Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis
TIFY is one of the plant-specific transcription factors, which is extensively involved in regulating plant development, stress response, and biosynthesis of secondary metabolites. In this study, we identified 19 <i>DlTIFY</i> genes from the longan genome data. All of them contained the c...
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2025-04-01
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| author | Haowei Qing Ziang Wu Xiao Mo Jinjv Wei Yuyu Shi Huiqin Guo Jiongzhi Xu Feng Ding Shuwei Zhang |
| author_facet | Haowei Qing Ziang Wu Xiao Mo Jinjv Wei Yuyu Shi Huiqin Guo Jiongzhi Xu Feng Ding Shuwei Zhang |
| author_sort | Haowei Qing |
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| description | TIFY is one of the plant-specific transcription factors, which is extensively involved in regulating plant development, stress response, and biosynthesis of secondary metabolites. In this study, we identified 19 <i>DlTIFY</i> genes from the longan genome data. All of them contained the conserved TIFY domain, and these 19 <i>DlTIFYs</i> were distributed on 9 out of the 15 chromosomes of longan. A phylogenetic tree was constructed based on the TIFY protein sequences from <i>Arabidopsis</i>, rice, orange, and grape. They were clustered into six groups, with the TIFYs from longan showing the closest homology to those from orange. Duplication events were present between <i>DlTIFY1</i> and <i>DlTIFY5</i>, <i>DlTIFY4</i> and <i>DlTIFY6</i>, and <i>DlTIFY16</i> and <i>DlTIFY17</i>. There are several light-responsive elements, Abscisic Acid (ABA)-, Gibberellic Aci (GA)-, and Methyl Jasmonate (MeJA)-responsive elements, in the promoter regions of longan <i>TIFY</i> genes. Additionally, the flavonoid biosynthetic gene regulation elements were presented in the promoter of <i>DlTIFY7</i>, <i>DlTIFY10</i>, and <i>DlTIFY11</i>, suggesting their involvement in flavonoid synthesis regulation of longan. We also found that the expression of <i>DlTIFY7</i> in the stem and pericarp was significantly higher than that in other tissues. Interestingly, the outer edge of the corolla exhibited a green hue with a faint reddish tint across the corolla in transgenic tobacco plants overexpressing <i>DlTIFY7</i>. Subcellular localization experiments confirmed that DlTIFY7 is expressed in the nucleus. These findings suggest that <i>DlTIFY7</i> may serve as a novel candidate transcription factor negatively regulating anthocyanin synthesis in longan. This study provides valuable insights into the functional characterization of longan <i>DlTIFY</i> genes and lays a foundation for future research on their roles in regulating plant development and secondary metabolism. |
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| spelling | doaj-art-2be7a082c16f4d24acddc648b3d6b35f2025-08-20T02:17:24ZengMDPI AGBiology2079-77372025-04-0114436410.3390/biology14040364Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin SynthesisHaowei Qing0Ziang Wu1Xiao Mo2Jinjv Wei3Yuyu Shi4Huiqin Guo5Jiongzhi Xu6Feng Ding7Shuwei Zhang8Guangxi Key Laboratory of Genetic Improvement of Crops/Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaCollege of Agriculture, Guangxi University, Nanning 530007, ChinaGuangxi Key Laboratory of Genetic Improvement of Crops/Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Genetic Improvement of Crops/Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Genetic Improvement of Crops/Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Genetic Improvement of Crops/Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaCollege of Agriculture, Guangxi University, Nanning 530007, ChinaGuangxi Key Laboratory of Genetic Improvement of Crops/Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaInstitute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaTIFY is one of the plant-specific transcription factors, which is extensively involved in regulating plant development, stress response, and biosynthesis of secondary metabolites. In this study, we identified 19 <i>DlTIFY</i> genes from the longan genome data. All of them contained the conserved TIFY domain, and these 19 <i>DlTIFYs</i> were distributed on 9 out of the 15 chromosomes of longan. A phylogenetic tree was constructed based on the TIFY protein sequences from <i>Arabidopsis</i>, rice, orange, and grape. They were clustered into six groups, with the TIFYs from longan showing the closest homology to those from orange. Duplication events were present between <i>DlTIFY1</i> and <i>DlTIFY5</i>, <i>DlTIFY4</i> and <i>DlTIFY6</i>, and <i>DlTIFY16</i> and <i>DlTIFY17</i>. There are several light-responsive elements, Abscisic Acid (ABA)-, Gibberellic Aci (GA)-, and Methyl Jasmonate (MeJA)-responsive elements, in the promoter regions of longan <i>TIFY</i> genes. Additionally, the flavonoid biosynthetic gene regulation elements were presented in the promoter of <i>DlTIFY7</i>, <i>DlTIFY10</i>, and <i>DlTIFY11</i>, suggesting their involvement in flavonoid synthesis regulation of longan. We also found that the expression of <i>DlTIFY7</i> in the stem and pericarp was significantly higher than that in other tissues. Interestingly, the outer edge of the corolla exhibited a green hue with a faint reddish tint across the corolla in transgenic tobacco plants overexpressing <i>DlTIFY7</i>. Subcellular localization experiments confirmed that DlTIFY7 is expressed in the nucleus. These findings suggest that <i>DlTIFY7</i> may serve as a novel candidate transcription factor negatively regulating anthocyanin synthesis in longan. This study provides valuable insights into the functional characterization of longan <i>DlTIFY</i> genes and lays a foundation for future research on their roles in regulating plant development and secondary metabolism.https://www.mdpi.com/2079-7737/14/4/364<i>Dimocarpus longan</i>TIFY proteinssequence analysisanthocyanin synthesis |
| spellingShingle | Haowei Qing Ziang Wu Xiao Mo Jinjv Wei Yuyu Shi Huiqin Guo Jiongzhi Xu Feng Ding Shuwei Zhang Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis Biology <i>Dimocarpus longan</i> TIFY proteins sequence analysis anthocyanin synthesis |
| title | Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis |
| title_full | Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis |
| title_fullStr | Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis |
| title_full_unstemmed | Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis |
| title_short | Genome-Wide Identification of the TIFY Family in Longan and Their Potential Functional Analysis in Anthocyanin Synthesis |
| title_sort | genome wide identification of the tify family in longan and their potential functional analysis in anthocyanin synthesis |
| topic | <i>Dimocarpus longan</i> TIFY proteins sequence analysis anthocyanin synthesis |
| url | https://www.mdpi.com/2079-7737/14/4/364 |
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