In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm
Infections are a common post-operative ailment for patients who have received a dental implant or device and can be attributed to biofilm formation in tissue or on the implant. Many current solutions for oral hygiene have side effects and affect the natural oral microbiome. 2-heptylcyclopropane-1-ca...
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2024-11-01
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| author | Emily C. Montgomery Madelyn C. Wicker Tibirni Yusuf Elizabeth Matlock-Buchanan Tomoko Fujiwara Joel D. Bumgardner J. Amber Jennings |
| author_facet | Emily C. Montgomery Madelyn C. Wicker Tibirni Yusuf Elizabeth Matlock-Buchanan Tomoko Fujiwara Joel D. Bumgardner J. Amber Jennings |
| author_sort | Emily C. Montgomery |
| collection | DOAJ |
| description | Infections are a common post-operative ailment for patients who have received a dental implant or device and can be attributed to biofilm formation in tissue or on the implant. Many current solutions for oral hygiene have side effects and affect the natural oral microbiome. 2-heptylcyclopropane-1-carboxylic acid (2CP) is a medium-chain fatty acid and synthetic diffusible signaling factor that can prevent and disperse biofilm. The purpose of this work was to evaluate an immersion strategy for coating hydroxyapatite (HAp) with 2CP to prevent biofilm attachment on and around natural teeth and dental implants. The release profile of 2CP-loaded and 2CP+oral rinse-loaded HAp coupons (<i>n</i> = 6) was assessed by a 3-day exposure to phosphate buffered saline (PBS). Antimicrobial properties against <i>Porphyromonas gingivalis</i> and cytocompatibility of 2CP-loaded HAp coupons (<i>n</i> = 4) were also assessed alone and in combination with 0.12% chlorhexidine gluconate oral rinse. The majority of 2CP is released by 12 h. 2CP, oral rinse, and 2CP+oral rinse significantly reduced <i>P. gingivalis</i> viability, though direct contact assay demonstrates a significant reduction in Saos-2 viability for oral rinse and 2CP+oral rinse coupons. Immersion or rinsing hydroxyapatite with 2CP could inhibit biofilm-associated dental infections and prevent further complications including caries, gingivitis, and peri-implantitis. |
| format | Article |
| id | doaj-art-2bccee0f8b824570bad541da78af862c |
| institution | DOAJ |
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| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Hygiene |
| spelling | doaj-art-2bccee0f8b824570bad541da78af862c2025-08-20T02:53:33ZengMDPI AGHygiene2673-947X2024-11-014450051210.3390/hygiene4040037In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> BiofilmEmily C. Montgomery0Madelyn C. Wicker1Tibirni Yusuf2Elizabeth Matlock-Buchanan3Tomoko Fujiwara4Joel D. Bumgardner5J. Amber Jennings6Department of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USADepartment of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USADepartment of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USADepartment of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USADepartment of Chemistry, The University of Memphis, Memphis, TN 38152, USADepartment of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USADepartment of Biomedical Engineering, The University of Memphis, Memphis, TN 38152, USAInfections are a common post-operative ailment for patients who have received a dental implant or device and can be attributed to biofilm formation in tissue or on the implant. Many current solutions for oral hygiene have side effects and affect the natural oral microbiome. 2-heptylcyclopropane-1-carboxylic acid (2CP) is a medium-chain fatty acid and synthetic diffusible signaling factor that can prevent and disperse biofilm. The purpose of this work was to evaluate an immersion strategy for coating hydroxyapatite (HAp) with 2CP to prevent biofilm attachment on and around natural teeth and dental implants. The release profile of 2CP-loaded and 2CP+oral rinse-loaded HAp coupons (<i>n</i> = 6) was assessed by a 3-day exposure to phosphate buffered saline (PBS). Antimicrobial properties against <i>Porphyromonas gingivalis</i> and cytocompatibility of 2CP-loaded HAp coupons (<i>n</i> = 4) were also assessed alone and in combination with 0.12% chlorhexidine gluconate oral rinse. The majority of 2CP is released by 12 h. 2CP, oral rinse, and 2CP+oral rinse significantly reduced <i>P. gingivalis</i> viability, though direct contact assay demonstrates a significant reduction in Saos-2 viability for oral rinse and 2CP+oral rinse coupons. Immersion or rinsing hydroxyapatite with 2CP could inhibit biofilm-associated dental infections and prevent further complications including caries, gingivitis, and peri-implantitis.https://www.mdpi.com/2673-947X/4/4/37hydroxyapatitefatty aciddiffusible signaling factororal microbiome<i>Porphyromonas gingivalis</i>biofilm |
| spellingShingle | Emily C. Montgomery Madelyn C. Wicker Tibirni Yusuf Elizabeth Matlock-Buchanan Tomoko Fujiwara Joel D. Bumgardner J. Amber Jennings In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm Hygiene hydroxyapatite fatty acid diffusible signaling factor oral microbiome <i>Porphyromonas gingivalis</i> biofilm |
| title | In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm |
| title_full | In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm |
| title_fullStr | In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm |
| title_full_unstemmed | In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm |
| title_short | In Vitro Coating Hydroxyapatite with 2-Heptylcyclopropane-1-Carboxylic Acid Prevents <i>P. gingivalis</i> Biofilm |
| title_sort | in vitro coating hydroxyapatite with 2 heptylcyclopropane 1 carboxylic acid prevents i p gingivalis i biofilm |
| topic | hydroxyapatite fatty acid diffusible signaling factor oral microbiome <i>Porphyromonas gingivalis</i> biofilm |
| url | https://www.mdpi.com/2673-947X/4/4/37 |
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