were enhanced during the luteal phase of the cycle. Adiponectin affected basal secretion of progesterone by luteal cells, oestradiol by granulosa cells, and testosterone by theca interna cells. The gonadotrophin/insulin-induced release of progesterone from granulosa and theca interna cells and the release of oestradiol and androstenedione from theca cells was also modified by adiponectin. In conclusion, the presence of adiponectin mRNA and protein in the porcine ovary coupled with our previous results indicating adiponectin receptors expression suggest that adiponectin may locally affect ovarian functions. The changes in adiponectin expression throughout the oestrous cycle seem to be dependent on the hormonal status of pigs related to the stage of the oestrous cycle. The effect of adiponectin on ovarian steroidogenesis suggests that this adipokine influences reproductive functions in pigs.http://dx.doi.org/10.1155/2014/957076 |
| spellingShingle | Anna Maleszka Nina Smolinska Anna Nitkiewicz Marta Kiezun Katarzyna Chojnowska Kamil Dobrzyn Hubert Szwaczek Tadeusz Kaminski Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis International Journal of Endocrinology |
| title | Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis |
| title_full | Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis |
| title_fullStr | Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis |
| title_full_unstemmed | Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis |
| title_short | Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis |
| title_sort | adiponectin expression in the porcine ovary during the oestrous cycle and its effect on ovarian steroidogenesis |
| url | http://dx.doi.org/10.1155/2014/957076 |
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