Construction of a low molecular weight plasmid vector for the capture of genes encoding exported proteins
A pair of primers were designed to amplify a fragment of AMP gene, encoding merely mature part of β-lactamase with no promoter and signal peptide (△P△SP Amp), from pUC18. The gene was cloned into pGEM-T-EASY vector, and cut with EcoR Ⅰ. The recovered △P△SP Amp gene was then cloned and inserted betwe...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0127 |
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