Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1

Abstract Fusarium neocosmosporiellum causes alfalfa root rot, leading to significant economic losses. To explore the genetics of F. neocosmosporiellum, strain CA18-1 was isolated from infected alfalfa roots and subjected to whole-genome analysis. A scaffold-level reference genome of strain CA18-1 wa...

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Main Authors: Le Wang, Ruifang Jia, Jianjun Hao, Zhengqiang Chen, Xiaoluo Zhang, Na Wang, Shengze Wang, Yuanyuan Zhang, Kejian Lin
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05073-x
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author Le Wang
Ruifang Jia
Jianjun Hao
Zhengqiang Chen
Xiaoluo Zhang
Na Wang
Shengze Wang
Yuanyuan Zhang
Kejian Lin
author_facet Le Wang
Ruifang Jia
Jianjun Hao
Zhengqiang Chen
Xiaoluo Zhang
Na Wang
Shengze Wang
Yuanyuan Zhang
Kejian Lin
author_sort Le Wang
collection DOAJ
description Abstract Fusarium neocosmosporiellum causes alfalfa root rot, leading to significant economic losses. To explore the genetics of F. neocosmosporiellum, strain CA18-1 was isolated from infected alfalfa roots and subjected to whole-genome analysis. A scaffold-level reference genome of strain CA18-1 was assembled using both Nanopore long-read and Illumina short-read sequencing technologies. The assembled genome measured 63,424,297 base pairs (bp) in size, with a contig N50 of 6,480,858 bp and a contig N90 of 3,230,245 bp. A total of 10,486,459 bp repeat sequences and 28,006 protein-coding genes were predicted, of which 15,389 were functionally annotated. This study enhances our understanding of the genetic basis for F. neocosmosporiellum’s pathogenicity, providing a foundation for future studies on disease control and fungal evolution.
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spelling doaj-art-2bcd38993fa94510aa140b8a508299052025-08-20T01:49:42ZengNature PortfolioScientific Data2052-44632025-05-011211810.1038/s41597-025-05073-xScaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1Le Wang0Ruifang Jia1Jianjun Hao2Zhengqiang Chen3Xiaoluo Zhang4Na Wang5Shengze Wang6Yuanyuan Zhang7Kejian Lin8Key Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesKey Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesSchool of Food and Agriculture, University of MaineKey Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesInner Mongolia agricultural and animal husbandry machinery technology promotion stationKey Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesKey Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesKey Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesKey Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural SciencesAbstract Fusarium neocosmosporiellum causes alfalfa root rot, leading to significant economic losses. To explore the genetics of F. neocosmosporiellum, strain CA18-1 was isolated from infected alfalfa roots and subjected to whole-genome analysis. A scaffold-level reference genome of strain CA18-1 was assembled using both Nanopore long-read and Illumina short-read sequencing technologies. The assembled genome measured 63,424,297 base pairs (bp) in size, with a contig N50 of 6,480,858 bp and a contig N90 of 3,230,245 bp. A total of 10,486,459 bp repeat sequences and 28,006 protein-coding genes were predicted, of which 15,389 were functionally annotated. This study enhances our understanding of the genetic basis for F. neocosmosporiellum’s pathogenicity, providing a foundation for future studies on disease control and fungal evolution.https://doi.org/10.1038/s41597-025-05073-x
spellingShingle Le Wang
Ruifang Jia
Jianjun Hao
Zhengqiang Chen
Xiaoluo Zhang
Na Wang
Shengze Wang
Yuanyuan Zhang
Kejian Lin
Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
Scientific Data
title Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
title_full Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
title_fullStr Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
title_full_unstemmed Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
title_short Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1
title_sort scaffold level genome assembly of fusarium neocosmosporiellum strain ca18 1
url https://doi.org/10.1038/s41597-025-05073-x
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