A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions
Drought is a serious environmental challenge that reduces the productivity of valuable crops, including wheat. Brassinosteroids (BRs) is a group of phytohormones that have been used to enhance wheat drought tolerance. Wheat cultivars with different adaptation strategies could have their own specific...
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2025-03-01
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| author | Azamat Avalbaev Ruslan Yuldashev Anton Plotnikov Chulpan Allagulova |
| author_facet | Azamat Avalbaev Ruslan Yuldashev Anton Plotnikov Chulpan Allagulova |
| author_sort | Azamat Avalbaev |
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| description | Drought is a serious environmental challenge that reduces the productivity of valuable crops, including wheat. Brassinosteroids (BRs) is a group of phytohormones that have been used to enhance wheat drought tolerance. Wheat cultivars with different adaptation strategies could have their own specific drought tolerance mechanisms, and could react differently to treatment with growth regulators. In this work, the effect of seed pretreatment with 0.4 µM 24-epibrassinolide (EBR) was investigated in two wheat (<i>Triticum aestivum</i> L.) cultivars contrasting in drought behavior, tolerant Ekada 70 (cv. E70) and sensitive Zauralskaya Zhemchuzhina (cv. ZZh), in early ontogenesis under dehydration (PEG-6000) or soil drought conditions. EBR pretreatment mitigated the stress-induced inhibition of seedling emergence and growth, as well as membrane damage in cv.E70 but not in ZZh. An enzyme-linked immunosorbent assay (ELISA) revealed substantial changes in hormonal balance associated with ABA accumulation and a drop in the levels of IAA and cytokinins (CKs) in drought-subjected seedlings of both cultivars, especially ZZh. EBR-pretreatment reduced drought-induced hormone imbalance in cv. E70, while it did not have the same effect on ZZh. EBR-induced changes in the content of wheat germ agglutinin (WGA) belonging to the protective proteins in E70 seedlings suggest its contribution to EBR-dependent adaptive responses. The absence of a detectable protective effect of EBR on the ZZh cultivar may be associated with its insensitivity to pre-sowing EBR treatment. |
| format | Article |
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| issn | 2223-7747 |
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| publishDate | 2025-03-01 |
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| spelling | doaj-art-2bc59dc2371447a4990ceefdf67850822025-08-20T02:42:28ZengMDPI AGPlants2223-77472025-03-0114686910.3390/plants14060869A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit ConditionsAzamat Avalbaev0Ruslan Yuldashev1Anton Plotnikov2Chulpan Allagulova3Institute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, Lit.1e, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, Lit.1e, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, Lit.1e, Ufa 450054, RussiaInstitute of Biochemistry and Genetics—Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Prospect Oktyabrya 71, Lit.1e, Ufa 450054, RussiaDrought is a serious environmental challenge that reduces the productivity of valuable crops, including wheat. Brassinosteroids (BRs) is a group of phytohormones that have been used to enhance wheat drought tolerance. Wheat cultivars with different adaptation strategies could have their own specific drought tolerance mechanisms, and could react differently to treatment with growth regulators. In this work, the effect of seed pretreatment with 0.4 µM 24-epibrassinolide (EBR) was investigated in two wheat (<i>Triticum aestivum</i> L.) cultivars contrasting in drought behavior, tolerant Ekada 70 (cv. E70) and sensitive Zauralskaya Zhemchuzhina (cv. ZZh), in early ontogenesis under dehydration (PEG-6000) or soil drought conditions. EBR pretreatment mitigated the stress-induced inhibition of seedling emergence and growth, as well as membrane damage in cv.E70 but not in ZZh. An enzyme-linked immunosorbent assay (ELISA) revealed substantial changes in hormonal balance associated with ABA accumulation and a drop in the levels of IAA and cytokinins (CKs) in drought-subjected seedlings of both cultivars, especially ZZh. EBR-pretreatment reduced drought-induced hormone imbalance in cv. E70, while it did not have the same effect on ZZh. EBR-induced changes in the content of wheat germ agglutinin (WGA) belonging to the protective proteins in E70 seedlings suggest its contribution to EBR-dependent adaptive responses. The absence of a detectable protective effect of EBR on the ZZh cultivar may be associated with its insensitivity to pre-sowing EBR treatment.https://www.mdpi.com/2223-7747/14/6/869wheat (<i>Triticum aestivum</i> L.) cultivarsdrought adaptation24-epibrassinolidephytohormoneswheat germ agglutinin |
| spellingShingle | Azamat Avalbaev Ruslan Yuldashev Anton Plotnikov Chulpan Allagulova A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions Plants wheat (<i>Triticum aestivum</i> L.) cultivars drought adaptation 24-epibrassinolide phytohormones wheat germ agglutinin |
| title | A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions |
| title_full | A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions |
| title_fullStr | A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions |
| title_full_unstemmed | A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions |
| title_short | A Comparative Analysis of the Effect of 24-Epibrassinolide on the Tolerance of Wheat Cultivars with Different Drought Adaptation Strategies Under Water Deficit Conditions |
| title_sort | comparative analysis of the effect of 24 epibrassinolide on the tolerance of wheat cultivars with different drought adaptation strategies under water deficit conditions |
| topic | wheat (<i>Triticum aestivum</i> L.) cultivars drought adaptation 24-epibrassinolide phytohormones wheat germ agglutinin |
| url | https://www.mdpi.com/2223-7747/14/6/869 |
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