First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan
Background: Patients with haematological disorders, such as sickle cell anaemia, are at an elevated risk of transient aplastic crisis due to parvovirus B19 infection. The virus targets specific integration sites in the human genome, disrupting cellular division. However, the molecular mechanisms of...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Journal of Infection and Public Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034125000310 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864383253512192 |
|---|---|
| author | Khalid Salman Sittna Hayder El Nagar Abdel Rahim M. El Hussein Mohammed A. El Hussein Hadi M. Yassine Hebah A. Al Khatib Mashael Ali Al- Badr Ibrahim Farah Khalid A. Enan |
| author_facet | Khalid Salman Sittna Hayder El Nagar Abdel Rahim M. El Hussein Mohammed A. El Hussein Hadi M. Yassine Hebah A. Al Khatib Mashael Ali Al- Badr Ibrahim Farah Khalid A. Enan |
| author_sort | Khalid Salman |
| collection | DOAJ |
| description | Background: Patients with haematological disorders, such as sickle cell anaemia, are at an elevated risk of transient aplastic crisis due to parvovirus B19 infection. The virus targets specific integration sites in the human genome, disrupting cellular division. However, the molecular mechanisms of infection remain poorly understood. Aim: This study aimed to determine the prevalence of human parvovirus B19 among patients with sickle cell anaemia in Khartoum State, Sudan. Methods: Ninety patients (aged <5 to >15 years) with sickle cell disease attending Gaafer Ibnouaf Children’s Hospital between November 2016 and February 2017 were recruited. Sera and plasma samples were analyzed. IgG and IgM antibodies were measured using ELISA, and viral DNA was detected in plasma using nested-PCR. Phylogenetic analysis of sequenced B19 strains focused on the overlapping region of the minor (VP1) and major (VP2) capsid protein genes. Results: Anti-parvovirus B19 IgG antibodies were detected in 57 of 90 patients (63.3 %), while IgM antibodies were present in 7 (7.8 %). Viral DNA was identified in 23 (25.5 %) patients. Among the 23 DNA-positive patients, 7 (30 %) were seronegative for both IgG and IgM antibodies, highlighting the importance of molecular diagnostics in identifying active infections, especially in early stages. Children under 5 years of age exhibited a higher nucleic acid detection rate compared to older age groups, suggesting the importance of molecular testing in younger patients particularly in the early detection of Parvovirus B19 during the acute phase of infection, before the body has developed detectable antibodies and also in immunocompromised children, who may not mount an antibody response detectable by serological methods. Phylogenetic analysis revealed two distinct Sudanese clusters: one (50 % of sequences) formed a unique clade with low similarity to existing genotypes, while the other (50 %) closely resembled genotype 1 A sequences from Iraq, Iran, and Tanzania. Conclusion: Parvovirus B19 antibodies and DNA were detected at high prevalence among Sudanese children with sickle cell anaemia. Screening for parvovirus B19 is critical for patients requiring blood transfusions, particularly those with haematological disorders. This study provides the first report of parvovirus B19 detection, sequencing, and characterization among Sudanese patients with sickle cell anaemia. |
| format | Article |
| id | doaj-art-2bb2791399884cab93fac5e0f8f2503f |
| institution | Kabale University |
| issn | 1876-0341 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj-art-2bb2791399884cab93fac5e0f8f2503f2025-02-09T05:00:00ZengElsevierJournal of Infection and Public Health1876-03412025-04-01184102682First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, SudanKhalid Salman0Sittna Hayder El Nagar1Abdel Rahim M. El Hussein2Mohammed A. El Hussein3Hadi M. Yassine4Hebah A. Al Khatib5Mashael Ali Al- Badr6Ibrahim Farah7Khalid A. Enan8Department of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, SudanOmdurman Teaching Hospital, Ministry of Health, Khartoum, SudanDepartment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, SudanDepartment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, SudanBiomedical Research Center, Qatar University, P.O. Box 2713, Doha, QatarBiomedical Research Center, Qatar University, P.O. Box 2713, Doha, QatarPreventive Reference Laboratory, Department of Health Protection & Communicable Diseases Control, Ministry of Public Health, Doha, QatarPreventive Reference Laboratory, Department of Health Protection & Communicable Diseases Control, Ministry of Public Health, Doha, QatarDepartment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan; Preventive Reference Laboratory, Department of Health Protection & Communicable Diseases Control, Ministry of Public Health, Doha, Qatar; Corresponding author at: Preventive Reference Laboratory, Department of Health Protection & Communicable Diseases Control, Ministry of Public Health, Doha, Qatar.Background: Patients with haematological disorders, such as sickle cell anaemia, are at an elevated risk of transient aplastic crisis due to parvovirus B19 infection. The virus targets specific integration sites in the human genome, disrupting cellular division. However, the molecular mechanisms of infection remain poorly understood. Aim: This study aimed to determine the prevalence of human parvovirus B19 among patients with sickle cell anaemia in Khartoum State, Sudan. Methods: Ninety patients (aged <5 to >15 years) with sickle cell disease attending Gaafer Ibnouaf Children’s Hospital between November 2016 and February 2017 were recruited. Sera and plasma samples were analyzed. IgG and IgM antibodies were measured using ELISA, and viral DNA was detected in plasma using nested-PCR. Phylogenetic analysis of sequenced B19 strains focused on the overlapping region of the minor (VP1) and major (VP2) capsid protein genes. Results: Anti-parvovirus B19 IgG antibodies were detected in 57 of 90 patients (63.3 %), while IgM antibodies were present in 7 (7.8 %). Viral DNA was identified in 23 (25.5 %) patients. Among the 23 DNA-positive patients, 7 (30 %) were seronegative for both IgG and IgM antibodies, highlighting the importance of molecular diagnostics in identifying active infections, especially in early stages. Children under 5 years of age exhibited a higher nucleic acid detection rate compared to older age groups, suggesting the importance of molecular testing in younger patients particularly in the early detection of Parvovirus B19 during the acute phase of infection, before the body has developed detectable antibodies and also in immunocompromised children, who may not mount an antibody response detectable by serological methods. Phylogenetic analysis revealed two distinct Sudanese clusters: one (50 % of sequences) formed a unique clade with low similarity to existing genotypes, while the other (50 %) closely resembled genotype 1 A sequences from Iraq, Iran, and Tanzania. Conclusion: Parvovirus B19 antibodies and DNA were detected at high prevalence among Sudanese children with sickle cell anaemia. Screening for parvovirus B19 is critical for patients requiring blood transfusions, particularly those with haematological disorders. This study provides the first report of parvovirus B19 detection, sequencing, and characterization among Sudanese patients with sickle cell anaemia.http://www.sciencedirect.com/science/article/pii/S1876034125000310Sickle cell anaemiaParvovirus B19SeroprevalenceGenotypesNested PCR |
| spellingShingle | Khalid Salman Sittna Hayder El Nagar Abdel Rahim M. El Hussein Mohammed A. El Hussein Hadi M. Yassine Hebah A. Al Khatib Mashael Ali Al- Badr Ibrahim Farah Khalid A. Enan First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan Journal of Infection and Public Health Sickle cell anaemia Parvovirus B19 Seroprevalence Genotypes Nested PCR |
| title | First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan |
| title_full | First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan |
| title_fullStr | First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan |
| title_full_unstemmed | First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan |
| title_short | First report of serological, molecular detection, and characterization of human parvovirus B19 infections among sickle cell anaemia patients in Khartoum State, Sudan |
| title_sort | first report of serological molecular detection and characterization of human parvovirus b19 infections among sickle cell anaemia patients in khartoum state sudan |
| topic | Sickle cell anaemia Parvovirus B19 Seroprevalence Genotypes Nested PCR |
| url | http://www.sciencedirect.com/science/article/pii/S1876034125000310 |
| work_keys_str_mv | AT khalidsalman firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT sittnahayderelnagar firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT abdelrahimmelhussein firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT mohammedaelhussein firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT hadimyassine firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT hebahaalkhatib firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT mashaelalialbadr firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT ibrahimfarah firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan AT khalidaenan firstreportofserologicalmoleculardetectionandcharacterizationofhumanparvovirusb19infectionsamongsicklecellanaemiapatientsinkhartoumstatesudan |